Hepatoblastoma with Squamous Differentiation in a B6C3F1 Mouse

Nonoyama, Takashi; Reznik, Gerd; Bucci, Thomas J.; Fullerton, Floyd R.

doi:10.1177/030098588602300511

Cited by 20 publications

(9 citation statements)

References 3 publications

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“…Embryonal cells may also exhibit metaplasia with resultant production of osteiod. 1 Hepatoblastomas have been described in sheep, 10 mice, 12,13 pigs, 5 a bull, 15 and a horse 14 and have been reported in both young and adult animals. Hepatoblastomas in mice may be either spontaneous or chemically induced.…”

Section: Hepatoblastoma In An Equine Fetusmentioning

confidence: 99%

Hepatoblastoma in an Equine Fetus

Neu

1993

J VET Diagn Invest

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Section: Hepatoblastoma In An Equine Fetusmentioning

confidence: 99%

Hepatoblastoma in an Equine Fetus

Neu

1993

J VET Diagn Invest

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“…In addition, the tumor cells in the present case also showed abundant tonofilaments. Tonofilaments are known to characterize epithelial features of tumor cells and to be present in tumor cells of HCC, CCC [9] and hepatoblastoma [8]. Vimentin and bile duct-type cytokeratin expressions were scattered in HCC components.…”

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confidence: 99%

Combined Hepatocellular and Cholangiocellular Carcinoma in a Dog.

2001

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ABSTRACT. A transitional type of combined hepatocellular and cholangiocellular carcinoma developed in a 12-year-old male Yorkshire terrier dog. The tumor was histologically composed of both hepatocellular carcinoma and cholangiocellular carcinoma components, and both elements were closely intermingled. Intraluminal mucin accumulation in cytokeratin-positive tubular/glandular structures was observed within the cholangiocellular carcinoma components and this feature was useful histological marker for a differential diagnosis between combined hepatocellular and cholangiocellular carcinoma and a pseudoglandular type of hepatocellular carcinoma. This primary hepatic tumor is extremely rare in dogs.

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“…Reported cases of HB in domestic animals are those in a horse [16], a bull [17], and dogs [24]. In laboratory animals, there are detailed descriptions on the HBs of mice [2,9,10]. However, a little pathological information on canine HBs has been available [24].…”

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“…As for the histogenesis of human HB, thus, small cell-embryonal-fetal cell line was suspected to be the most frequent course of differentiation [1,18]. On the other hand, cells from murine HB lacked immunohistochemically demonstrable AFP [2,9], and its histogenesis have not yet been established [9,10]. Frith et al [3] speculated that murine HB are probably either of a liver stem cell or ductule origin.…”

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Hepatoblastoma in a Dog.

et al. 1997

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ABSTRACT. A hepatoblastoma was found in a 13-year-old female Maltese dog. Histologically, the tumor showed a wide trabecular pattern and was frequently accompanied with vascular lake formation. Tumor cells were positive for cytokeratin and neuron specific enolase, but negative for chromogranin. Electronmicroscopically, tumor cells were accompanied with continuous basement membrane and had poorly developed desmosomes. Sinusoidal endothelia had fenestration and were surrounded by myofibroblast-like cells. To the best of our knowledge, this paper is the first report of morphological studies on canine hepatoblastoma. -KEY WORDS: canine, hepatoblastoma.J. Vet. Med. Sci. 59(12): 1167-1170, 1997 periodic acid-Schiff (PAS) reaction with or without diastase digestion, Orcein stain, and Watanabe's silver impregnation.Mitoses of tumor cells were counted and the data were compared with those of a canine hepatocellular carcinoma with distant metastasis.Immunohistochemical examination was performed using labelled streptavidin-biotin method with a Histofine kit (Nichirei Corp., Tokyo, Japan). Primary antibodies used were as follows: anti-human cytokeratin antibody (Boehringer Mannheim Biochemica, Mannheim, Germany), anti-human neuron specific enolase (NSE) antibody (Dakopatts, Glostrup, Denmark), anti-bovine chromogranin antibody (Incstar Corp., Stillwater, MN, U.S.A.), anti-α-smooth muscle actin (α-SMA) antibody (Sigma Chemical Co., St. Louis, MO, U.S.A.), and anti-human factor VIIIrelated antigen (FVIII-RAg) antibody (Dakopatts, Glostrup, Denmark). Immunoreaction with primary antibodies was performed for 1 hr at 37°C. Thereafter, biotinylated antirabbit or mouse IgG antibody and peroxidase-labelled streptavidin were sequentially applied to the sections for 15 min each at room temperature. The reaction was visualized with 3,3'-diaminobenzidine in 0.1M Tris HCl buffer (pH 7.6) plus hydrogen peroxide. Sections were counterstained with Mayer's hematoxylin.For electron microscopy, small blocks (1 mm 3 ) of the tumor were fixed in 2.5% glutaraldehyde/0.1 M phosphate buffer for 2 hr at 4°C and post-fixed in 1% osmium tetroxide/0.2 M phosphate buffer for 2 hr at 4°C. The blocks were dehydrated in graded ethanol and embedded in Epoxy resin. Ultra-thin sections were cut and double stained with uranyl acetate and lead citrate and examined by a Hitachi H-300 transmission electron microscope at 75 kV.Histologically, the nodules were consisted of compact proliferation of tumor cells in a wide trabecular pattern (Fig. 1). Many vascular lakes were present in the tumor tissues (Fig. 1). Necrosis and degeneration were rare. However, in the central portions of tumor nests, neoplastic cells fell into degeneration (Fig. 2a) and these portions occasionally connected to the vessels in the stroma (Fig. 2b). The tumor cells had a polarity: their nuclei were located at the opposite site of basal side (Fig. 2a). The nuclei of the tumor cells were round with relatively rich chromatin and did not show atypia or pleomorphism. The nucleus/cytoplasm ratio...

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Hepatoblastoma with Squamous Differentiation in a B6C3F1 Mouse

Cited by 20 publications

References 3 publications

Hepatoblastoma in an Equine Fetus

Hepatoblastoma in an Equine Fetus

Combined Hepatocellular and Cholangiocellular Carcinoma in a Dog.

Hepatoblastoma in a Dog.

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