Inhibitory effects of Anthocephalus cadamba stem bark fractions intercede anti-inflammatory and carbon tetrachloride induced hepatotoxicity in rats

Dolai, Narayan; Kumari, Uma; Islam, Aminul; Haldar, Pallab Kanti

doi:10.1007/s13596-015-0182-x

Cited by 4 publications

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References 21 publications

(38 reference statements)

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“…[ 3 ] This medicinal plant has been used for the treatment of tumor, fever, hematological diseases, uterine complaints, skin diseases, hypoglycemic agent, reduces pain, and inflammation. [ 4 5 ] Earlier reports from bioactivity determination provided evidence for its cytotoxic effect on human cancer cell lines,[ 6 ] free radical scavenging and anti-inflammatory,[ 7 ] antidiabetic,[ 8 ] antioxidant, antimicrobial, and wound healing activities. [ 3 ] The stem bark has a wide range of chemical constituents, namely, cadamine, isocadamine, cadambine, 3α-dihydrocadambine, isodihydrocadambine, and chlorogenic acid.…”

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confidence: 99%

Methanolic extract of Anthocephalus cadamba induces apoptosis in Ehrlich ascites carcinoma cells in experimental mice

Dolai

Islam²,

Haldar

2016

Indian J Pharmacol

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Objective:Anthocephalus cadamba (Roxb.) Miq. (Family: Rubiaceae), a folk medicine commonly known as “Kadam” in Bengali, has been used for the treatment of tumor. The methanolic extract of A. cadamba (MEAC) showing antitumor activity on Ehrlich ascites carcinoma (EAC) cells treated mice was already reported. This study was designed to study the apoptosis-inducing property of MEAC and its mechanism in EAC cells in mice.Materials and Methods:Apoptogenic morphology was determined by fluorescent DNA-binding double staining method using dyes acridine orange (AO)/ethidium bromide (EB). Comet assay was estimated to check the DNA damage. Flow cytometry (fluorescence-activated cell sorting [FACS]) was used to detect the apoptotic rate quantitatively by double labeling techniques using annexin V FITC/propidium iodide staining. Apoptotic protein expression was done using Western blotting assay method.Statistical Analysis:Results are expressed as mean ± standard deviation. Statistical analysis was performed using ANOVA followed by Dunnett's post hoc test of GraphPad Prism software. *P < 0.05, **P < 0.01 and ***P < 0.001 were considered statistically significant.Results:Apoptosis-inducing effect of MEAC on EAC cells was confirmed from AO/EB staining and FACS analysis. MEAC treatment showed dose-dependent induction of DNA damage. Apoptosis was induced by increasing the expression of multiple downstream factors such as pro-apoptotic protein p53 and p21 in EAC. Bax was up-regulated and anti-apoptotic protein Bcl-2 was down-regulated resulting in decrease of the Bcl-2/Bax ratio by MEAC treatment.Conclusion:Experimental results revealed that MEAC induces apoptosis by modulating the expression of some pro-apoptotic and anti-apoptotic proteins in EAC and thus exerts its anti-tumor activity.

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confidence: 99%

Methanolic extract of Anthocephalus cadamba induces apoptosis in Ehrlich ascites carcinoma cells in experimental mice

Dolai

Islam²,

Haldar

2016

Indian J Pharmacol

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“…Its stem bark extract exhibited potent antiinflammatory properties in vivo experiments by suppressing histamine induced paw edema and cotton pellet induced granuloma whereas in vitro experiments, the bark fractions have shown protein anti-denaturation and human RBC membrane stabilization activities [104][105][106][107]. Again, extracts from leaf, fruit and flowering top of A. cadamba exhibited significant human RBC membrane stabilization action [108][109][110][111].…”

Section: Anthocephalus Cadamba (Roxb) Miqmentioning

confidence: 99%

Management of inflammatory disorders: A brief review of selected ethnomedicinal plants

Akhtar,

Khan,

Zahan

2023

JPP

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Medicinal plants have immense significance to treat various diseases and have great potential to discover and develop new therapies. This paper aims to summarize the in vitro and in vivo investigations conducted to evaluate the anti-inflammatory activities of selected medicinal plants of Bangladesh. The anti-inflammatory active molecules reported from those selected plants have also been highlighted. The species included in this review are Abelmoschus esculentus (L.) Moench, Asparagus racemosus Willd., Blumea lacera (Burm.f.) DC., Butea monosperma (Lam.) Taub., Cheilocostus speciosus (J.Koenig) C.D.Specht., Mussaenda roxburghii Hook. f., Anthocephalus cadamba (Roxb.) Miq., Phyllanthus reticulatus Poir., Sesbania grandiflora (L.) Pers. and Spondias pinnata (L. f.) Kurz. The toxic effect of these plants has also been included briefly. We hope that this overview will shed some light on the function of these plants and their molecules in the treatment of various inflammatory diseases and will attract the attention of investigators interested in designing novel therapeutic approaches.

