Hepatoprotective Effect of β-Chitosan from Gladius of Sepioteuthis lessoniana Against Carbon Tetrachloride-Induced Oxidative Stress in Wistar Rats

Abstract

Chitosan has attracted much attention as a biomedical material, owing to its unique biological activities. In this study, hepatoprotective effect of β-chitosan obtained from the gladius of squid Sepioteuthis lessoniana was studied against carbon tetrachloride (CCl4)-induced oxidative stress and liver injury in rats. The rats that received β-chitosan along with the administration of CCl4 showed significantly decreased plasma and tissue alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities and total cholesterol, triglyceride (TG) and free fatty acid (FFA) contents, whereas the treatment with β-chitosan alone markedly increased rat hepatic and circulatory superoxide dismutase (SOD), catalase and glutathione peroxidase (GPx) and reduced glutathione (GSH) levels and decreased the malondialdehyde level. Histopathological observations recommended the marked hepatoprotective effect of β-chitosan. The CCl4-induced alterations on circulatory and hepatic antioxidant defence system were normalised by β-chitosan, and it could be concluded that the hepatoprotective effect of chitosan may be due to its antioxidant and antilipidemic property. Therefore, β-chitosan could be considered as antihepatotoxic agent.

Possible Synergistic Effect and Antioxidant Properties of Chitosan Nanoparticles and Quercetin against Carbon Tetrachloride-Induce Hepatotoxicity in Rats

“…In the same concern, Subhapradha et al [94] reported the hepatoprotective effect of β-Chitosan from Gladius of Sepioteuthis lessoniana against CCl 4 -induced oxidative stress in rats. These authors showed that, in addition to normalizing the oxidative stress markers, which is attributed to the antoxidant properties of chitosan, the normalization of plasma AST and ALT levels in β-chitosan and CCl 4 -treated rats indicates that β-chitosan may stabilize the cell membrane and may prevent a leakage of intracellular enzymes into the blood.…”

Pulsed Electromagnetic Fields Alleviates Hepatic Oxidative Stress and Lipids Accumulation in db/db mice

“…Nowadays, there are evidences suggesting that ROS plays the causal role in liver lipid droplets formation, while some others with the results that abnormal accumulation of lipid in liver induced ROS overproduced 23, 24 . Thus, ROS serves as a key driving force to exacerbates the progression from initial hepatic steatosis to the further NAFLD.…”

The use of marine-derived bioactive compounds as potential hepatoprotective agents

“…Yan et al demonstrated the protective effect of chitosan oligosaccharide and its derivatives against CCl 4 -induced liver damage by pretreatment [55] . β-Chitosan isolated from the squid Sepioteuthis lessoniana showed hepatoprotective activity against CCl 4 -induced oxidative stress in Wistar rats [56] . Along with the stabilization of various liver marker enzyme activities, histopathological observations also suggested that the hepatoprotective activity of β-chitosan is possibly due to its antioxidant and antilipidemic properties [56] .…”

“…β-Chitosan isolated from the squid Sepioteuthis lessoniana showed hepatoprotective activity against CCl 4 -induced oxidative stress in Wistar rats [56] . Along with the stabilization of various liver marker enzyme activities, histopathological observations also suggested that the hepatoprotective activity of β-chitosan is possibly due to its antioxidant and antilipidemic properties [56] . Recently, another study by Ramasamy et al showed the antioxidative and hepatoprotective roles of chitosan from Sepia kobiensis against CCl 4 -induced liver damage in rats [57] .…”