Hepatoprotective Effect of Corm of Ensete ventricosum (Welw.) Cheesman Extract against Isoniazid and Rifampicin Induced Hepatotoxicity in Swiss Albino Mice

Abstract

Drug-induced liver injury (DILI) is one of the cumbersome health-related problems which render approximately 50% of liver failure and patients to receiving liver transplantation every year. Antituberculosis drugs such as isoniazid and rifampicin are potentially rendering hepatotoxicity. Ensete ventricosum (Welw.) Cheesman is an herbaceous perennial plant that contributes to the indigenous ethnomedicinal values for the society. This study aimed to investigate the hepatoprotective effect of corm of Ensete ventricosum (Welw.) Cheesman extracts against isoniazid and rifampicin induced hepatotoxicity in Swiss albino mice. The study was conducted on 30 Swiss albino mice randomly allocated into five groups. Group I, group II, group III, group IV, and group V were the groups in which mice were given distilled water, only isoniazid and rifampicin, isoniazid and rifampicin along with 200 mg/kg corm of Ensete ventricosum (Welw.) Cheesman extract, isoniazid and rifampicin along with 400 mg/kg corm of Ensete ventricosum (Welw.) Cheesman extract, and isoniazid and rifampicin along with silymarin per oral per day, respectively. On the 30th day of the experiment, mice were sacrificed after anesthetized, and blood was drawn for the liver function test, and the liver was also taken from each experimental mouse for histopathological evaluation. Data were entered into EpiData version 3.1 subsequently exported to SPSS version 25 for analysis by using one-way ANOVA. Plasma alanine aminotransferase (ALT) levels, aspartate aminotransferase (AST), alkaline phosphatase (ALP), and total bilirubin (TBIL) of group II mice were significantly ( p < 0.05 ) elevated as compared to group I. The group of mice treated with a corm of Ensete ventricosum (Welw.) Cheesman at a dose of 400 mg/kg (group IV) and silymarin100 mg/kg (group V) showed a significant ( p < 0.05 ) decrease in ALT, AST, ALP, and TBIL as compared to the group II. The liver section of group II showed a change in liver architecture; however, these deformities were not noticed in group IV mice. The result showed corm of Ensete ventricosum (Welw.) Cheesman extract has a very promising hepatoprotective potential against isoniazid and rifampicin induced liver injury.

Hepatoprotective effect of hydromethanol extract of Otostegia integrifolia benth leaves in isoniazid and rifampicin induced Swiss albino mice

“…In this study serum bilirubin levels in drug only induced mice showed a significant (P < 0.01) increase as compared with a normal control group of mice. This study result is in agreement with the data of other authors showing that RIF produced competition for the elimination of bilirubin by the liver [ 49 ] and a combination of INH and RIF resulted in a higher rate of inhibition hepatic clearance of bile, an increase in lipid peroxidation in the hepatocyte, and cytochrome P450 involved in the synergistic effect of RIF and INH [ 30 , 50 , 51 ]. Whereas O. integrifolia leaves extract induced mice challenged with INH and RIF (G IV) did not show a significant difference as compared with the normal control group.…”

Nephroprotective Effect of Asparagus africanus Lam. Root Extract against Gentamicin-Induced Nephrotoxicity in Swiss Albino Mice

“…On the 15 th day, the mice were fasted overnight and anesthetized with 100 mg/kg ketamine and 12.5 mg/kg xylazine injections. The mice were euthanized by cervical dislocation after 2–2.5 mL of blood collection through cardiac puncture [ 33 , 39 ].…”

“…The obtained serum was stored at −80°C until used for the biochemical assay. Serums were analyzed according to the standard principles and procedures outlined in the kit manufacturer's manual (SD Biosensor, Inc., Korea) [ 39 ].…”

Amelioration of Nephrotoxicity in Mice Induced by Antituberculosis Drugs Using Ensete ventricosum (Welw.) Cheesman Corm Extract

“…Additionally, other studies conducted on the nephroprotective effects of Ficus religiosa [7], Petroslinum crispums [41], and Turmeric [14] have been consistent with the present finding. is may happen due to cells' protective activities of phytochemicals that are found in EVCE [24].…”

“…Cheesman is exploited for treating kidney stones, dysuria, liver diseases, expulsion of the placenta, cough, dysentery, healing of bone fractures, diabetes, and microbial infections. It also serves as a staple and costaple food [21][22][23][24]. Several bioactive constituents and minerals in the extract of corm of the Ensete genus have cell-protective effect and enhance the antioxidant defense system (AODS) of cells by scavenging free radicals [20,[25][26][27][28].…”