Hepatocellular carcinoma – time to take the ticket

Mullath, Anju; Krishna, Murali

doi:10.4240/wjgs.v11.i6.287

Cited by 7 publications

(6 citation statements)

References 42 publications

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“…Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related death worldwide, after lung cancer and gastric cancer (1). HCC treatment has evolved rapidly, but recurrence rate and prognostic outcome are not promising (2,3), and 5-year survival rate is discouraging (4,5). Therefore, it is imperative to dissect HCC internal molecular mechanism, which is expected to contribute to HCC patients' treatment.…”

Section: Introductionmentioning

confidence: 99%

Hsa_circ_0097009 regulates proliferation, apoptosis, migration and invasion of hepatocellular carcinoma cells via miR-568/RNF38 axis

Wu¹,

Li²,

Liu³

et al. 2023

BLL

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BACKGROUND: Circular RNAs (circRNAs), a class of non-coding RNAs, has been proved as an essential driver of hepatocellular carcinoma (HCC) progression. However, the aim of this study is to explore the role of hsa_circ_0097009 (circ_0097009) in HCC progression. METHODS: Ki67 expression in HCC tissues was labeled and examined by IHC assay. Quantitative real-time PCR (qRT-PCR) was applied to assess the expression of circ_0097009, miR-568 and RING fi nger protein 38 (RNF38). Western blot assay was conducted to analyze protein expression. HCC cell colony formation, proliferation, migration, invasion, angiogenesis ability and apoptosis were determined by colony formation assay, 5-ethynyl-29-deoxyuridine (EdU) assay, wound healing assay, trans well assay, angiogenesis assay and fl ow cytometry. The interaction between circ_0097009 and miR-568 as well as miR-568 and RNF38 was probed by dual-luciferase reporter and RNA immunoprecipitation (RIP) assays. RESULTS: We found that circ_0097009 and RNF38 expressions were markedly higher, but miR-568 expression was signifi cantly lower in HCC. Circ_0097009 knockdown blocked HCC cell cloning, proliferation, migration, invasion, angiogenesis and facilitated apoptosis. MiR-568 was a target of circ_0097009 in HCC cells. MiR-568 interference could attenuate the circ_0097009 knockdown-induced inhibition on HCC cells progress. MiR-568 depressed cell clonal formation, proliferation, migration, invasion, angiogenesis and promotion of apoptosis, by targeting RNF38, and RNF38 overexpression reversed the suppression of miR-568 in HCC cells. Also, circ_0097009 knockdown blocked tumor growth in vivo CONCLUSION: Together, this study indicated that the oncogenic function of circ_0097009 in HCC, and circ_0097009/miR-568/RNF38 axis was expected to be a novel therapeutic option for HCC patients (Tab. 1, Fig. 7, Ref. 33).

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Section: Introductionmentioning

confidence: 99%

Hsa_circ_0097009 regulates proliferation, apoptosis, migration and invasion of hepatocellular carcinoma cells via miR-568/RNF38 axis

Wu¹,

Li²,

Liu³

et al. 2023

BLL

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“…Hepatocellular carcinoma (HCC) is the main histological subtype of primary liver cancer with rapidly increasing morbidity and mortality, especially in China [ 1 ]. Although many patients are effectively diagnosed and treated in the early stage of the disease, the recurrence rate is still very high [ 2 , 3 ]. And the prognosis of HCC is not optimistic, especially for patients with advanced clinical stage, when tumor spreads to the surrounding lymph nodes, the 5-year survival rate of patients is only 11%; when cancer spreads to some important organs, the situation is worse, with a 5-year survival rate of only 3% [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

A novel circular RNA circ-LRIG3 facilitates the malignant progression of hepatocellular carcinoma by modulating the EZH2/STAT3 signaling

