Hepatoprotective impact of Boldo (Peumus boldus) extract against azoxystrobin induced DNA damage, gene expression modulation, biochemical and histopathological alterations mediated-ROS generation in male rats

Abstract

Peumus boldus Molina, ordinarily mentioned to as 'boldo', is expended in folk medicine to cure gastrointestinal and hepatic diseases. This plant is wealthy in several antioxidant compounds. The current research evaluated the ameliorative effect of boldo leaves extract on lipid peroxidation, liver antioxidant enzymes, ROS generation, DNA damage, gene expression, and histopathological changes induced by Amistar fungicide in male rats. The rats were allocated in four groups treated with boldo extract (50 mg/Kg b.w) and/or with Amistar (1/20 of LD50). As likely, the treatment with fungicide significantly (P<0.05) decreased the antioxidant enzymes activities, while increasing the liver biomarker parameters, lipid peroxidation, ROS generation, DNA damage, and hepatic tissue lesions as well as altered the expression of stress and antioxidant related genes in comparison with control rats. Pretreatment with boldo extract significantly improved the negative effects induced by Amistar on the liver cells. The research results indicate that the boldo extract acts as a defender against the oxidative hepatic injury produced by fungicide. The defensive capability of boldo extract could be owing to the existence of natural antioxidants and anti-inflammatory constituents' likes gallic acid, ellagic acid, querectin, daidzein, ferulic acid and rutin.

Boldo phytochemical and pharmacological activities updated Mini-review

“…In another study leaves phenolic contents were investigated using HPLC analysis. Results showed the presence of gallic acid, chlorogenic acid, caffeic acid, syringic acid, rutin, ellagic acid, ferulic acid, naringenin, daidzein, and quercetin (14). In another study, phenolic contents were investigated using HPLC analysis.…”

“…The liver is one of the most important organs in the body, playing a fundamental role in the regulation of diverse processes, among which the metabolism, secretion, storage, and detoxification of endogenous and exogenous substances are prominent (14). The hepatoprotective activity of Boldo was investigated by measuring lipid peroxidation, liver antioxidant enzymes, reactive oxygen species generation, DNA damage, gene expression, and histopathological changes induced by Amistar fungicide in male rats (14). The results showed that pretreatment with boldo extract increased the antioxidant activities of Superoxide dismutases and catalase as well as elevated the antioxidant genes expression (Glutathione peroxidase and glutathione reductase).…”