Hepatoprotective effects of Gentiana asclepiadea L. extracts against carbon tetrachloride induced liver injury in rats

Mihailović, Vladimir; Mihailović, Mirjana; Uskoković, Aleksandra; Arambašić, Jelena; Mišić, Danijela; Stanković, Vesna; Katanić, Jelena; Mladenović, Milan; Solujić, Slavica; Matić, Sanja

doi:10.1016/j.fct.2012.10.034

Cited by 71 publications

(42 citation statements)

References 44 publications

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“…Overall, histological analysis supported the results obtained from the serum enzyme and other biochemical parameters showing less damage in the cytoarchitecture of the liver. Similar findings were observed by Mihailovic et al 15 for the hepatoprotective effects of Gentiana asclepiadea. Recently, Ravichandra et al 16 also reported that histopathological studies of the liver intoxicant group caused fatty changes, granular degeneration and inflammation, coagulative necrosis, degenerative necrosis and bile duct hyperplasia.…”

Section: Histopathological Findingssupporting

confidence: 79%

In vivo Pharmacological Effectiveness of Heat-treated Cucumber (Cucumis sativus L.) Juice against CCI4- induced Detoxification in a Rat Model

Bajpai¹,

Kim²,

Park³

et al. 2017

IJPER

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Background: Detoxification of heavy metals such as carbon tetrachloride (CCl 4 ) poisoning has become a major focus of researchers. Objectives: In this study, heat-treated cucumber juice was assessed for its pharmacological effect on protection of carbon tetrachloride (CCl 4 )-induced acute liver and kidney damages. Methods: Initially, during detoxification of CCl 4 , the rats of all groups except the control group (without treatment) were injected with a single intraperitoneal dose of 2.5 mL CCl 4 /kg with 50% concentration (1:1 ratio; v/v in olive oil). Additionally, CCl 4 -treated rats were also dosed with saline, 10 mg/kg, 100 mg/kg, and 500 mg/kg cucumber juice for 1, 3 and 5 days once in a day after 6 h from CCl 4 administration. Results: As a result, the best concentration of cucumber juice was noted to be 10 mg/kg, which showed remarkable protective effect on body weight (212-217 g), enzymatic activities of aspartate aminotransferase (AST; 53 IU/L), alanine aminotransferase (ALT; 125 IU/L), blood urea nitrogen (BUN; 18.8 mg/dL), and creatinine content (0.65 mg/dL) as compared to control (220-232 g; 477 IU/L; 987 IU/L; 16.8 mg/dL; and 0.95 mg/dL), respectively. Moreover, histology and histomorphometry analysis of liver and kidney led to significant reduction in percentage of hepatopathy and nephropathy parameters such as hepatic (46.90±2.63%) and kidney (39.86±2.01) degenerative regions, number of hepatocytes (638.00±47.25), tubules (327.60±26.67) and glomerulus (29.20±1.36). Conclusion: These findings suggest that heat-treated cucumber juice has a significant pharmacological effect on CCl 4 -induced acute liver and kidney damages in experimental rats.

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Section: Histopathological Findingssupporting

confidence: 79%

In vivo Pharmacological Effectiveness of Heat-treated Cucumber (Cucumis sativus L.) Juice against CCI4- induced Detoxification in a Rat Model

Bajpai¹,

Kim²,

Park³

et al. 2017

IJPER

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“…As one of the principal cause of CCl 4 induced liver injury is lipid peroxidation, which is induced and accelerated by free radical derivatives of CCl 4 , leading to the liver lipid peroxidation (Mihailovic et al 2013). Thus, scavenging free radicals generation is important in terms of protecting the liver from CCl 4 intoxication.…”

Section: Discussionmentioning

confidence: 99%

“…The experiments were performed based on animal ethics guidelines of Jadavpur University Animals Ethics Committee. After 7 days of acclimatization, the rats were randomly divided into nine groups (n=6) for the experiment conducted over a period of fourteen consecutive days (Mihailovic et al 2013). Group I was served as normal control (0.9 % normal saline; 1 ml/kg bw/day i.p.)…”

Section: Animals and Experimental Designmentioning

confidence: 99%

Inhibitory effects of Anthocephalus cadamba stem bark fractions intercede anti-inflammatory and carbon tetrachloride induced hepatotoxicity in rats

