Hepatoprotective activity of<i>Glycosmis pentaphylla</i>against paracetamol-induced hepatotoxicity in Swiss albino mice

Nayak, Siva Shankar; Jain, Ranjeet; Sahoo, Atish Kumar

doi:10.3109/13880209.2010.501084

Cited by 49 publications

(39 citation statements)

References 12 publications

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“…From ancient times, man has always used herbs for various liver disorders as a treatment strategy, because natural remedies from traditional medicinal plants have proven to be effective alternative treatments in cases of liver injury. From various studies, it has been found that hepatoprotective effects are directly associated with phytoconstituents (Sabir and Rocha, 2008;Choi et al, 2009;Bhaskar and Balakrishnan, 2010;Huang et al, 2010;Nayak et al, 2011;Rehman et al, 2012;Ibrahim et al, 2013). All around the world, and especially in developing countries, people use traditional herbal medicines; therefore, there is a strong need to develop such a hepatoprotective medicine using natural products that can pass the safety evaluation and screening in the early phase of drug discovery because most toxic compounds are metabolized in the liver (Iwalokun et al, 2006;Adeneye, 2009;Ajiboye et al, 2010;Ahmed et al, 2013;Chanda et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Hepatoprotective effects of Malva sylvestris L. against paracetamol-induced hepatotoxicity

Hussain¹,

Ikram²,

Rehman³

et al. 2014

Turk J Biol

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Malva sylvestris is traditionally used for the treatment of liver diseases, but sufficient pharmacological-based scientific literature is not available online to authenticate its use in liver ailments. We aimed to assess the hepatoprotective effects of Malva sylvestris against paracetamol-induced hepatotoxicity in mice. The extract was concentrated using a rotary evaporator and then desired concentrations of extracts were made by dissolving in normal saline. The standard drug silymarin (100 mg/kg) was used as a reference drug to compare the therapeutic effects of Malva sylvestris. Two different doses of Malva sylvestris (300 and 600 mg/kg) were administered intraperitoneally for 7 consecutive days followed by intraperitoneal administration of paracetamol (250 mg/kg). Paracetamol significantly induced oxidative stress in the liver, ultimately leading to increased serum levels of liver enzyme markers like alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total bilirubin, and direct bilirubin. The extract of Malva sylvestris significantly reduced the serum levels of these elevated liver enzyme markers in a dose-dependent manner. Histopathological examination of liver tissues also showed hepatoprotective effects of Malva sylvestris in restoring normal functional ability of the liver. The results of our study strongly suggest that the extract of Malva sylvestris has strong hepatoprotective effects against paracetamol-induced liver injury, thereby scientifically affirming its traditional therapeutic role in liver injury.

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Section: Introductionmentioning

confidence: 99%

Hepatoprotective effects of Malva sylvestris L. against paracetamol-induced hepatotoxicity

Hussain¹,

Ikram²,

Rehman³

et al. 2014

Turk J Biol

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“…A substantial number of herbal formulations have been affirmed to have medicinal properties to cure several life-threatening diseases (Sabir and Rocha, 2008;Akash et al, 2011;Akash et al, 2014a,b;Bhaskar and Balakrishnan, 2010;Huang et al, 2010;Venkatesh et al, 2010;Nayak et al, 2011;Rehman et al, 2012;Ibrahim et al, 2013;Hussain et al, 2014;Parveen et al, 2014). The plants that exhibit hepatoprotective effects, have the phytoconstituents such as phenyl compounds, coumarins, essential oils, monoterpenoids, diterpenoids, triterpenoids, steroids, alkaloids and other nitrogenous compounds (Sharma et al, 2011;Valan et al, 2010) that reveal hepatoprotective effects by preventing the liver from the damaging effects of drug-induced intoxication .…”

Section: Discussionmentioning

confidence: 99%

Hepatoprotective effects of methanolic extract of <i>Alcea rosea</i> against acetaminophen-induced hepatotoxicity in mice

