Hepatoprotectivity of Panduratin A against liver damage: In vivo demonstration with a rat model of cirrhosis induced by thioacetamide

Abstract

This experiment evaluated Panduratin A (PA), a chalcone isolated from Boesenbergia rotunda rhizomes, for its hepatoprotectivity. Rats were subjected to liver damage induced by intra-peritoneal injection of thioacetamide (TAA). PA was tested first for its acute toxicity and then administered by oral gavage at doses 5, 10, and 50 mg/kg to rats. At the end of the 8 week, livers from all rats were excised and evaluated ex vivo. Measurements included alkaline phosphatase (AP), alanine transaminase (ALT), aspartate transaminase (AST) and gamma-glutamyl transferase (GGT), serum platelet-derived growth factor (PDGF) and transforming growth factor (TGF-β1), and hepatic metalloproteinase enzyme (MMP-2) and its inhibitor extracellular matrix protein (TIMP-1). Oxidative stress was measured by liver malondialdehyde (MDA) and nitrotyrosine levels, urinary 8-hydroxy 2- deoxyguanosine (8-OH-dG), and hepatic antioxidant enzyme activities. The immunohistochemistry of TGF-β was additionally performed. PA revealed safe dose of 250 mg/kg on experimental rats and positive effect on the liver. The results suggested reduced hepatic stellate cells (HSCs) activity as verified from the attenuation of serum PDGF and TGF-β1, hepatic MMP-2 and TIMP-1, and oxidative stress. The extensive data altogether conclude that PA treatment could protect the liver from the progression of cirrhosis through a possible mechanism inhibiting HSCs activity.

Volatile Oil of Croton Zehntneri Per Oral Sub-Acute Treatment Offers Small Toxicity: Perspective of Therapeutic Use

“…However, the increased levels of GAMA-GT here observed, as related to hepatic toxicity, should be interpreted with caution. First, the range of Gamma-GT normal values is very large (in humans is ≤38 for women and ≤55 for men) and the value obtained in presence of anethole and EOCz is very close to control in other experimental studies in rats (Abdelhalim et al, 2018;Salama et al, 2018). Second, as mentioned above, other blood biochemical biomarkers of liver toxicity were not affected.…”

Hepatoprotective effects of methanolic extract of green tea against Thioacetamide-Induced liver injury in Sprague Dawley rats

“…Therefore, discovery of most effective and less toxic treatment for liver diseases have been increasingly gaining attention. Various natural products have been extensively evaluated for their hepatoprotective role against liver injury caused by different substances ( Salama et al, 2018 , Adewale et al, 2019 , Zhang et al, 2020 , El-Baz et al, 2021 ).…”

Molecular Mechanisms of Antiproliferative Effects of Natural Chalcones

“…Moreover, the anticancer effect of chalcones in experimental animals has also been shown [ 27 , 28 ]. Additionally, in contrast to “classical” anticancer agents, chalcones are well tolerated as documented in animal as well as clinical studies [ 29 , 30 , 31 , 32 , 33 , 34 ].…”