Dolai

Kumari

Islam³

et al. 2015

Orient Pharm Exp Med

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Anthocephalus cadamba (Roxb.) Miq. (Rubiaceae) is commonly known as BKadamba^, frequently found in moist deciduous evergreen forests. The present study was performed to evaluate the anti-inflammatory and hepatoprotective potential of A. cadamba fractions in relation to the free radical scavenging properties to corroborate and establish scientific evidence for its ethno-botanical uses. Quantification assay were determined for total phenolic and flavonoid compounds. Free radical scavenging properties were assessed by 1, 1-Diphenyl-2-picryl-hydrazyl (DPPH), nitric oxide, peroxynitrite, superoxide, hypochlorous acid scavenging methods. Protein denaturation and human red blood cell membrane stabilization methods were performed to determine anti-inflammatory activity. Furthermore, Hepatocuretive potential was evaluated using carbon tetrachloride intoxicated (CCl 4 ) rat's model. Methanol fraction of A. cadamba (MEAC) was found to be the most potent free radical scavenger in all in vitro models. The higher inhibition properties of MEAC positively correlated with total phenolic (164.75±3.83 mg GAE/g) and flavonoid compounds (51.20± 2.15 mg QE/g). The MEAC showed significant (p<0.01) antiinflammatory activity in all in-vitro methods. The A. cadamba significantly (p<0.01) decreased levels of serum glutamic oxaloacetate transaminase, serum glutamic pyruvic transaminase, alkaline phosphatase and total bilirubin in the treated groups when compare to CCl 4 control group. Treatment with A. cadamba significantly (p<0.01) increase in the levels of catalase, superoxide dismutase and reduced glutathione, accompanied with a marked reduction in the levels of malondialdehyde, as compared to CCl 4 control group. These results indicated that A. cadamba possess anti-inflammatory and hepatoprotective property which may be mediated through augmentation of antioxidant defenses.

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“…The increase in the serum ALT and AST levels by CCl 4 is attributed to the hepatic structural damage because these enzymes are normally localized to the cytoplasm and released into the circulation after the cellular damage has occurred 25 . The serum AST and ALT levels significantly decreased in the pre-and post-treatment groups of aqueous extract of P. pashia suggesting that it effectively ameliorates the hepatotoxicity induced by CCl 4 .…”

Section: Fig 3: Effects Of P Pashia On the Liver Histopathological mentioning

confidence: 99%

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2017

IJPSR

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ABSTRACT:The study was to evaluate the hepatoprotective activity of aqueous extract of Pyrus pashia Buch.-Ham. ex D. Don against carbon tetrachloride-induced hepatotoxicity in mice. Hepatotoxicity was induced by CCl 4 30% in olive oil (1ml/kg i.p.). Mice were treated with aqueous extract of P. pashia at doses of 250 and 500 mg/kg b.wt. orally for 14 days. There were two group pre-treatment (once daily for 14 days before CCl 4 intoxication) and post-treatment (2, 6, 24 and 48 hr after CCl 4 intoxication) groups. The observed effects were compared with a known hepatoprotective agent, silymarin. Pre-treatment and post-treatment groups of aqueous extract of P. pashia significantly reduced elevated serum levels of serum transaminases, alkaline phosphatase, and bilirubin and increased the level of total protein as compared to CCl 4 treated group. The histopathological study also confirms the hepatoprotection. Preliminary qualitative phytochemical analysis of the plant revealed the presence of carbohydrates, phenolic compounds, tannins, and saponins. The data are expressed as ± S.E.M. one-way analysis of variance (ANOVA) followed by multiple comparisons with the Tukey post hoc test to compare different parameters between the groups.

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Hepatoprotective effects of Gentiana asclepiadea L. extracts against carbon tetrachloride induced liver injury in rats

Cited by 71 publications

References 44 publications

In vivo Pharmacological Effectiveness of Heat-treated Cucumber (Cucumis sativus L.) Juice against CCI4- induced Detoxification in a Rat Model

In vivo Pharmacological Effectiveness of Heat-treated Cucumber (Cucumis sativus L.) Juice against CCI4- induced Detoxification in a Rat Model

Inhibitory effects of Anthocephalus cadamba stem bark fractions intercede anti-inflammatory and carbon tetrachloride induced hepatotoxicity in rats

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