Hepatitis C Virus Genotypes in Blood Donors and Patients with Chronic Hepatitis C

Brojer, Ewa; Głoskowska-Moraczewska, Z; Kacperska, E; Medyńska, Joanna; Cianciara, J; Juszczyk, Jacek; Loch, T; Flieger, J.; Seyfried, H

doi:10.1046/j.1423-0410.1996.7110051.x

Cited by 3 publications

(1 citation statement)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Poland, about 90 % of patients are infected by the particularly dangerous HCV-1b subspecies (Brojer et al 1996 ). In case of infection with this virus, only 46 % of patients can be completely cured (Brojer et al 1996 ), while in patients infected with the virus of other genotypes, PEG-IFNα/RBV therapy is effective in 77 % of cases (Fried et al 2002 ; Manns et al 2001 ).…”

Section: Introductionmentioning

confidence: 99%

Identification of mutations in the HVR1 and PKR-BD regions in HCV-infected patients resistant to PEG-IFNα/RBV therapy

et al. 2015

View full text Add to dashboard Cite

The identification of mutations in the HVR1 region of hepatitis type C virus (HCV) is time-consuming and expensive, and there is a need for a rapid, inexpensive method of screening for these mutations to predict the ineffectiveness of pegylated interferon alpha combined with ribavirin (PEG-IFNα/RBV) therapy. The project was designed to evaluate the usefulness of the high resolution melting (HRM) technique to screen for mutation in the cDNAs encoding the HVR1 and protein kinase R-binding domain (PKR-BD) regions in a group of 36 patients infected with HCV and resistant to 12 months of combined therapy with PEG-IFNα/RBV. Viral RNA was isolated, reverse transcribed, and the fragments encoding the HVR1 and PKR-BD regions were polymerase chain reaction (PCR)-amplified, cloned, sequenced, and the melting profiles and the melting temperature (Tm) were determined by the HRM technique. After the treatment, the melting profiles of HVR1 cDNAs revealed a dominant peak corresponding to the Tm of about 85 °C (HCVs85) in almost all patients. One or more minor peaks were also observed, indicating the existence of cDNA(s) of different Tm. The HMR analysis suggested four typical forms of response to treatment. These suppositions were supported by sequencing. The HRM analysis revealed no changes in the melting profiles of PKR-BD cDNAs in the same patient before and after the therapy, suggesting that, within 12 months of treatment, new mutations were not introduced in PKR-BD. These findings were substantiated by sequencing. The HRM technique can be applied for the rapid screening for mutations in the cDNAs encoding the HVR and PKR-BD regions of HCV. We suggest that the detection of HCVs85 peak before the IFNα/RBV therapy might predict the ineffectiveness of treatment.

show abstract

Section: Introductionmentioning

confidence: 99%

Identification of mutations in the HVR1 and PKR-BD regions in HCV-infected patients resistant to PEG-IFNα/RBV therapy

et al. 2015

View full text Add to dashboard Cite

show abstract

Hepatitis C virus genotypes in hemophiliacs in the state of Minas Gerais, Brazil

et al. 1999

View full text Add to dashboard Cite

Although the frequency of genotypes observed (types 1 and 3) among hemophiliacs in the state of Minas Gerais was higher than that in the southern part of the country, these frequencies were not different from those in other groups of patients in Brazil and other countries studied. Further investigation is needed of the evidence that the type 3 isolates observed in these studies are significantly different from other isolates previously characterized by sequence analysis.

show abstract

The hepatitis C virus genotype and subtype frequency in hepatitis C virus RNA‐positive, hepatitis C virus antibody‐negative blood donors identified in the nucleic acid test screening program in Poland

et al. 2004

View full text Add to dashboard Cite

show abstract

Hepatitis C Virus Genotypes in Blood Donors and Patients with Chronic Hepatitis C

Cited by 3 publications

References 24 publications

Identification of mutations in the HVR1 and PKR-BD regions in HCV-infected patients resistant to PEG-IFNα/RBV therapy

Identification of mutations in the HVR1 and PKR-BD regions in HCV-infected patients resistant to PEG-IFNα/RBV therapy

Hepatitis C virus genotypes in hemophiliacs in the state of Minas Gerais, Brazil

The hepatitis C virus genotype and subtype frequency in hepatitis C virus RNA‐positive, hepatitis C virus antibody‐negative blood donors identified in the nucleic acid test screening program in Poland

Contact Info

Product

Resources

About