Hepatic differentiation of human bone marrow‐derived UE7T‐13 cells: Effects of cytokines and CCN family gene expression

Shimomura, Tokio; Yoshida, Yoko; Sakabe, Tomohiko; Ishii, Kyoko; Gonda, Kazue; Murai, Rie; Takubo, Kazuko; Tsuchiya, Hiroyuki; Hoshikawa, Yasushi; Kurimasa, Akihiro; Hisatome, Ichiro; Uyama, Taro; Umezawa, Akihiro; Shiota, Goshi

doi:10.1111/j.1872-034x.2007.00162.x

Cited by 40 publications

(39 citation statements)

References 30 publications

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“…During the second stage, from 9th to 14th day , albumin (ALB), ornithine transcarbamylase (TAT) as well as mRNA levels for VII clotting factor, asialoglycoprotein receptor 1 (ASGPR1), and CYP1A2 member of cytochrome P450 superfamily production are increased while AFP levels are reduced [65–68]. The last stage of maturation process, from 15th to 21st day , presents hepatic-like cells with functional properties unique to hepatocytes such as increased detoxifying ability, lipid and glycogen storage capacity, urea production, and bile acid secretion [65–68]. …”

Section: Resultsmentioning

confidence: 99%

Mesenchymal Stem Cells Transplantation following Partial Hepatectomy: A New Concept to Promote Liver Regeneration—Systematic Review of the Literature Focused on Experimental Studies in Rodent Models

Papanikolaou

Katselis

Αποστόλου

et al. 2017

Stem Cells International

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Mesenchymal stem cells (MSCs) are an attractive source for regenerative medicine because they are easily accessible through minimally invasive methods and have the potential to enhance liver regeneration (LG) and improve liver function, following partial hepatectomy (PH) and acute or chronic liver injury. A systematic review of the literature was conducted for articles published up to September 1st, 2016, using the MEDLINE database. The keywords that were used in various combinations were as follows: “Mesenchymal stem cells”, “transplantation”, “stem cells”, “adipose tissue derived stem cells”, “bone marrow-derived stem cells”, “partial hepatectomy”, “acute liver failure”, “chronic liver failure”, “liver fibrosis”, “liver cirrhosis”, “rats”, “mice”, and “liver regeneration”. All introduced keywords were searched for separately in MeSH Database to control relevance and terminological accuracy and validity. A total of 41 articles were identified for potential inclusion and reviewed in detail. After a strict selection process, a total of 28 articles were excluded, leaving 13 articles to form the basis of this systematic review. MSCs transplantation promoted LG and improved liver function. Furthermore, MSCs had the ability to differentiate in hepatocyte-like cells, increase survival, and protect hepatocytes by paracrine mechanisms. MSCs transplantation may provide beneficial effects in the process of LG after PH and acute or chronic liver injury. They may represent a new therapeutic option to treat posthepatectomy acute liver failure.

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Section: Resultsmentioning

confidence: 99%

Mesenchymal Stem Cells Transplantation following Partial Hepatectomy: A New Concept to Promote Liver Regeneration—Systematic Review of the Literature Focused on Experimental Studies in Rodent Models

Papanikolaou

Katselis

Αποστόλου

et al. 2017

Stem Cells International

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“…SH9 human PDL cells were maintained in α-modified minimum essential medium (α-MEM; Gibco, Life Technologies, Gaithersburg, MD, USA) supplemented with 10% fetal bovine serum (FBS; Gibco), 50 µg/ml streptomycin, and 50 U/ml penicillin. UE7T-13 cells, which are human bone marrow-derived MSCs infected with retroviruses expressing papillomavirus E7 and hTERT to prolong their lifespan (13,14), were purchased from the Health Science Research Resources Bank (JCRB1154, Japan Health Sciences Foundation, Tokyo, Japan) and cultured in α-MEM. All cultures were maintained at 37˚C in a humidified atmosphere of 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

Differential effects of TGF-β1 and FGF-2 on SDF-1α expression in human periodontal ligament cells derived from deciduous teeth in vitro

et al. 2012

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“…cells was prolonged by infecting them with a retrovirus containing human papillomavirus E7 and telomerase reverse transcriptase (hTERT) cDNAs (Mori et al, 2005;Shimomura et al, 2007;Ishii et al, 2008;Takeuchi et al, 2007). We first tested the ability of hESF9 medium, which we had developed for use with hES cells, to support the growth of UE7T-13 cells.…”

mentioning

confidence: 99%

Growth factor-defined culture medium for human mesenchymal stem cells

Mimura¹,

Kimura²,

Hirata³

et al. 2011

Int. J. Dev. Biol.

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Human bone marrow-derived mesenchymal stem cells (hMSCs) are potential cellular sources of therapeutic stem cells as they have the ability to proliferate and differentiate into a wide array of mesenchymal cell types such as osteoblasts, chondroblasts and adipocytes. hMSCs have been used clinically to treat patients with graft vs. host disease, osteogenesis imperfect, or alveolar cleft, suggesting that transplantation of hMSCs is comparatively safe as a stem cell-based therapy. However, conventional culture medium for hMSCs contains fetal bovine serum (FBS). In the present study, we developed a growth factor-defined, serum-free medium for culturing hMSCs. Under these conditions, TGF-    1 promoted proliferation of hMSCs. The expanded hMSC population expressed the human pluripotency markers SSEA-3, -4, NANOG, OCT3/4 and SOX2. Furthermore, double positive cells for SSEA-3 and a mesenchymal cell marker, CD105, were detected in the population. The potential to differentiate into osteoblasts and adipocytes was confirmed. This work provides a useful tool to understand the basic biological properties of hMSCs in culture.

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Hepatic differentiation of human bone marrow‐derived UE7T‐13 cells: Effects of cytokines and CCN family gene expression

Abstract: The present study suggests that aFGF/bFGF/HGF/type IV collagen coating is the efficient condition for hepatic differentiation of UE7T-13 cells, and that WISP1 and WISP2 play an important role in hepatic transdifferentiation of these cells.

Cited by 40 publications

References 30 publications

Mesenchymal Stem Cells Transplantation following Partial Hepatectomy: A New Concept to Promote Liver Regeneration—Systematic Review of the Literature Focused on Experimental Studies in Rodent Models

Mesenchymal Stem Cells Transplantation following Partial Hepatectomy: A New Concept to Promote Liver Regeneration—Systematic Review of the Literature Focused on Experimental Studies in Rodent Models

Differential effects of TGF-β1 and FGF-2 on SDF-1α expression in human periodontal ligament cells derived from deciduous teeth in vitro

Growth factor-defined culture medium for human mesenchymal stem cells

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