Peumus boldus Molina, ordinarily mentioned to as 'boldo', is expended in folk medicine to cure gastrointestinal and hepatic diseases. This plant is wealthy in several antioxidant compounds. The current research evaluated the ameliorative effect of boldo leaves extract on lipid peroxidation, liver antioxidant enzymes, ROS generation, DNA damage, gene expression, and histopathological changes induced by Amistar fungicide in male rats. The rats were allocated in four groups treated with boldo extract (50 mg/Kg b.w) and/or with Amistar (1/20 of LD50). As likely, the treatment with fungicide significantly (P<0.05) decreased the antioxidant enzymes activities, while increasing the liver biomarker parameters, lipid peroxidation, ROS generation, DNA damage, and hepatic tissue lesions as well as altered the expression of stress and antioxidant related genes in comparison with control rats. Pretreatment with boldo extract significantly improved the negative effects induced by Amistar on the liver cells. The research results indicate that the boldo extract acts as a defender against the oxidative hepatic injury produced by fungicide. The defensive capability of boldo extract could be owing to the existence of natural antioxidants and anti-inflammatory constituents' likes gallic acid, ellagic acid, querectin, daidzein, ferulic acid and rutin.