Hepatoprotective and hypoglycemic effects of a tannin rich extract from Ximenia americana var. caffra root

Sobeh, Mansour; Mahmoud, Mona F.; Abdelfattah, Mohamed A.O.; El-Beshbishy, Hesham A.; El-Shazly, Assem M.; Wink, Michael

doi:10.1016/j.phymed.2017.07.003

Cited by 70 publications

(50 citation statements)

References 28 publications

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“…4 Several studies have demonstrated its pharmacological activity, e.g.,antiviral, 5 antimicrobial, 6 antitumor, 7 antioxidant 8 and antipyretic activities, 9 with hepatoprotective and hypoglycemic properties. 10 With the purpose of identifying plants ant natural products with pharmacological activities, our research group performed previous ethnopharmacological studies with many plants with antinociceptive and antiinflammatory activities. [11][12][13] Thus, the major aim of the current study was to evaluate the ethanol extract, fractions and one pure compound, an epicatechin derivative obtained from the stem bark of X. americana, for their antinociceptive and antiinflammatory activities.…”

Section: Introductionmentioning

confidence: 99%

Antinociceptive and Anti-inflammatory Activities of the Ethanolic Extract, of Fractions and of Epicatechin Isolated from the Stem Bark of Ximenia americana L. (Oleacaceae)

Uchôa¹,

Dias²,

Melo³

et al. 2018

Rev. Virtual Quim.

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Resumo:As ações antinociceptivas e anti-inflamatórias do extrato etanólico, das frações e da epicatequina (XM-Catequina) isolada a partir dos extratos etanólicos das cascas de Ximenia americana L. foram determinadas utilizando modelos in vivo como contorções induzidas pelo ácido acético, teste de formalina, teste de placa quente, peritonite induzida por zimosan e ensaio in vitro de inibição da ciclo-oxigenase. O teste de contorção revelou efeitos inibitórios maiores que 90% do extrato etanólico, das frações e da XM-Catequina (este isolado apresentou ID50 = ,0 μ ol / kg e efeito á i o = 99,60% . O teste de for ali a demonstrou um efeito antinociceptivo nas fases inicial (64.23%) e tardia (86.80%) com a XM-catequina, enquanto a fração clorofórmica mostrou menor efeito antinociceptivo na fase inicial (31.51%). A fração aquosa e o extrato etanólico causaram uma inibição significativa na fase tardia (73.68% e 82.40%, respectivamente). O tratamento de ratos com XM-catequina ou com a fração de acetato de etila não teve efeito antinociceptivo central no teste de placa quente. Os efeitos anti-inflamatórios foram determinados para a inflamação peritoneal induzida por zimosan e os dados indicaram que a XM-catequina, a fração hidrometanólica, o extrato etanólico e a fração em acetato de etila reduziram o número de células recrutadas em 46.02%, 35.06%, 41.45% e 38.61%, respectivamente. Estes resultados mostram que o extrato, as frações e a XM-catequina produzem respostas antinociceptivas e anti-inflamatórias. De acordo com os resultados obtidos nos ensaios de inibição da ciclooxigenase, os efeitos observados estão relacionados com a inibição de COX-1 e COX-2. As ações biológicas demonstradas no presente estudo apoiam o uso etnomedicinal desta planta.Palavras-chave: Plantas medicinais; anti-inflamatórios; corticosteroides; ciclooxigenase. AbstractThe antinociceptive and anti-inflammatory actions of the ethanolic extract, fractions and epicatechin (XM-Catechin) isolated from the ethanoic extracts of bark of Ximenia americana L. were determined utilizing in vivo models such as acetic acid-induced writhing, the formalin test, hot plate test, zymosan-induced peritonitis and in vitro cyclooxygenase inhibition assay. The writhing test revealed inhibitory effects higher than 90% of the ethanolic extract, fractions and XM-Catechin (this isolate presented ID50 = 32.03 µmol/kg and maximum effect = 99.60%). The formalin test demonstrated an antinociceptive effect in both early (64.23%) and late (86.80%) phases by XM-catechin, while the chloroform fraction showed a lower antinociceptive effect at the early phase (31.51%). The aqueous fraction and ethanolic extract caused significant inhibition at the late phase (73.68% and 82.40%, respectively). Treatment of mice with XM-catechin or with the ethyl acetate fraction had no central antinociceptive effect in the hot plate test. Anti-inflammatory effects were determined for zymosan-induced peritoneal inflammation and the data indicated that XM-catechin, the hydromethanol fraction, the ethanol...

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Section: Introductionmentioning

confidence: 99%

Antinociceptive and Anti-inflammatory Activities of the Ethanolic Extract, of Fractions and of Epicatechin Isolated from the Stem Bark of Ximenia americana L. (Oleacaceae)

Uchôa¹,

Dias²,

Melo³

et al. 2018

Rev. Virtual Quim.