Sun

Gao

Zhou

et al. 2020

J Exp Clin Cancer Res

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Background Circular RNA (circRNA) is emerging as an important player in human diseases, especially cancer. In our previous study, we identified a series of deregulated circRNAs in hepatocellular carcinoma (HCC) by performing circRNA microarray expression profile. Here, we aimed to explore the role of circ-LRIG3 (hsa_circ_0027345) in HCC. Methods qRT-PCR and western blot were used to asses gene and protein expression, respectively. CCK-8, EdU and Transwell assays were used to detect cell proliferation, migration and invasion. GSEA software was applied to analyze the pathway related to circ-LRIG3. Co-IP, RIP and ChIP assays were used to identify the positive feedback axis of circ-LRIG3/EZH2/STAT3. Animal study was carried to test the role of circ-LRIG3 in vivo. Results Circ-LRIG3 was notably upregulated in HCC and promoted HCC cell proliferation, migration, invasion and reduced apoptosis. Circ-LRIG3 formed a ternary complex with EZH2 and STAT3, facilitating EZH2-induced STAT3 methylation and subsequent phosphorylation, resulting in the activation of STAT3 signaling. In turn, activated STAT3 could directly bind to circ-LRIG3 promoter to increase circ-LRIG3 transcription activity, thus forming a positive feedback loop. The animal models showed that exogenous expression of circ-LRIG3 enhanced tumorigenicity and metastasis in vivo, whereas these effects were blocked after treatment with C188–9, a specific STAT3 small-molecule inhibitor. Clinically, high circ-LRIG3 was closely linked with aggressive clinicopathological features and was identified as an independent risk prognostic factor of overall survival. Importantly, plasma circ-LRIG3 was found to be a highly sensitive and specific non-invasive diagnostic indicator for HCC. Conclusions Our study reveals the carcinogenic role of circ-LRIG3 in HCC, which may provide a new therapeutic target for HCC patients.

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“…A number of staging and grading systems have been commonly used for LIHC, including TNM, Barcelona Clinic Liver Cancer (BCLC), albuminbilirubin (ALBI) grading, and Okuda system (Selcuk, 2017). For LIHC patients, surgical excision is an optimal treatment (Mullath and Krishna, 2019). However, the majority of LIHC patients have the risks of recurrence and metastasis, leading to a much lower 5 years survival (Buonaguro et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

OSlihc: An Online Prognostic Biomarker Analysis Tool for Hepatocellular Carcinoma

Wang

Zhang

et al. 2020

Front. Pharmacol.

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Liver hepatocellular carcinoma (LIHC) is one of the most common malignant tumors in the world with an increasing number of fatalities. Identification of novel prognosis biomarker for LIHC may improve treatment and therefore patient outcomes. The availability of public gene expression profiling data offers the opportunity to discover prognosis biomarkers for LIHC. We developed an online consensus survival analysis tool named OSlihc using gene expression profiling and long-term follow-up data to identify new prognosis biomarkers. OSlihc consists of 637 cases from four independent cohorts. As a risk assessment tool, OSlihc generates the Kaplan-Meier survival plot with hazard ratio (HR) and p value to evaluate the prognostic value of a gene of interest. To test the reliability of OSlihc, we analyzed 65 previous reported prognostic biomarkers in OSlihc and showed that all of which have significant prognostic values. Furthermore, we identified four novel potential prognostic biomarkers (ATG9A, WIPI1, CXCL1, and CSNK2A2) for LIHC, the elevated expression of which predict the unfavorable survival outcomes. These genes (ATG9A, WIPI1, CXCL1, and CSNK2A2) may be potentially new biomarkers to identify at-risk LIHC patients when further validated. By OSlihc, users can evaluate the prognostic abilities of genes of their interest, which provides a platform for researchers to identify prognostic biomarkers to further develop targeted therapy strategies for LIHC patients. OSlihc is public and free to the users at http://bioinfo.henu.edu.cn/LIHC/LIHCList.jsp.

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Hepatocellular carcinoma – time to take the ticket

Cited by 7 publications

References 42 publications

Hsa_circ_0097009 regulates proliferation, apoptosis, migration and invasion of hepatocellular carcinoma cells via miR-568/RNF38 axis

Hsa_circ_0097009 regulates proliferation, apoptosis, migration and invasion of hepatocellular carcinoma cells via miR-568/RNF38 axis

A novel circular RNA circ-LRIG3 facilitates the malignant progression of hepatocellular carcinoma by modulating the EZH2/STAT3 signaling

OSlihc: An Online Prognostic Biomarker Analysis Tool for Hepatocellular Carcinoma

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