Hepatocyte growth factor in gingival crevicular fluid and the distribution of hepatocyte growth factor-activator in gingival tissue from adult periodontitis

Kakimoto, Kyoko; Machigashira, Miho; Ohnishi, Tomokazu; Kajihara, Takeshi; Semba, Ichiro; Setoguchi, Takashi; Tamura, Masato; Izumi, Yuichi; Daikuhara, Yasushi

doi:10.1016/s0003-9969(02)00050-x

Cited by 20 publications

(15 citation statements)

References 46 publications

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“…In 2002, Japanese authors found significant correlations between the salivary level of HGF and periodontal parameters such as PD and GI, and postulated that salivary HGF might be useful as a screening marker for early detection of periodontal disease (8). At the same time, some of the above ex vivo and clinical data (4)(5)(6)(7)(8) were confirmed by Daikuhara and coworkers (9,10). Notably, a recent study has shown that HGF overproduction by inflamed human gingival fibroblasts is mediated by protease-activated receptors (17), thus corroborating the HGF/periodontal disease relationship on a molecular basis.…”

Section: Discussionmentioning

confidence: 88%

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Hepatocyte growth factor in saliva is a potential marker of symptomatic periodontal disease

Wilczyńska-Borawska¹,

Borawski

Kovalchuk³

et al. 2006

J Oral Sci

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Evidence has been emerging that hepatocyte growth factor (HGF) -a pluripotential regenerative cytokine -is a key factor in the pathogenesis and progression of periodontal disease, mostly through its over-stimulation of gingival epithelial cell growth and impairment of the regeneration of collagenous structures. We measured the levels of immunoreactive HGF in unstimulated whole mixed saliva from 26 patients referred for treatment of periodontal disease, and from 20 healthy subjects. HGF was detected in all saliva samples from the patients, the concentration ranging from 0.06 to 5.38 ng/ml, with a mean concentration of 1.87 ± 1.32 ng/ml. In healthy individuals, the median salivary HGF level was 0.68 ng/ml (range: 0 -7.33 ng/ml), being almost 3-fold lower (P < 0.0001) than that in the patients. Periodontal parameters in the patients were: gingival index (

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Section: Discussionmentioning

confidence: 88%

“…Remarkably, HGF is also involved in the development of periodontal disease (3)(4)(5)(6)(7)(8)(9)(10). Recently, it was shown that HGF levels in unstimulated whole mixed saliva are directly correlated with probing depth and the percentage of sites positive for bleeding on probing in the general population (8).…”

Section: Introductionmentioning

confidence: 99%

Hepatocyte growth factor in saliva is a potential marker of symptomatic periodontal disease

Wilczyńska-Borawska¹,

Borawski

Kovalchuk³

et al. 2006

J Oral Sci

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“…Therefore, it is noteworthy that HGF was remarkably induced in human gingival fibroblasts by fimbriae and gingipains, both of which were representative virulence factors peculiar to putative periodontopathogenic P. gingivalis. In this context, HGF levels in gingival crevicular fluid also increase with periodontitis (55,56), and the HGF concentration in gingival crevicular fluid from healthy gingival tissue is ϳ2 ng/ml (55), ϳ10-fold that in serum from healthy subjects. These data indicate that HGF is actually produced in various tissues, including healthy gingival tissue.…”

Section: Discussionmentioning

confidence: 99%

“…These data indicate that HGF is actually produced in various tissues, including healthy gingival tissue. Furthermore, clinical data show that various diseases, including inflammation, degeneration, and oncogenesis, cause the augmentation of HGF levels in human body fluids (55,(57)(58)(59)(60)(61)(62)(63).…”

Section: Discussionmentioning

confidence: 99%

Arginine-Specific Gingipains from Porphyromonas gingivalis Stimulate Production of Hepatocyte Growth Factor (Scatter Factor) through Protease-Activated Receptors in Human Gingival Fibroblasts in Culture

Uehara

Muramoto

Imamura

et al. 2005

The Journal of Immunology

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Cystein proteinases (gingipains) from Porphyromonas gingivalis cleave a broad range of in-host proteins and are considered to be key virulence factors in the onset and development of adult periodontitis and host defense evasion. In periodontitis, an inflammatory disease triggered by bacterial infection, the production of hepatocyte growth factor (HGF) is induced not only by various factors derived from the host, such as inflammatory cytokines, but also by bacterial components. In this study we examined the possible enhanced production of HGF produced by human gingival fibroblasts upon stimulation with gingipains. Arginine-specific gingipain (Rgp) caused a marked production of HGF into the supernatant, the induction of HGF expression on the cell surface, and the up-regulation of HGF mRNA expression in a dose-dependent and an enzymatic activity-dependent manner. Because it has been reported that Rgp activated protease-activated receptors (PARs), we examined whether the induction of HGF triggered by Rgps on human gingival fibroblasts occurred through PARs. An RNA interference assay targeted to PAR-1 and PAR-2 mRNA revealed that gingipains-induced secretion of HGF was significantly inhibited by RNA interference targeted to PAR-1 and PAR-2. In addition, the Rgps-mediated HGF induction was completely inhibited by the inhibition of phospholipase C and was clearly inhibited by RNA interference targeted to p65, which is an NF-κB component. These results suggest that Rgps activated human gingival fibroblasts to secrete HGF in the inflamed sites and the mechanism(s) involved may actively participate in both inflammatory and reparative processes in periodontal diseases.

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“…These observations suggested that HGF may increase in the gingiva of patients with periodontitis. The presence of HGF in gingival crevicular fluid (GCF) was noted in previous studies; 15,16 however, the levels of HGF in health and disease and after treatment have not been studied in an Indian population.…”

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confidence: 98%

Hepatocyte Growth Factor Levels in Gingival Crevicular Fluid in Health, Disease, and After Treatment

Nagaraja

Pradeep

2007

Journal of Periodontology

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HGF concentration increased proportionally with the progression of periodontal disease, and HGF concentrations showed a positive correlation with the clinical parameters, suggesting that HGF plays a key role in periodontal disease progression. Also, following non-surgical periodontal therapy, the levels of HGF decrease significantly suggesting that HGF could be useful for monitoring the response to periodontal therapy.

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Hepatocyte growth factor in gingival crevicular fluid and the distribution of hepatocyte growth factor-activator in gingival tissue from adult periodontitis

Cited by 20 publications

References 46 publications

Hepatocyte growth factor in saliva is a potential marker of symptomatic periodontal disease

Hepatocyte growth factor in saliva is a potential marker of symptomatic periodontal disease

Arginine-Specific Gingipains from Porphyromonas gingivalis Stimulate Production of Hepatocyte Growth Factor (Scatter Factor) through Protease-Activated Receptors in Human Gingival Fibroblasts in Culture

Hepatocyte Growth Factor Levels in Gingival Crevicular Fluid in Health, Disease, and After Treatment

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