Hepatitis C virus variants from Vietnam are classifiable into the seventh, eighth, and ninth major genetic groups.

Tokita, Hajime; Okamoto, Hiroaki; Tsuda, Fumio; Song, Pham; Nakata, Susumu; Chosa, Toru; Iizuka, Hisao; Mishiro, Shunji; Miyakawa, Yuzo; Mayumi, Makoto

doi:10.1073/pnas.91.23.11022

Cited by 167 publications

(146 citation statements)

References 24 publications

Supporting

Mentioning

141

Contrasting

Order By: Relevance

“…Genetic variants of HCV are known to be widely spread around the world. Genotypes 1, 2 and 3 are found on all continents, but in some geographical areas, such as Africa and Southeast Asia, viral isolates are highly divergent and particular genotypes or subtypes are predominant (13)(14)(15)(16). These data suggest the existence of a long-term endemic infection in these areas and some investigators have hypothesized that HCV might have originated in such places (17).…”

Section: Discussionmentioning

confidence: 87%

Geographic distribution of hepatitis C virus genotypes in Brazil

Campiotto

Pinho

Carrilho

et al. 2005

Braz J Med Biol Res

174

102

View full text Add to dashboard Cite

Brazil is a country of continental dimension with a population of different ethnic backgrounds. Thus, a wide variation in the frequencies of hepatitis C virus (HCV) genotypes is expected to occur. To address this point, 1,688 sequential samples from chronic HCV patients were analyzed. HCV-RNA was amplified by the RT-PCR from blood samples collected from 1995 to 2000 at different laboratories located in different cities from all Brazilian States. Samples were collected in tubes containing a gel separator, centrifuged in the site of collection and sent by express mail in a refrigerated container to Laboratório Bioquímico Jardim Paulista, São Paulo, SP, Brazil. HCV-RNA was extracted from serum and submitted to RT and nested PCR using standard procedures. Nested PCR products were submitted to cycle sequencing reactions without prior purification. Sequences were analyzed for genotype determination and the following frequencies were found: 64.9% (1,095) for genotype 1, 4.6% (78) for genotype 2, 30.2% (510) for genotype 3, 0.2% (3) for genotype 4, and 0.1% (2) for genotype 5. The frequencies of HCV genotypes were statistically different among Brazilian regions (P = 0.00017). In all regions, genotype 1 was the most frequent (51.7 to 74.1%), reaching the highest value in the North; genotype 2 was more prevalent in the Center-West region (11.4%), especially in Mato Grosso State (25.8%), while genotype 3 was more common in the South (43.2%). Genotypes 4 and 5 were rarely found and only in the Southeast, in São Paulo State. The present data indicate the need for careful epidemiological surveys throughout Brazil since knowing the frequency and distribution of the genotypes would provide key information for understanding the spread of HCV.

show abstract

Section: Discussionmentioning

confidence: 87%

Geographic distribution of hepatitis C virus genotypes in Brazil

Campiotto

Pinho

Carrilho

et al. 2005

Braz J Med Biol Res

174

102

View full text Add to dashboard Cite

show abstract

“…Other type-specific polymorphisms in this region occur in regions predicted to be unpaired (positions -331, -330, -329, -313, -308, -307, -305 and -284) or strengthen proposed stem structures (-271, -264 and -279). The direct repeats of CACUCC at positions -319 and -304 noted previously (Takamizawa et al, 1991) occur in all sequences including this region, and are also reported for sequences of type 6 and other novel types from Vietnam (Tokita et al, 1994a).…”

Section: Structural Implications Of 5"ncr Sequence Variationmentioning

confidence: 82%

“…Furthermore, the accuracy of the LiPA could be increased by designing primers to recognize types 3b, 5 and 6. Neither typing system would distinguish all virus subtypes, or some novel genotypes discovered in Vietnam with 5'NCR sequences similar to type 1 viruses (Tokita et al, 1994a), but from previous extensive surveys these virus types are probably of limited distribution (Bukh et al, 1993;Simmonds et al, 1993 a). Finally, the range of virus types and subtypes present in any one geographical region is generally limited Davidson et al, 1995), and so the task of distinguishing between virus types in any one country would therefore be simpler than for the world wide collection of sequences analysed here.…”

