Hepato-protective Effects of Crude Phenol and Petroleum Spirit Root Extracts of Calotropis procera (Sodom of Apple) on CCL4 Induced Hepatotoxicity in Albino Rats

Abstract

The efficiency of current synthetic agents in treating chronic liver disease is not satisfactory and they have undesirable side effects. The effects of crude phenol root extracts of Calotropis procera, petroleum spirit root extracts of Calotropis procera (PRECP, PSRECP) and livolin on liver function indices of ccl4 induced hepatotoxicity rats model was evaluated. Fifty (50) albino rats were grouped into Five (I, II, III, IV and V) of 10 rats each, 120mg/kg body weight ccl4 diluted with olive oil in the ratio 1:1 was administered to rats in groups II, III, IV and V intramuscularly followed by oral administration of 10mg/kg livolin, 10mg/kg, crude phenol and petroleum spirit root extracts of C. Procera to group III, IV and V respectively. Groups I and II serves as positive and test control respectively. Analysis of variance (ANOVA) for multiple comparison test were used to compare the indices of the liver and kidney functions for the test and control group at 10 days interval for 20 days. The hepatic biochemical markers Alanine Amino Transferase (ALT), Aspartate Amino Transferase (AST), Alkaline Phosphatases (ALP) of group Gp II were significantly higher (P<0.001) compared to gpi, while group III (treated with livolin) was statistically decreased (P<0.05) when compared with control (Gp I), this confirms the toxicity and treatment with livolin respectively. Oral administrations of the PRECP lowered all the liver function markers and increased the concentration of urea and albumin after 20 days of exposure. This indicates that PRECP may reverse the chemically induced tissue damage; in contrast, PSRECP produced toxicity at both exposures as evidenced from the histopathology of the liver hepatocytes. The histopathological analysis of PERCP indicates improved fine architecture of the liver and kidney cells which are comparable to livolin treated group. In conclusion, the overall results suggest that ethanol root extracts of C. Procera may have moderate hepatocurative effects when compared to methanol extracts.

Therapeutic potential of Ni(II) Schiff base complex on CCl4 toxicity

“…In this study, the CCl4 group displayed decreased ALB and TP as another symptom of the CCl4-persuaded deterioration to hepatic cell organelles involved in protein synthesis confirming limited liver synthetic function. A similar result was reported by Shehu et al [35]. Returning to the present study, rats subjected to CCl4 suffer from marked reductions in hepatic nucleic acid (DNA&RNA) content and hepatic protein level.…”