Hepatitis C virus genotype testing in paraffin wax embedded liver biopsies for specimen identification

Ikura, Y

doi:10.1136/jcp.56.12.960

Cited by 2 publications

(2 citation statements)

References 7 publications

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“…In such cases, the biopsy specimens were mixed up during sample transfer or tissue processing. RNA extracted from the paraffin‐embedded liver specimens was examined by a PCR‐based HCV genotype assay, 7,8 enabling correct identification of the specimens. However, there is no method to distinguish specimens from a single patient that have become mixed up.…”

Section: Discussionmentioning

confidence: 99%

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New Type of Cassette for Biopsy Samples

Okada

Abe

Kawai

et al. 2007

Digestive Endoscopy

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Background: Biopsy samples may be erroneously mixed up during endoscopic examination. Although the conventional cassette which we previously reported was opaque before the fixation, it was not hard to confirm biopsy specimens in the cassette after closing the cover because the cover of conventional cassettes used resin which was not resistant against alcohol and xylene, resulting in non-transparent resin. Materials: We developed a new safety cassette for biopsy samples with a transparent cover in collaboration with ANQ (ANQ W-Bio TM ; ANQ, Ashiya, Japan), and also developed an original plate that allows confirmation of the number of biopsy specimens. Results: Using the new cassettes, we can easily confirm the presence and numbers of biopsy specimens without opening the cover. It is not necessary to open the cover until we fix the biopsy specimens with paraffin. With this newly developed cassette, one can prevent the loss of biopsy specimens, as well as mix-ups, facilitating work efficiency. Conclusion: We developed the new cassette for biopsy samples during endoscopic examination. With the cassette, we were able to confirm the number of biopsy specimens, and reconfirm them at each phase of processing. This new type of cassette for biopsy samples is useful and convenient in comparison to the conventional cassette.

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Section: Discussionmentioning

confidence: 99%

“…Immunohistochemistry for ABO blood group antigen is the most frequently used method. Sex chromosomes, HLA antigens, DNA genotyping, and microsatellite PCR can also be used for specimen identification 5–8 . However, these methods need a relatively long period and it is impossible to do these procedures in all hospitals.…”

Section: Introductionmentioning

confidence: 99%