Hepatoprotective and antioxidant activity of Cassytha filiformis against CCl4 induced hepatic damage in rats

Raj, Bincy; Singh, S. D.; Samual, Vimal John; John, Soosamma; Siddiqua, Ayesha

doi:10.1016/j.jopr.2013.01.011

Cited by 12 publications

(7 citation statements)

References 12 publications

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“…It is principal cause of the development of hepatic disorders (Galicia‐Moreno & Gutiérrez‐Reyes, ). There are different types of liver disorders such as cirrhosis, jaundice, tumors, degenerative lesions, and liver cell necrosis (Raj, Singh, Samual, John, & Siddiqua, ). Liver injury can provoked by various factors and hepatotoxins, such as carbon‐tetrachloride (CCl 4 ), ethanol, and acetaminophen, which are metabolized by cytochrome P450 2E1 (Sun et al, ).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and hepatoprotective effects ofAsparagus albusleaves in carbon tetrachloride-induced liver injury rats

Serairi-Beji

Wannes

Hamdi³

et al. 2017

J Food Biochem

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The antioxidant and hepatoprotective activities of hot aqueous extract from Asparagus albus (A. albus) leaf against CCl 4 -induced liver damage in rats were investigated. The total phenolic, flavonoid, and condensed tannin contents of hot aqueous extract from A. albus leaf were determined. The antioxidant activity of hot aqueous extract from A. albus leaf was evaluated using the antioxidant capacity, DPPH free radical-scavenging ability and reducing power assays. Different polyphenolic compounds, namely gallic acid, vanillic acid, 3,4 dimethoxybenzoic acid, catechin, rutin, and quercetin were identified. Oral administration of hot aqueous extract from A. albus leaf to male Wistar, intoxicated with CCl 4 , demonstrated a significant protective effect by lowering the levels of hepatic marker enzymes (aspartate and lactate transaminases) and by improving the histological architecture of the rat liver. The hot aqueous extract from A. albus leaf attenuated oxidative stress by restoring the activities of superoxide dismutase, catalase, and glutathione peroxidase.This study demonstrated that the hot aqueous extract from A. albus leaf protects rat liver from CCl 4 -induced injury and suggests a potential therapeutic use for A. albus plant. Practical applicationsThe hepatoprotective effects of plant extracts are mainly related to their richness in phenolic compounds. Phenolic compounds are secondary metabolites widely found in plants, mostly represented by phenolic acids and flavonoids. The growing interest in these bioactive antioxidant components is principally due to their antioxidant potential and the association between their consumption and the prevention of some diseases. In this study, a remarkable hepatoprotective effect of the hot aqueous leaf extract obtained from Asparagus albus was detected against CCl 4 -induced liver injury in rat and its mechanism was related, at least in part, to its free radical scavenging and antioxidant activity on account of the presence of polyphenols.

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Section: Introductionmentioning

confidence: 99%

Antioxidant and hepatoprotective effects ofAsparagus albusleaves in carbon tetrachloride-induced liver injury rats

Serairi-Beji

Wannes

Hamdi³

et al. 2017

J Food Biochem

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“…Liver is one of the important organ of our body and plays a vital function in the maintenance, performance and regulating homeostasis of our body [45] . Liver disorders have become one of the serious health problems and a major cause of morbidity and mortality all over the world.…”

Section: Introductionmentioning

confidence: 99%

Hepatoprotective potential of antioxidant potent fraction from Urtica dioica Linn. (whole plant) in CCl 4 challenged rats

