Hepatoprotective effects of walnut oil and Caralluma tuberculata against paracetamol in experimentally induced liver toxicity in mice

Abstract

Walnut Oil and Caralluma are edible and form part of the traditional medicine system in many countries. These are frequently used in traditional medicine as remedies to relieve a wide range of illnesses and health problems. Walnut Oil and Caralluma species have demonstrated anti-inflammatory, anti-nociceptive, antidiabetics, hepatoprotective, gastric mucosa protecting, antimalarial, antioxidant, anti-trypanosomal, appetite suppressant and cytotoxic activities. The current study was planned to study the impacts of 21 days’ oral administration of walnut oil and methanolic extract of Caralluma tuberculata on the levels of some liver-associated parameters and hematological parameters in paracetamol intoxicated mice. It was observed that paracetamol intoxication resulted in a considerable rise in serum ALT, cholesterol, triglycerides, Creatinine, and urea levels while a decrease in HDL level in comparison to mice normal control group (P<0.05). Serum ALT, cholesterol, triglycerides, creatinine, and urea levels of mice that were administered with walnut oil and methanolic extract ofC. tuberculata at the doses of (1 ml/kg, 2 ml/kg and 3 ml/kg body weight) were significantly lower when compared to toxic control mice group (P<0.05), While HDL level was significantly increased. The significant reduction had also been observed in the levels of serum parameters of mice group, which received standard hepato-protective drug i.e., vitamin C, at the dose of 8 mg/kg body weight (P<0.05). Based on these results, it was evident that liver toxicity caused by the paracetamol administration has recovered toward the normal range by the walnut oil and C. tuberculata extract. Therefore, the present study revealed that (walnut oil andC. tuberculata) exhibit hepatoprotective activities in paracetamol intoxicated mice.

In-Vivo AND In-Silico ANALYSIS OF ANTI- INFLAMMATORY, ANALGESIC, AND ANTI PYRETIC ACTIVITIES OF In-Silico LEAF EXTRACT

“…Therefore, the binding energy of standard drug was also higher than the C. paradisi leaf components such as geraniol and betapinene. The protein TRPV4 is selected as analgesic protein and it is confirmed by previous literature (22,(47)(48)(49)(50)(51)(52)(53). The docking of TRPV4 was done with 2 ligand molecules (geraniol and beta-pinene) and diclofenac is used as standard drug.…”

“…Inflammation in the albino rats' paw was caused by the carrageenan and reduced by the diclofenac and ethanolic leaf extracts of C. paradisi (50,100,200 and 400mg/kg). A total of 36 albino rats were equally divided into three groups (control, standard, experimental).…”

“…In the standard group, rats were treated with diclofenac (100mg/kg), and in the experimental group, rats were treated with the C. paradisi leaf aqueous extract. First, size of the hind paw of rats was measured, followed by injecting the carrageenan doses (50,100,200, and 400mg/kg) into the hind paw at sub-planter region. The carrageenan doses start producing inflammation in the rat's hind paws.…”

Effect of maltodextrin concentration and drying temperature on the physicochemical characteristics of Sacha inchi (Plukenetia volubilis) extract powder

“…Recently these plants have been under keen intrest of various researchers due to their potential health benefits (Zahra et al, 2024;Aziz et al, 2024;Riaz et al, 2023;Waqas et al, 2023;Ahmad et al, 2023a;Ahmad et al, 2023b;Ammara et al, 2023;Aqib et al, 2023;Ejaz et al, 2023;Aziz et al, 2023;Gul et al, 2023). Extracts of various medicinal plants are used in different industries such as pharmaceuticals, food industries and for nanomedicine (Hayat et al, 2023;Hussain et al, 2023;Iram et al, 2023;Riasat et al, 2023;Saleem et al, 2023;Sana et al, 2022;Syed et al, 2023;Zahra et al, 2023;Zawar et al, 2023;Rauf et al, 2023; Effect of maltodextrin concentration and drying temperature on the physicochemical characteristics of Sacha inchi Naveed et al, 2022a;Naveed et al, 202b). Sacha inchi (Plukenetia volubilis L.) is a shrub that grows vines and is commonly known as Sacha beans, mountain beans, or Inka beans (Puangpronpitag et al, 2021).…”

Assessing the pharmacological and biochemical effects of Salvia hispanica (Chia seed) against oxidized Helianthus annuus (sunflower) oil in selected animals

“…Other compounds known for their negative effect on the quality and stability of oils include free fatty acids, unsaponifiable matters, waxes, pigments, solid impurities (mainly fibers), oxidation products (peroxides, aldehydes, ketones, alcohols, and oxidized fatty acids) (Gharby et al, 2016;Aliyar-Zanjani et al, 2019;Said et al, 2022). Several plants contain different chemicals which can be used for treatment of various diseases if they are consumed entirely or in parts with lower cost and less side effects (Sana et al, 2022;Bisht et al, 2021). Sunflower oil is one of the most widely grown essential oils in the world.…”