Hepatoprotective effects exerted by Poria Cocos polysaccharides against acetaminophen-induced liver injury in mice

Wu, Ka; Fan, Jie; Huang, Xing; Wu, Xinmou; Guo, Chao

doi:10.1016/j.ijbiomac.2018.03.107

Cited by 94 publications

(55 citation statements)

References 25 publications

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“…And then, the sections were developed with diaminobenzidine for immunohistochemistry stains, and DAPI for immunofluorescence stains. Subsequently, the immunohistochemical sections were photographed by using an optical microscope (Olympus, Japan) and the immunofluorescence sections were imaged through an inverted fluorescent microscope (Olympus, Japan) …”

Section: Methodsmentioning

confidence: 99%

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Network analysis, and human and animal studies disclose the anticystitis glandularis effects of vitamin C

Guo

Liang

et al. 2019

BioFactors

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Background Our present study aimed to unravel the therapeutic biotargets of vitamin C (VC) against cystitis glandularis (CG), and to elucidate the molecular mechanisms for VC treating CG. Methods Network pharmacology was used to predict therapeutic targets of VC against CG, and to identify molecular mechanisms. In addition, further human and animal studies were designed to validate the bioinformatic findings through biochemical tests, computerized tomography scans, and immunostaining assays. Results In bioinformatic analyses, pathogenic targets of CG and putative targets of VC were identified, respectively. An interaction network between biological target and functional protein was produced before screening and collecting the key therapeutic targets of VC against CG, biological processes, and signaling pathways. In addition, ingenuity pathway analysis with cloud platform indicated that anti‐CG mechanisms of VC were achieved through modulating a cluster of molecular pathways, such as tumor necrosis factor (TNF) pathway. Meanwhile, 18 core targets of VC against CG were identified, and the most important TNF, interleukin‐6 (IL6), and Jun biotargets were obtained, respectively. In further validation in human study, cellular TNF‐α, IL6, and c‐Jun expressions in patient's CG samples were elevated significantly, accompanied with detectable urinary tract infection. Beneficially, VC‐dosed CG mice resulted in downregulated expressions of endogenous TNF‐α, IL6, and c‐Jun in blood and bladder samples. Conclusion Collectively, these bioinformatic findings and experimentative data uncover the therapeutic targets and biological mechanisms of VC for treating CG, in which the key biomarkers of TNF‐α, IL6, and c‐Jun may be the potential molecules for treating CG in clinical application.

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Section: Methodsmentioning

confidence: 99%

“…Subsequently, the immunohistochemical sections were photographed by using an optical microscope (Olympus, Japan) and the immunofluorescence sections were imaged through an inverted fluorescent microscope (Olympus, Japan). 18,19…”

Section: Immunostaining Protocolsmentioning

confidence: 99%

Network analysis, and human and animal studies disclose the anticystitis glandularis effects of vitamin C

Guo

Liang

et al. 2019

BioFactors

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“…The sections were developed with hydrogen peroxidase enzyme‐labeled diaminobenzidine dye. Subsequently, all sections were redyed with hematoxylin in nucleoli …”

Section: Methodsmentioning

confidence: 99%

“…Subsequently, all sections were redyed with hematoxylin in nucleoli. 12,13 2.8 | Immunofluorescence staining Briefly, 5-μm dewaxed liver sections were blocked with 5% bovine serum albumin buffer for at least 1 hr and then were incubated with primary antibody of FGF21 (1:200; Abcam, UK), poly (ADP-ribose) polymerase (PARP), Caspase 3 (1:200; Cell Signalling, Danvers, USA), and Erk1/2 (Beyotime Biotechnology, Nanjing, China) overnight at 4 C. After being washed with phosphate-buffered saline (PBST) buffer for three times, the sections were further incubated with immunofluorescencespecific IgG H&L kits (Alexa Fluor 488/647, Abcam, UK) for 1 hr at 37 C, and then 4 0 ,6-diamidino-2-phenylindole (DAPI, Abcam, UK) dye was used for nuclear staining prior to being photographed and assayed. 14,15 2.9 | Real-time polymerase chain reaction test Isolation of hepatocellular RNAs was conducted by using TRI Reagent (Thermo Fisher Scientific, USA) according to the handbook's instruction.…”

Section: Immunohistochemical Stainmentioning

confidence: 99%

Potential biomarker of fibroblast growth factor 21 in valproic acid‐treated livers

