Hepatoprotective and antidiabetic activities of Fraxinus angustifolia Vahl extracts in animal models: characterization by high performance liquid chromatography analysis

Medjahed, Zineb; Atmani-Kilani, Dina; Fauconnier, Marie‐Laure; Richard, Gaëtan; Atmani, Djebbar

doi:10.3906/sag-1502-13

Cited by 15 publications

(9 citation statements)

References 44 publications

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“…Treatment of mice with both extracts had diminished the lipid peroxidation and increase in biochemical markers of serum in streptozotocin and paracetamol induced damages in mice. The results suggest that hepato-protective and antidiabetic proficiencies might have been established by the presence of antioxidant constituents in the plant [29]. In vitro antioxidant activities of the various aqueous extracts from the bark of F. floribunda have also been reported [30].…”

Section: Introductionmentioning

confidence: 99%

Protective effects of Fraxinus xanthoxyloides (Wall.) leaves against CCl4 induced hepatic toxicity in rat

Khan

Sajid

2016

BMC Complement Altern Med

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BackgroundLeaves and root bark of Fraxinus xanthoxyloides Wall. (Oleaceae) are used locally for the treatment of jaundice, malaria and pneumonia. Decoction of stem, twigs and bark is used in pain, internal injuries, rheumatism and in bone fracture. In this investigation we have evaluated the methanol extract of leaves for its hepatoprotective potential against CCl4 induced hepatic injuries in rat.MethodsPowder of F. xanthoxyloides leaves was extracted with methanol (FXM) and subjected for the determination of polyphenolics through HPLC-DAD analysis. Sprague–Dawley (Rattus novergicus) male rats were divided into eight groups (six rats in each). Group I: non-treated control; Group II: vehicle treated (DMSO plus olive oil) while Group III- VI treated with 1 ml/kg body weight (bw) of CCl4 (30 % in olive oil) for 30 days (15 dosages) to induce the hepatic injuries. Group IV: treated with silymarin (100 mg/kg bw); Group V and VI with FXM (200, 400 mg/kg bw) on alternate days with CCl4 treatment. Group VII and VIII was administered with FXM (200, 400 mg/kg bw) alone (15 dosages). After 30 days the serum was evaluated for liver function enzymes and biochemical markers, liver samples for antioxidant enzymes, biochemical markers, comet assay and for histopathology.ResultsHPLC-DAD analysis of FXM revealed the existence of rutin and caffeic acid. In CCl4 treated rats the level of alanine transaminase (ALT), aspartate transaminase (AST), total bilirubin was significantly increased while the albumin concentration in serum was decreased as compared to control group. The level of hepatic antioxidant enzymes, catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), glutathione-S-transferase (GST) and glutathione reductase (GSR) was significantly decreased against the control group. Further, significant decrease in GSH while increase in lipid peroxides (TBARS), H2O2, DNA damages and comet length was induced with CCl4 in hepatic tissues of rat. In contrast, co-administration of FXM and silymarin restored the biochemical and histopathological status of the liver.ConclusionResults of present investigation revealed that F. xanthoxyloides leaves possibly protect the liver against CCl4 induced injuries like silymarin by its antioxidant constituents.

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Section: Introductionmentioning

confidence: 99%

Protective effects of Fraxinus xanthoxyloides (Wall.) leaves against CCl4 induced hepatic toxicity in rat

Khan

Sajid

2016

BMC Complement Altern Med

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“…29,30 Currently, chromatography analysis, as a more conventional and effective identication method of traditional medicines, commonly includes paper chromatography, thin-layer chromatography, gas chromatography (GC), and high-performance liquid chromatography (HPLC) in actual identication. [31][32][33] NMR and MS play crucial roles in the phases of component identication from novel chemical structures to Q-marker discovery in a high-throughput environment. 34 MS, as an efficient technique for measuring the peak and relative intensities, exact mass, molecular formula, as well as relative error, has been attempted to manifest the chemical structure.…”

