Hepatic interferon γ and tumor necrosis factor a expression in infants with neonatal cholestasis and cytomegalovirus infection

Meshram, Himali; Velhal, Shilpa; Padwal, Varsha; Sutar, Jyoti; Kadam, Ravi N.; Pereira, J.K.; Bhonde, Gauri; Karandikar, Kalyani; Bhor, Vikrant M.; Patel, Vainav; Shetty, Naman S; Shah, Ira

doi:10.5114/ceh.2020.102172

Cited by 6 publications

(3 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Finally, a total of 19 studies published from 1980 to 2020, which met the eligibility for data extraction and analyses were used for the quantitative synthesis; nine studies from Asia, five studies from Europe, four studies from the Americas, and a study from Africa. 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 The main characteristics of these included studies are shown in Table 1 .…”

Section: Resultsmentioning

confidence: 99%

Detection of Cytomegalovirus Infection in Infants with Biliary Atresia: A Meta-analysis

et al. 2021

View full text Add to dashboard Cite

Objectives Biliary atresia (BA) is the most common indication of liver transplantation in children. Several reports attributed BA to both prenatal and perinatal etiologies, including a viral infection-induced autoimmune response that targets the bile ducts. Cytomegalovirus (CMV) remains the most common virus being linked to BA. This meta-analysis aimed to estimate to what extent CMV infection is detected in patients with BA. Methods This study was conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. The databases of MEDLINE, Embase, Scopus, WHO-Virtual Health Library (VHL), ScienceDirect, and Google Scholar were used for the systematic search. The risk of bias was assessed using the Newcastle–Ottawa scale. Random effects model was used to estimate the pooled prevalence estimate with the corresponding 95% confidence interval (CI) using Comprehensive Meta-Analysis Software version 3.3. Results A total of 19 studies that fulfilled the eligibility criteria were included in the meta-analysis. The total number of infants with BA was 630 patients, and the pooled overall prevalence of CMV infection among them was 25.4% (95% CI: 15.9%–38.0%). There was high heterogeneity among studies (I2 = 85.1%, p < .001), and subgroup analyses showed significant regional differences (X2 = 48.9, p <.001). Data on the prognosis of CMV-associated BA were scarce and obtainable from few studies that suggested an association between detection of CMV infection and poor prognosis of BA. Conclusions The limited available data demonstrates that the rate of detection of CMV infection is high in infants with BA. There is still a need for large studies with appropriate controls for obtaining more reliable results about the various aspects of the association between CMV infection and BA.

show abstract

Section: Resultsmentioning

confidence: 99%

Detection of Cytomegalovirus Infection in Infants with Biliary Atresia: A Meta-analysis

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Therefore, inflammatory cytokines play a vital role in the process of hepatic fibrosis of biliary atresia. In BA patients and BA model rats, IL-6, IL-1 β , and TNF- α significantly increased [ 21 , 22 , 23 ]. In the process of liver injury, IL-6 is released from bile duct epithelial cells, which directly promotes cell regeneration and proliferation [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

Combination of Pirfenidone and Andrographolide Ameliorates Hepatic Stellate Cell Activation and Liver Fibrosis by Mediating TGF‐β/Smad Signaling Pathway