Section: Accuracy Of Methods Using 5'ncr Polymorphisms For Virus Genomentioning

confidence: 99%

“…Type 3a viruses have several substitutions and a deletion in this region, but maintain a stem of 6 base pairs (Fig. 6), while for several novel genotypes from Vietnam (Tokita et al, 1994a) the stem is reduced to 5 base pairs. This suggests an important role for this structure, perhaps in the replication of virus RNA, since this loop appears dispensable for IRES function in some studies (Tsukiyama Kohara et al, 1992;Wang et al, 1993) although not in others (Fukushi et al, 1994).…”

Section: Structural Implications Of 5"ncr Sequence Variationmentioning

confidence: 99%

See 1 more Smart Citation

Variation of the hepatitis C virus 5' non-coding region: implications for secondary structure, virus detection and typing

Smith

Mellor²,

Jarvis³

et al. 1995

Journal of General Virology

220

160

View full text Add to dashboard Cite

Variation in the 5' non-coding region (5'NCR) of hepatitis C virus (HCV) was investigated in detail by comparing 314 5'NCR sequences of viruses ofgenotypes 1 to 6. Evidence was obtained for the existence of associations between particular 5'NCR sequence motifs and virus types and subtypes. No recombination was observed between the 5'NCR and coding regions of different genotypes, implying that the sequence of subgenomic regions such as the 5'NCR can be used to deduce virus genotype. The distribution of polymorphic sites within the 5'NCR is used to propose improved oligonucleotide primers for virus detection and quantification that would be equally efficient in detecting RNA of different virus genotypes. The accuracy of two different genotyping methods (RFLP and the line probe assay) based on analysis of sequence polymorphisms in the 5'NCR is predicted from the sequences surveyed to be 97 % and 83 % respectively for types 1 to 6, with higher accuracies for distinguishing between subtypes la/lb, 2a/2b or 3a/3b. Several sites of genotype-specific polymorphism were covariant and maintained the base pairings required for a secondary structure model of the 5"NCR. Other sites of variation suggest minor modifications to this model and have implications for the probable functions of the 5"NCR

show abstract

“…The most common genotypes in Vietnam were genotypes 1 and 6, about 40% each, followed by genotypes 2 and 3, ranging from 5% to 17%. 11,[35][36][37][38][39][40] HIV and viral hepatitis coinfection. As a consequence of shared transmission route, coinfection of HIV and hepatitis B and C is common.…”

Section: Overview-hepatitis B and C And Hiv Coinfection In Vietnammentioning

confidence: 99%

Epidemiology, Responses, and Way Forward

Sereno

Mesquita²,

Kato³

et al. 2012

Journal of the International Association of Physicians in AIDS

View full text Add to dashboard Cite

Viral hepatitis is a disease of great concern to public health that is now met by a favorable momentum to combat the global epidemic. This article is intended to highlight the importance of viral hepatitis in the Vietnam population as well in the group of people living with HIV/AIDS (PLWHA). We reviewed available data on epidemiology and response on hepatitis B virus (HBV) and hepatitis C virus (HCV) and HIV coinfection in Vietnam. The hepatitis B surface antigen (HBsAg) prevalence in the general population ranged from 5.7% to 24.7%. The anti-HCV prevalence ranged from 0.38% to 4.3% in the general population, while among people who inject drugs (PWIDs) it ranged from 31% to 97.2%. The HBV prevalence among PLWHA is similar to the general population, while HCV/HIV coinfection is concentrated in some groups. Anti-HCV prevalence among HIV-infected PWIDs can be as high as 98.5%. Developing policies for diagnosis and treatment of chronic HBV and HCV infections are critical priorities in order to prevent clinical progression to cirrhosis and liver cancer.

show abstract

Hepatitis C virus variants from Vietnam are classifiable into the seventh, eighth, and ninth major genetic groups.

Abstract: Thirty-four (41%) of 83 hepatitis C virus (HCV) isolates from commercial blood donors in Vietnam were not classifiable into genotype I/la, H/lb, HI/2a, IV/2b, or

Cited by 167 publications

References 24 publications

Geographic distribution of hepatitis C virus genotypes in Brazil

Geographic distribution of hepatitis C virus genotypes in Brazil

Variation of the hepatitis C virus 5' non-coding region: implications for secondary structure, virus detection and typing

Epidemiology, Responses, and Way Forward

Contact Info

Product

Resources

About