Section: Modern Technology For Identifying Chemical Componentsmentioning

confidence: 99%

“…Gastrodia elata tuber (GET), as a famous herbal medicine, has been widely used in Asia for a long time. The study adopts a rational strategy of ngerprintefficacy relationship based on HPLC coupled with QTOF-MS method and model of b-amyloid peptide (Ab [25][26][27][28][29][30][31][32][33][34][35] )-induced PC12 cell death in vitro for exploring neuroprotective effects of the GET extracts. The result shows that 5-hydroxymethyl-2furaldehyde (5-HMF), parishin B (PB), and parishin C (PC) were identied and regarded as quality markers of GET by interpreting the ngerprint-efficacy relationship of chemical ngerprints and neuroprotective effects using subsequent orthogonal projection to the latent structure-discriminant analysis (OPLS-DA) mode.…”

Section: Based On Biosynthetic Pathway and Specicity Of Compositionmentioning

confidence: 99%

Recent advances and effective strategies in the discovery and applications of natural products

et al. 2018

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Natural products are the most representative form of conventional therapy as compared to any other traditional or alternative medicine systems. They have numerous active components, either primary or secondary metabolites, which are associated with the diverse, intricate, and distinct characteristics of natural products and result in various pharmacological effects in clinic. However, some problems are associated with research on herb quality, which is the core of the drug industry, and restrict the development of this field to a certain extent. Quality-markers (Q-markers), a novel concept for quality assessment, open up a new avenue for promotion of healthy development of traditional medicine industry and improvement of the quality standard system to enhance traditional medicine or product quality standards. In this study, we first summarized the main factors affecting the quality of traditional medicines and natural products and importance for safety and then presented the concept of background and relevant factors of Q-markers. Moreover, the modern science technology and related methods used to identify the chemical composition have been discussed. Especially, based on the systematic analysis and discussion of the basic properties and clinical features of natural products, we have discussed new trends and effective strategies for identifying relevant Q-markers from herbs and probed the future research directions and challenges.

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“…In vitro assessment of hydroxyframoside B isolated from ethanolic extract of stem bark of Fraxinus rhynchophylla provided an approach to decrease obesity via downregulating the pancreatic lipase activity thus restricting the absorption of lipid by pancreas [ 36 ]. Treatment of streptozotocin- (STZ-) induced diabetic rats by Fraxinus angustifolia leaf extract (25 and 50 mg/kg) resulting in considerable hypoglycemic effects with significant reduction in malondialdehyde levels in short interval of time providing a way for the treatment of diabetes [ 106 ]. Oral administration of hydroethanolic extracts from Fraxinus ornus at dose concentrations of 10 or 50 mg/kg body weight in nicotinamide-streptozotocin-induced diabetic mice showed potent antihyperglycemic activity.…”

Section: Other Biological Activitiesmentioning

confidence: 99%

Fraxinus: A Plant with Versatile Pharmacological and Biological Activities

Sarfraz

Rasul

Jabeen

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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Fraxinus, a member of the Oleaceae family, commonly known as ash tree is found in northeast Asia, north America, east and western France, China, northern areas of Pakistan, India, and Afghanistan. Chemical constituents of Fraxinus plant include various secoiridoids, phenylethanoids, flavonoids, coumarins, and lignans; therefore, it is considered as a plant with versatile biological and pharmacological activities. Its tremendous range of pharmacotherapeutic properties has been well documented including anticancer, anti-inflammatory, antioxidant, antimicrobial, and neuroprotective. In addition, its bioactive phytochemicals and secondary metabolites can be effectively used in cosmetic industry and as a competent antiaging agent. Fraxinus presents pharmacological effectiveness by targeting the novel targets in several pathological conditions, which provide a spacious therapeutic time window. Our aim is to update the scientific research community with recent endeavors with specifically highlighting the mechanism of action in different diseases. This potentially efficacious pharmacological drug candidate should be used for new drug discovery in future. This review suggests that this plant has extremely important medicinal utilization but further supporting studies and scientific experimentations are mandatory to determine its specific intracellular targets and site of action to completely figure out its pharmacological applications.

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Hepatoprotective and antidiabetic activities of Fraxinus angustifolia Vahl extracts in animal models: characterization by high performance liquid chromatography analysis

Cited by 15 publications

References 44 publications

Protective effects of Fraxinus xanthoxyloides (Wall.) leaves against CCl4 induced hepatic toxicity in rat

Protective effects of Fraxinus xanthoxyloides (Wall.) leaves against CCl4 induced hepatic toxicity in rat

Recent advances and effective strategies in the discovery and applications of natural products

Fraxinus: A Plant with Versatile Pharmacological and Biological Activities

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