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Chemical Characterization, In-silico Evaluation, and Molecular Docking Analysis of Antiproliferative Compounds Isolated from the Bark of Anthocephalus cadamba Miq.

Ansari

Ahmad

Fatima

et al. 2022

ACAMC

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Aim: The aim of the present study was to isolate and characterise chemical compounds from Anthocephalus cadamba Miq. bark and evaluate their anticancer activity by in silico, molecular docking and in vitro studies. Background: Anthocephalus cadamba is a traditionally used Indian medicinal plant. The anticancer and phytochemical investigations of this plant bark remain unexplored except only with few studies. Objectives: The objective of the study was to evaluate antiproliferative activity of extract and fractions against breast cancer and prostate cancer cell lines. Isolation and characterisatoion of active compounds from bio-active guided fractions. Evaluation of anticancer activity of isolated compounds against breast and prostate cancer cell lines. In addition to in silico and molecular docking interactions of isolated compounds with VEGFR2 and PDGFRα target proteins. Methods: The compounds were isolated and purified with the help of repeated column chromatography, and spectral techniques 1D, 2D NMR, GC-MS/MS were used to identify and elucidate the structure of compounds. Moreover, prediction of activity spectra for substances, physiochemical properties, bioactivity radar prediction, bioactivity score, natural-product likeness, ADME, and toxicity parameters of isolated compounds (AC-1 to AC-4) was performed through various in-silico databases and servers. To evaluate the docking interaction profile and binding energies of compounds, three docking tools were utilized AutoDock, AutoDock Vina, and iGEMDOCK, against two targets VEGFR2 and PDGFRα. MD simulation was performed through ligand and receptor molecular dynamic server (LARMD). Results: The study has shown that the A. cadamba bark chloroform fraction demonstrated a significant inhibitory effect against MDA-MB-231, MCF-7 and PC-3 cells in a dose-time dependent manner. The bioassay guided isolation afforded four molecules AC-1 to AC-4 from chloroform fraction. Moreover, the GC-MS/MS profiling identified fourteen new molecules which were not reported earlier from A. cadamba. The in-silico study showed that the isolated compounds (AC-1 to AC-4) obey Lipinski’s rule and had good oral bioavailability. While compound AC-4 had positive bioactivity scores except for kinase inhibitor activity. The ADMET profiling revealed that AC-4 was non-toxic and easily absorbed in the human intestine, and transportable in the blood-brain barrier when compared with AC-1, AC-2, AC-3 and standard drug doxorubicin. Molecular docking and MD simulation assessment also signified AC-4 anticancer activity with dual inhibitory action against the target proteins VEGFR2 and PDGFRα amongst the studied compounds. The in vitro cell viability assay of isolated compounds demonstrated that AC-1 showed IC50 (μg/mL) value of 34.96 ±3.91, 47.76±3.80 69.1±4.96, AC-2; 68.26±4.22, 54.03±5.14, >100, AC-3; 35.34±4.14, 51.5±51.5, 70.8±5.25 and AC-4; 44.2±3.57, 24.2±2.67, 51.2±2.54 for MDA-MB-231, MCF-7 and PC-3 cancer cell lines, respectively and compared with standard drug doxorubicin. Moreover, fluorescence microscopy confirmed the apoptogenic property of compounds. We also found that AC-4 exhibited significant intracellular ROS production in breast cancer cells which induces apoptosis and eventually cell death. Conclusion: In conclusion, A. cadamba afforded four pure molecules AC-1 to AC-4 with identification of fourteen new compounds. The entire in-silico studies concluded that the AC-4 compound had better oral bioavailability, bioactivity score, and ADMET profile amongst studied molecules. Molecular docking analysis and MD simulation also supported AC-4 dual inhibitory action against both VEGFR2 and PDGFRα receptors. Moreover, the isolated molecules AC-1, AC-2, AC-3 and AC-4 were found to be active against MDA-MB-231, MCF-7 and PC-3 cancer cells. The molecule AC-4 induces ROS mediated apoptosis in breast cancer cells. The anticancer inhibitory potentiality of AC-4 is directed to its molecular stereochemistry which specifically binds to the target proteins of breast cancer cells with no toxicological effect. Therefore, AC-4 is suggested to be an effective aspirant for the novel drug design and discovery.

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Inhibitory effects of Anthocephalus cadamba stem bark fractions intercede anti-inflammatory and carbon tetrachloride induced hepatotoxicity in rats

Cited by 4 publications

References 21 publications

Methanolic extract of Anthocephalus cadamba induces apoptosis in Ehrlich ascites carcinoma cells in experimental mice

Methanolic extract of Anthocephalus cadamba induces apoptosis in Ehrlich ascites carcinoma cells in experimental mice

Management of inflammatory disorders: A brief review of selected ethnomedicinal plants

Chemical Characterization, In-silico Evaluation, and Molecular Docking Analysis of Antiproliferative Compounds Isolated from the Bark of Anthocephalus cadamba Miq.

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