Guo

Liang

et al. 2019

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Background Valproic acid (VPA) is a clinical medicine primarily prescribed to control epileptic symptoms. VPA has potential side‐effects, such as hepatotoxicity. Fibroblast growth factor 21 (FGF21) is a functional cytokine for metabolic regulation. In this article, we aimed to evaluate the possible clinical application of FGF21 in VPA‐treated livers in early undetected liver injury (EULI). Methods Methodologically, plasma samples of VPA‐treated epileptic patients were isolated for biochemical and high‐performance liquid chromatography tests. In addition, VPA‐dosed mice were subjected to determinations of serological parameters, key regulatory effectors and FGF21 expressions through biochemical analyses, enzyme‐linked immunosorbent assay, immunohistochemistry stain, immunofluorescence stain, and reverse transcription‐polymerase chain reaction (RT‐PCR) test, respectively. Results The serological data suggested that VPA‐treated epileptic patients showed visibly elevated FGF21 contents in plasma samples. However, other diagnostic parameters showed inconspicuous changes. As revealed in animal study, VPA‐dosed mice exhibited undetected morphological alterations and hormonal changes in the liver, pancreas, and kidneys. Furthermore, serological parameters and key regulatory proteins in VPA‐dosed livers and controls showed inconspicuous changes. Interestingly, endogenous FGF21 expressions in VPA‐dosed mice were increased in sera. In further experiments, the findings showed that intracellular expressions of FGF21 mRNA and protein were upregulated in VPA‐dosed livers as revealed in RT‐PCR and immunoassay. Conclusions Taken together, these preliminary data reveal that functional FGF21 cytokine may serve as a potent predictor in VPA‐related EULI.

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“…As described in immunofluorescence procedures, de-waxed pancreatic sections were blocked with 5% bovine serum albumin buffer (Beyotime Biotechnology, China) for 1 h at 37 ° C, followed by incubating with insulin, Ngn3, PDX1, F-box and WD-40 domain protein 7 (Fbxw7), CK19 (1: 50; Bioss, Beijing, China) overnight at 4 ° C. After being washed with buffer 3 times, the sections were re-incubated with goat anti-rabbit IgG H&L (Alexa Fluor 488, 594; 1: 100; Abcam, UK) for 1 h at 37 ° C. Subsequently, DAPI (Abcam, UK) was used to nuclear staining before checked and imaged steps [11,12].…”

Section: Immunofluorescence Stainingmentioning

confidence: 99%

Immunophenotypes of Ductal Epithelial Cells in Advanced Pancreatic Ductal Adenocarcinoma

Zhou

Liu

et al. 2018

Digestion

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Background/Aims: In this report, we aimed to investigate the distribution and characterization of biomarker-immunolabeled ductal epithelium in advanced pancreatic ductal adenocarcinoma (PDAC). Design: Eighteen patients with PDAC were medically diagnosed prior to being treated periodically. All clinical records were collected and assayed. The PDAC samples were subjected to routine biochemical tests and immuno-stains of immunohistochemistry and immunofluorescence. Results: As results, immunohistochemical findings indicated pancreatic ductal cells in PDAC showed plenty of Ki-67, P53, RP, SAM positive cells expressed in nuclei. In addition, ductal epithelial cells exhibited numerous positive cells of CK7, 18, 19, 20 biomarkers, respectively. Interestingly, compared to non-PDAC controls, immunostaining results showed that endocrine hormones were positively expressed in pancreatic ducts of PDAC, characterized with increased insulin-, Ngn3-, PDX1-fluorescence-labeled cells, and reduced F-box and WD-40 domain protein 7 (Fbxw7)-labeled cells in ducts. Conclusion: These clinicopathologic findings preliminarily disclose that there may be a potential for insulin-producing cells in PDAC, possibly through negatively inducing Fbxw7 ubiquitination in pancreatic ducts.

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Hepatoprotective effects exerted by Poria Cocos polysaccharides against acetaminophen-induced liver injury in mice

Cited by 94 publications

References 25 publications

Network analysis, and human and animal studies disclose the anticystitis glandularis effects of vitamin C

Network analysis, and human and animal studies disclose the anticystitis glandularis effects of vitamin C

Potential biomarker of fibroblast growth factor 21 in valproic acid‐treated livers

Immunophenotypes of Ductal Epithelial Cells in Advanced Pancreatic Ductal Adenocarcinoma

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