Hepatic stereology of schistosomiasis mansoni infected-mice fed a high-fat diet

Neves, Renata Heisler; Alencar, Alba Cristina Miranda de Barros; Águila, Márcia Barbosa; Mandarim-de-Lacerda, Carlos Alberto; Machado-Silva, José Roberto; Gomes, Delir Corrêa

doi:10.1590/s0074-02762006000900039

Cited by 27 publications

(30 citation statements)

References 44 publications

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“…Although proved relevant in clinical studies (6), very little is known about stereological data in hepatic schistosomiasis (7,8). The diet of the host has been suggested to be a stimulation factor to hepatic regeneration (15).…”

Section: Discussionmentioning

confidence: 99%

“…The diet of the host has been suggested to be a stimulation factor to hepatic regeneration (15). Schistosomiasis also represents a stimulus during the acute phase of the infection (8,15). The present findings showed that both malnutrition and schistosomiasis led to adverse effects on liver morphology.…”

Section: Discussionmentioning

confidence: 99%

“…Mice were exposed subcutaneously to ~50 S. mansoni cercariae (BH strain, Brazil) 15 days after ingesting their respective diets. The cercariae were obtained from laboratory-raised and -infected Biomphalaria glabrata, as described (8).…”

Section: Animalsmentioning

confidence: 99%

“…Details of the procedures are given elsewhere (8). Briefly, hepatic fragments (minimum of three fragments per animal) were embedded together and oriented to provide a random cut when sectioned.…”

Section: Stereological Studymentioning

confidence: 99%

“…In a previous investigation, stereology also proved to be useful for experimental evaluation of the nutritional effects of a high-fat diet in murine schistosomiasis (8). On the basis of the considerations, the objective of the present study was to test liver status following PZQ administration to S. mansoni-infected mice fed a low-protein diet, using stereology parameters.…”

mentioning

confidence: 99%

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Effect of praziquantel administration on hepatic stereology of mice infected with Schistosoma mansoni and fed a low-protein diet

Barros

Costa-Silva

Biolchini

et al. 2009

Braz J Med Biol Res

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A study was undertaken to investigate the effect of administering praziquantel (PZQ), focusing on the liver stereological findings of malnourished mice infected with Schistosoma mansoni. Thirty female Swiss Webster mice (age: 21 days; weight: 8-14 g) were fed either a low-protein diet (8%) or standard chow (22% protein) for 15 days. Five mice in each group were infected with 50 cercariae each of the BH strain (Brazil). PZQ therapy (80 mg/kg body weight, per day) was started on the 50th day of infection and consisted of daily administration for 5 days. Volume density (hepatocytes, sinusoids and hepatic fibrosis) was determined by stereology using a light microscope. Body weight gain and total serum albumin levels were always lower in undernourished mice. Our stereological study demonstrated that treatment increased both volume density of hepatocytes in mice fed standard chow (47.56%, treated group and 12.06%, control) and low-protein chow (30.98%, treated group and 21.44%, control), and hepatic sinusoids [standard chow (12.52%, treated group and 9.06%, control), low-protein chow (14.42%, treated group and 8.46%, control)], while hepatic fibrosis was reduced [standard chow (39.92%, treated group and 78.88%, control) and low-protein chow (54.60%, treated group and 70.10%, control)]. On the other hand, mice fed low-protein chow decreased density volume of hepatocytes and hepatic fibrosis. In conclusion, our findings indicate that treatment with PZQ ameliorates hepatic schistosomiasis pathology even in mice fed a low-protein diet.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Animalsmentioning

confidence: 99%

Section: Stereological Studymentioning

confidence: 99%

mentioning

confidence: 99%

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Effect of praziquantel administration on hepatic stereology of mice infected with Schistosoma mansoni and fed a low-protein diet

Barros

Costa-Silva

Biolchini

et al. 2009

Braz J Med Biol Res

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Evaluation of the prophylactic and therapeutic efficacies of mucus and tissue nucleoproteins extracted from Biomphalaria alexandrina snails on schistosomiasis mansoni

Nafie,

Abou-Gamra,

Mossalem

et al. 2024

J Parasit Dis

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A high-fat diet associated with acute schistosomiasis mansoni causes disorganization in splenic architecture in mice

Silva¹,

Corrêa

Neves

et al. 2012

Experimental Parasitology

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To date, the effect of the changes promoted by hypercholesterolemia and experimental schistosomiasis infection on splenic architecture has remained elusive. In this paper, we compared spleen from control and infected mice fed either high-fat (29% lipids) or standard diet (12% lipids), assessing spleen volume by liquid displacement and splenic disorganization by histopathology, morphometry and stereology. Infected mice showed higher spleen volume than in corresponding uninfected mice (P<0.05). The white pulp compartment was reduced, red pulp and germinal center were enhanced (P<0.01). Microscopic examination showed cellular infiltrates characterized by polymorfonuclear cells, with intensive lymphocytic mitosis and Mott cells. Hemosiderin deposits tended to be in less extent in infected mice compared with uninfected controls. The red pulp compartment showed a significantly (P<0.05) increased average number of megakaryocytes compared with uninfected mice, which may be associated with hematopoietic reconstitution. High-fat fed mice showed larger white pulp than controls (P<0.05). Standard fed mice showed exudative-productive granuloma distributed only sparsely in the red pulp, whereas a tissue reaction characterized by a cell infiltration in high-fat fed mice was found. The results of the present study suggest that there is a significant relationship between high-fat diet intake and splenic disorganization such as a decrease in the numerical density of white pulp and, red pulp and germinal center hyperplasia. Such structural disorganization due to co-morbidites (schistosomiasis and dyslipidemia) may affect the microenvironments of the spleen that are necessary for the generation of immune responses to antigens.

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Hepatic stereology of schistosomiasis mansoni infected-mice fed a high-fat diet

Cited by 27 publications

References 44 publications

Effect of praziquantel administration on hepatic stereology of mice infected with Schistosoma mansoni and fed a low-protein diet

Effect of praziquantel administration on hepatic stereology of mice infected with Schistosoma mansoni and fed a low-protein diet

Evaluation of the prophylactic and therapeutic efficacies of mucus and tissue nucleoproteins extracted from Biomphalaria alexandrina snails on schistosomiasis mansoni

A high-fat diet associated with acute schistosomiasis mansoni causes disorganization in splenic architecture in mice

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