1997
DOI: 10.1016/s0168-8278(97)80137-7
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Hepatitis C virus (HCV) genotypes in Spanish patients with HCV infection: relationship between HCV genotype 1b, cirrhosis and hepatocellular carcinoma

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Cited by 108 publications
(85 citation statements)
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“…HCV genotype was determined by restriction fragment length polymorphism after amplification of the 5Ј noncoding region of the HCV genome, as described previously. 20 If HCV RNA was undetectable, HCV genotype was determined with a serotyping assay that detects type-specific antibodies directed to epitopes encoded by the NS4 region of the genome (Murex HCV Serotyping; Abbot Cientifica S.A., Madrid, Spain). The assay identifies the HCV type (1-6) with high accuracy but does not provide information on the HCV subtype.…”
Section: Methodsmentioning
confidence: 99%
“…HCV genotype was determined by restriction fragment length polymorphism after amplification of the 5Ј noncoding region of the HCV genome, as described previously. 20 If HCV RNA was undetectable, HCV genotype was determined with a serotyping assay that detects type-specific antibodies directed to epitopes encoded by the NS4 region of the genome (Murex HCV Serotyping; Abbot Cientifica S.A., Madrid, Spain). The assay identifies the HCV type (1-6) with high accuracy but does not provide information on the HCV subtype.…”
Section: Methodsmentioning
confidence: 99%
“…HCV genotype was determined by restriction fragment length polymorphism (RFLP) analysis of the 5ЈNCR as previously described. 23,24 HCV-RNA concentration in serum was measured by the COBAS Amplicor HCV Monitor test, v2.0 (Roche Diagnostic Systems, Inc., Branchburg, NJ).…”
Section: Methodsmentioning
confidence: 99%
“…HCV genotyping was performed by restriction fragment length polymorphism (RFLP) analysis of the 5Ј'NCR, basically as described. 25,27 Amplification of the NS3 Region and DNA Sequencing A segment of the NS3 region encompassing nucleotides 4429 to 4719 (positions referred to the HCV-1 prototype), which includes HLA-A2-restricted (KLVALGINAV) and HLA-A3-restricted (LIFCHSKKK) T-cell epitopes, was amplified by nested PCR. Several studies indicate that HCV Class I restricted T-cell epitopes located within the NS3 protein are commonly recognized by liver-infiltrating and circulating cytotoxic T lymphocytes (CTL) of HCV-infected patients.…”
Section: Hcv-rna Viral Load and Genotypingmentioning
confidence: 99%