2015

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The aim of the present study was to isolate hepatoprotective component from Urtica dioica Linn. (whole plant) against CCl4-induced hepatotoxicity in-vitro (HepG2 cells) and in-vivo (rats) model. Antioxidant activity of hydro alcoholic extract and its fractions petroleum ether fraction (PEF), ethyl acetate fraction (EAF), n-butanol fraction (NBF) and aqueous fraction (AF) were determined by DPPH and NO radicals scavenging assay. Fractions were subjected to in-vitro HepG2 cell line study. Further, the most potent fraction (EAF) was subjected to in-vivo hepatoprotective potential against CCl4 challenged rats. The in-vivo hepatoprotective active fraction was chromatographed on silica column to isolate the bioactive constituent(s). Structure elucidation was done by using various spectrophotometric techniques like UV, IR, 1H NMR, 13C NMR and MS spectroscopy. Ethyl acetate fraction (EAF) of hydro-alcoholic extract of U. dioica possessed the potent antioxidant activity viz. DPPH (IC50 78.99 ± 0.17 μg/ml) and NO (IC50101.39 ± 0.30 μg/ml). The in-vitro HepG2 cell line study showed that the EAF prevented the cell damage. The EAF significantly attenuated the increased liver enzymes activities in serum and oxidative parameters in tissue of CCl4-induced rats, suggesting hepatoprotective and anti-oxidant action respectively. Column chromatography of most potent antioxidant fraction (EAF) lead to the isolation of 4-hydroxy-3-methoxy cinnamic acid (ferulic acid) which is responsible for its hepatoprotective potential. Hence, the present study suggests that EAF of hydro-alcoholic extract has significant antioxidant and hepatoprotective potential on CCl4 induced hepatotoxicity in-vitro and in-vivo.

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“…After the last BJM suspension was given, mice were left alone for 24 h and then induced with CCL 4 diluted in coconut oil (1:1) at a dosage of 1 mL/kg mouse weight [11][12][13]. After the mice were induced, they were fed ad libitum for 24 h. After 48 h, mice were anesthetized with ether, and blood from the orbital sinus was extracted and measured for its plasma ALT and AST levels.…”

Section: Preliminary Testmentioning

confidence: 99%

Hepatoprotective Effect of Bellamya Javanica: Aspartate Transaminase, Alanine Aminotransferase, and Alkaline Phosphatase Activity, and Liver Histopathology in Mice Induced With Carbon Tetrachloride

et al. 2018

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Objective: The aim of this study was to evaluate the hepatoprotective effects of Bellamya javanica meat (BJM) in mice by quantifying alanine aminotransferase (ALT), aspartate transaminase (AST), and alkaline phosphatase (ALP) activity and observing liver histology. Methods:A total of 36 white male Sprague-Dawley mice were divided into six groups, namely a normal control group, a negative control group (0.5% carboxymethyl cellulose), a positive control group (silymarin with a dosage of 9.45 mg/200 g mouse weight), dosage 1 group (BJM powder at 1 g/kg mouse weight), dosage 2 group (BJM powder at 2 g/kg mouse weight), and dosage 3 group (BJM powder at 4 g/kg mouse weight). Mice were treated for 14 days. On the 15 th day, all groups (except the normal control group) were induced with carbon tetrachloride and fed ad libitum. After 24 h of induction, ALT, AST, and ALP in serum were quantified, and livers were dissected for histopathological examination. Results:The results showed that the consumption of BJM at doses of 2 and 4 g/kg mouse weight cast a hepatoprotective effect compared with the negative control group. In addition, there were significant differences in the hepatoprotective effect between the various doses of BJM. The dosage with the highest potential hepatoprotective effect was 4 g/kg mouse weight (p<0.05). Conclusion:B. javanica has potential hepatoprotective effects, with the strongest protection occurring at a 4 g/kg mouse weight dosage. Hepatoprotection was observed in the form of decreased AST, ALT, and ALP activity and relevant changes in liver histopathology.

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Hepatoprotective and antioxidant activity of Cassytha filiformis against CCl4 induced hepatic damage in rats

Cited by 12 publications

References 12 publications

Antioxidant and hepatoprotective effects ofAsparagus albusleaves in carbon tetrachloride-induced liver injury rats

Antioxidant and hepatoprotective effects ofAsparagus albusleaves in carbon tetrachloride-induced liver injury rats

Hepatoprotective potential of antioxidant potent fraction from Urtica dioica Linn. (whole plant) in CCl 4 challenged rats

Hepatoprotective Effect of Bellamya Javanica: Aspartate Transaminase, Alanine Aminotransferase, and Alkaline Phosphatase Activity, and Liver Histopathology in Mice Induced With Carbon Tetrachloride

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