Hussain

Akash

Tahir

et al. 2014

Bangladesh J Pharmacol

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“…Therefore, to design a liver protective compound is of immense importance to safeguard it from damages. The medicinal plant research constantly revealed that hepato-protective effects associated with plant-based compounds via the antioxidant properties [7][8].…”

Section: Introductionmentioning

confidence: 99%

Validation of Hepatoprotective and Antioxidant Activity of Folk Lore Knowledge of Purified Anthocyanin From Cell Suspension Culture of Clerodendron Infortunatum L.: A Search

Murukan

Saied

Aswathy

et al. 2017

Int J Pharm Pharm Sci

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Objective: To validate the hepatoprotective and antioxidant activity of purified anthocyanin extracted from the cell suspension culture of Clerodendron infortunatum Linn. Methods:A protocol has been developed for the induction of callus proliferation from leaf and nodal explants of C. infortunatum. The explants were inoculated on murashige and skoog (MS) medium supplemented with diverse combinations of 2, 4-dichlorophenoxyacetic acid (2,4-D) and benzylaminopurine (BAP) for triggering callus formation. Subsequently, the green compact callus has been sub-cultured in the medium fortified with 2,4-dichlorophenoxyacetic acid (2,4-D)and Kinetin for anthocyanin synthesis. Cell suspension culture was also established and the elicitor, salicylic acid was used for triggering anthocyanin synthesis. Three different chromatographic columns (solid phase extraction by Sepharose C18 column, Oasis-MCX and Amberlite XAD 7+Sephadex LH 120 sorbents) were employed to purify the in vitro synthesized anthocyanin from cell suspension cultures. For purity evaluation, high-performance liquid chromatography (HPLC) and molar absorptivity assay was used. Further, hepatoprotective and antioxidant activity was evaluated comparing with silymarine, as standard in rats. In vitro antioxidant scavenging activity was analysed by 2 2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC) assay.Results: After 1 mo, the leaf explants yielded remarkable green compactcallus on murashige and skoog (MS) medium containing 2.0 mg/l benzylaminopurine (BAP) and 0.5 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D). Salicylic acid enhanced anthocyanin synthesis. The mean purity values obtained by high-performance liquid chromatography (HPLC) were 90.9%±1.9 and 80.60%±2.3 for Oasis MCX, Amberlite XAD-7+Sephadex LH-20 column respectively. However, the purity calculated by molar absorptivity was found to be less. The highest purity achieved using molar absorptivity analysis was with MCX cartidges i.e., 85.9±3.8%. High-performance liquid chromatography (HPLC) yielded 12 anthocyanin fractions. Remarkable antioxidant scavenging activity was noticed as revealed by 2 2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC) assay. The hepatoprotective activity (25, 50, 100 mg/100g b. w) was compared with silymarine (25 mg/kg b. w) against carbon tetrachloride (CCl4) induced toxicity. Anthocyanin extract improved the aspartate aminotransferase (AST), alanine aminotransferase (ALT) and recovered the activity of kidney function by decreasing the urea and creatinine content. In addition, the administration of anthocyanin significantly inhibited the oxidative stress via its scavenging of the reactive oxygen species formed by carbon tetrachloride (CCl4) stress. Further, a decrease in the malondialdehyde (MDA), hydrogen peroxide (H2O2), nitric oxide (NO) accumulation and an increase of glutathione (GSH) content were noticed. Similarly, improved...

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Hepatoprotective activity ofGlycosmis pentaphyllaagainst paracetamol-induced hepatotoxicity in Swiss albino mice

Cited by 49 publications

References 12 publications

Hepatoprotective effects of Malva sylvestris L. against paracetamol-induced hepatotoxicity

Hepatoprotective effects of Malva sylvestris L. against paracetamol-induced hepatotoxicity

Hepatoprotective effects of methanolic extract of <i>Alcea rosea</i> against acetaminophen-induced hepatotoxicity in mice

Validation of Hepatoprotective and Antioxidant Activity of Folk Lore Knowledge of Purified Anthocyanin From Cell Suspension Culture of Clerodendron Infortunatum L.: A Search

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