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“…Another peak was detected at R t = 2.97 min with a [M‐H] − at m/z 169 and a fragment at m/z 125 with a [M‐H‐CO 2 ] − (due to lose of CO 2 moiety with of 44 mass units) was identified as gallic acid (2) . A peak with a pseudomolecular ion ( R t = 6.13) at m/z 183 and MS n ions at m / z 169, and 125) was tentatively identified as methylgallate (3) …”

Section: Resultsmentioning

confidence: 99%

“…[29] A peak with a pseudomolecular ion (R t = 6.13) at m/z 183 and MS n ions at m/z 169, and 125) was tentatively identified as methylgallate (3). [30] Also, a peak at (R t = 6.27) with a protonated molecule with a [M-H] À at m/z 337, producing daughter ion at m/z 191 [M-H-146] À due to the loss of 146 mass units equivalent to releasing of a coumaroyl moiety, was identified as 5-O-p-coumaroylquinic acid (4). [31] Similarly, a peak at R t = 6.74 showed a [M-H] À ion at m/z 367 and MS n ions at m/z 191 equivalent to quinic acid moiety, and another fragment at m/z 173 due to loss of H 2 O molecule, was identified as 4-O-feruloylquinic acid (5).…”

Section: Benzoic and Cinnamic Acid Derivativesmentioning

confidence: 99%

HPLC-DAD-ESI-MS/MS analysis of fruits from Firmiana simplex (L.) and evaluation of their antioxidant and antigenotoxic properties

Ghareeb

Mohamed

Saad

et al. 2018

Journal of Pharmacy and Pharmacology

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Objectives The secondary metabolites of the fruits of Firmiana simplex (L.) were analysed by LC-DAD-ESI-MS/MS; furthermore, we evaluated their antioxidant and antigenotoxic properties. Methods The antioxidant activity was investigated using the 2,2 0 -diphenyl-1-picrylhydrazyl radical (DPPH), the 2,2 0 -azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) and the ferric reducing antioxidant power (FRAP) assays. The antigenotoxic potential was determined via the comet assay. Key findings The ethyl acetate fraction (EtOAc) was analysed by LC-DAD-ESI-MS/MS: phenolic acids and flavonoids were the main polyphenols of the fruits. The EtOAc fraction yielded the highest content of polyphenols with 314.61 mg GAE/g extract, followed by 297.51, 153.75, 101.47, 97.19 for dichloromethane, butanol, methanol and water extracts, respectively. As expected, a strong correlation exists between the antioxidant activity of the investigated extracts and their total phenolic content. In the DPPH assay, the IC 50 value of the most active EtOAc fraction was 6.79 lg/ml, relative to 2.92 lg/ml of the standard ascorbic acid. ABTS and FRAP assays supported the results of DPPH assay. Moreover, using the comet assay, we could show that the phenol-rich EtOAc extract exhibits an antigenotoxic potential in human liver cancer cells (Hep-G2) treated with hydrogen peroxide (H 2 O 2 ) as a genotoxic agent. Conclusions The fruits of Firmiana simplex may be a good natural source of antioxidant and antigenotoxic agents.

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“…MDA is considered the index of lipid peroxidation [21]. In addition, GPx and SOD are antioxidant enzymes, involved in the catalytic detoxification of superoxide and hydrogen peroxide [22, 23].…”

Section: Discussionmentioning

confidence: 99%

Tetrahydrocurcumin Provides Neuroprotection in Experimental Traumatic Brain Injury and the Nrf2 Signaling Pathway as a Potential Mechanism

Guan

Chen

Lin

et al. 2017

Neuroimmunomodulation

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The protective effect of tetrahydrocurcumin (THC) after experimental traumatic brain injury (TBI) has been demonstrated, as demonstrated by the inhibition of oxidative stress, mitochondrial dysfunction, and apoptosis. However, the mechanisms underlying this effect are still not well understood. This study was to investigate the neuroprotective effects of THC, and its potential mechanisms, in a rat model of TBI. To this end, rats were divided into 4 groups: the sham group, the TBI group, the TBI + vehicle (V) group, and the TBI + THC group. THC or V was administered via intraperitoneal injection to rats in the TBI + V and TBI + THC groups 30 min after TBI. After euthanasia (24 h after TBI), neurological scores, brain water content, and neuronal cell death in the cerebral cortex were recorded. Brain samples were collected after neurological scoring for further analysis. THC treatment alleviated brain edema, attenuated TBI-induced neuronal cell apoptosis, and improved neurobehavioral function. In addition, NFE2-related factor 2 (Nrf2) expression was upregulated following TBI. These results suggest that THC improves neurological outcome after TBI, possibly by activating the Nrf2 signaling pathway.

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Hepatoprotective and hypoglycemic effects of a tannin rich extract from Ximenia americana var. caffra root

Cited by 70 publications

References 28 publications

Antinociceptive and Anti-inflammatory Activities of the Ethanolic Extract, of Fractions and of Epicatechin Isolated from the Stem Bark of Ximenia americana L. (Oleacaceae)

Antinociceptive and Anti-inflammatory Activities of the Ethanolic Extract, of Fractions and of Epicatechin Isolated from the Stem Bark of Ximenia americana L. (Oleacaceae)

HPLC-DAD-ESI-MS/MS analysis of fruits from Firmiana simplex (L.) and evaluation of their antioxidant and antigenotoxic properties

Tetrahydrocurcumin Provides Neuroprotection in Experimental Traumatic Brain Injury and the Nrf2 Signaling Pathway as a Potential Mechanism

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