Xu,

Ma,

Zhou

et al. 2024

Analytical Cellular Pathology

View full text Add to dashboard Cite

Background. Biliary atresia (BA) is a devastating congenital disease characterized by inflammation and progressive liver fibrosis. Activation of hepatic stellate cells (HSCs) plays a central role in the pathogenesis of hepatic fibrosis. Our study aimed to investigate the pharmacological effect and potential mechanism of pirfenidone (PFD) and andrographolide (AGP) separately and together on liver fibrosis of BA. Materials and Methods. The bile ducts of male C57BL/6J mice were ligated or had the sham operation. The in vivo effects of PFD and/or AGP on liver fibrosis of BA were evaluated. Human hepatic stellate cells (LX‐2) were also treated with PFD and/or AGP in vitro. Results. PFD and/or AGP ameliorates liver fibrosis and inflammation in the mice model of BA, as evidenced by significant downregulated in the accumulation of collagen fibers, hepatic fibrosis markers (α‐SMA, collagen I, and collagen IV), and inflammatory markers (IL‐1β, IL‐6, and TNF‐α). Moreover, compared with monotherapy, these changes are more obvious in the combined treatment of PFD and AGP. Consistent with animal experiments, hepatic fibrosis markers (α‐SMA, collagen I, and CTGF) and inflammatory markers (IL‐1β, IL‐6, and TNF‐α) were significantly decreased in activated LX‐2 cells after PFD and/or AGP treatment. In addition, PFD and/or AGP inhibited the activation of HSCs by blocking the TGF‐β/Smad signaling pathway, and the combined treatment of PFD and AGP synergistically inhibited the phosphorylation of Smad2 and Smad3. Conclusion. The combined application of PFD and AGP exerted superior inhibitive effects on HSC activation and liver fibrosis by mediating the TGF‐β/Smad signaling pathway as compared to monotherapy. Therefore, the combination of PFD and AGP may be a promising treatment strategy for liver fibrosis in BA.

show abstract

“…Cholestasis in term infants is a rare condition of different causes where biliary atresia is the most common (11). CMV has been associated with biliary atresia and other cholestatic conditions in infancy (12)(13)(14)(15)(16). In preterm infants, cholestasis is far more common than in term infants, and the condition is related to prolonged parenteral nutrition, often due to feeding intolerance secondary to bowel disease such as NEC with subsequent intestinal failure (17)(18)(19), but the potential importance of CMV in this condition is not well-investigated.…”

Section: Introductionmentioning

confidence: 99%

High Rate of Cytomegalovirus Detection in Cholestatic Preterm Infants

et al. 2021

View full text Add to dashboard Cite

Objectives: To evaluate the prevalence of cytomegalovirus (CMV) infection in preterm infants with cholestasis.Study design: Preterm infants (<37 weeks gestational age) with cholestasis were tested for CMV DNA using Taqman PCR in blood cells from sedimented whole blood, plasma, and urine. Infants were regarded as positive for CMV if any sample was tested positive. Their mothers were tested for CMV serostatus simultaneously. A control group of non-cholestatic preterm infants, and their mothers, were tested at a similar age.Results: A total of 69 preterm infants with a median gestational age of 26 weeks and 5 days were included, 45 cholestatic and 24 non-cholestatic. Of the cholestatic infants, 31/45 (69%) were CMV positive vs. 3/24 (13%) of the non-cholestatic infants (p < 0.001). Cholestatic infants were equally preterm as the non-cholestatic ones, but were more severely ill. After adjusting for the risk factors necrotizing enterocolitis, prolonged parenteral nutrition, and gestational age, being CMV positive remained significantly associated with cholestasis in a multivariable logistic regression model. Characteristics of CMV-positive and -negative cholestatic infants showed differences only for necrotizing enterocolitis, occurring in 55% (17/31) of CMV positive vs. 21% (3/14) of CMV negative (p = 0.054), and mortality. Eight cholestatic CMV-positive infants died (26%) vs. none of the CMV-negative infants (p = 0.044).Conclusions: CMV DNA was detected in two out of three cholestatic preterm infants, by far more often than in the non-cholestatic control group. Cholestasis with simultaneous detection of CMV DNA may be associated with increased mortality.

show abstract

Hepatic interferon γ and tumor necrosis factor a expression in infants with neonatal cholestasis and cytomegalovirus infection

Cited by 6 publications

References 22 publications

Detection of Cytomegalovirus Infection in Infants with Biliary Atresia: A Meta-analysis

Detection of Cytomegalovirus Infection in Infants with Biliary Atresia: A Meta-analysis

Combination of Pirfenidone and Andrographolide Ameliorates Hepatic Stellate Cell Activation and Liver Fibrosis by Mediating TGF‐β/Smad Signaling Pathway

High Rate of Cytomegalovirus Detection in Cholestatic Preterm Infants

Contact Info

Product

Resources

About