Hepatoprotective Effect of Silver Nanoparticles at Two Different Particle Sizes: Comparative Study with and without Silymarin

Abstract

Silver nanoparticles have been used for numerous therapeutic purposes because of their increased biodegradability and bioavailability, yet their toxicity remains questionable as they are known to interact easily with biological systems because of their small size. This study aimed to investigate and compare the effect of silver nanoparticles’ particle size in terms of their potential hazard, as well as their potential protective effect in an LPS-induced hepatotoxicity model. Liver slices were obtained from Sprague Dawley adult male rats, and the thickness of the slices was optimized to 150 μm. Under regulated physiological circumstances, freshly cut liver slices were divided into six different groups; GP1: normal, GP2: LPS (control), GP3: LPS + AgNpL (positive control), GP4: LPS + silymarin (standard treatment), GP5: LPS + AgNpS + silymarin (treatment I), GP6: LPS + AgNpL + silymarin (treatment II). After 24 h of incubation, the plates were gently removed, and the supernatant and tissue homogenate were all collected and then subjected to the following biochemical parameters: Cox2, NO, IL-6, and TNF-α. The LPS elicited marked hepatic tissue injury manifested by elevated cytokines and proinflammatory markers. Both small silver nanoparticles and large silver nanoparticles efficiently attenuated LPS hepatotoxicity, mainly via preserving the cytokines’ level and diminishing the inflammatory pathways. In conclusion, large silver nanoparticles exhibited effective hepatoprotective capabilities over small silver nanoparticles.

Potential effects of nano-cellulose and nano-silica/polyvinyl alcohol nanocomposites in the strengthening of dyed paper manuscripts with madder: an experimental study

“…The technical information of AFM equipment are: AFM 5600LS, Agilent, Santa Clara, CA, USA. The sample preparation for AFM investigation was performed according to Elfaky et al 50 .…”

Assessment of Silver Nanoparticles Derived from Brown Algae Sargassum vulgare: Insight into Antioxidants, Anticancer, Antibacterial and Hepatoprotective Effect

“…Kunjiappan et al [50] reported that the gold nanoparticles biogenically synthesized by Azolla microphylla extract confirmed effective antioxidant and hepatoprotective effects. The large size of silver nanoparticles, compared to the small size of silver nanoparticles, displayed more hepatoprotective capabilities [51]. Cytocompatibility screening assays support a grade of cell death produced by bionanomaterial when exposed to normal cells; the results of cytotoxicity assay revealed that silver nanoparticles, even at higher concentrations, have nontoxic normal cells (human fibroblast (L929) cell lines); these results open new possibilities for their application in various biomedical fields [52].…”

Impact of aqueous leaf extract of Punica granatum and synthesized silver nanoparticles against streptozotocin induced diabetes in rats

“…Figure 3 shows the results of UV-vis analysis of AgPg aq . According to several research [22][23][24][25][26], a surface plasmon resonance (SPR) peak with a wavelength between 400 and 490 nm suggests that the particle under investigation is in the nano range (below 100 nm).…”

“…The synthesized nanoparticles must then be characterized for properties such as size, shape, and stability using a variety of techniques such as scanning electron microscopy (SEM), ultraviolet visible spectrophotometer (UV-Vis), Fourier transform infrared spectroscopy (FT-IR), dynamic light scattering analysis (DLS), and zeta potential analysis [23,24]. Many workers have reported that the silver nanoparticles synthesized using plant extracts have enhanced alleviating effect as compared to extract alone on various parameters of mammalian model in laboratory conditions [25][26][27][28].…”