Hepatosteatosis and estrogen increase apolipoprotein O production in the chicken

Schmidinger, Barbara; Weijler, Anna Marianne; Schneider, Wolfgang J.; Hermann, Marcela

doi:10.1016/j.biochi.2016.04.017

Cited by 8 publications

(5 citation statements)

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“…In addition, an interesting gene the apolipoprotein O ( APOO ) around the significant SNPs was also detected, which was a new member of the apolipoprotein family. Previous studies reported that APOO participated in fatty acid and lipid metabolism and regulated the production of lipoprotein through hormones in the chicken [40, 41]. We therefore suggest that the APOO is a pleiotropic gene, which affects not only the fatty deposition, but also the egg number at a specific stage in chickens.…”

Section: Discussionmentioning

confidence: 65%

Genome-wide association analysis of egg production performance in chickens across the whole laying period

et al. 2019

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Background Egg production is the most economically-important trait in layers as it directly influences benefits of the poultry industry. To better understand the genetic architecture of egg production, we measured traits including age at first egg (AFE), weekly egg number (EN) from onset of laying eggs to 80 weeks which was divided into five stage (EN1: from onset of laying eggs to 23 weeks, EN2: from 23 to 37 weeks, EN3: from 37 to 50 weeks, EN4: from 50 to 61 weeks, EN5: from 61 to 80 weeks) based on egg production curve and total egg number across the whole laying period (Total-EN). Then we performed genome-wide association studies (GWAS) in 1078 Rhode Island Red hens using a linear mixed model . Results Estimates of pedigree and SNP-based genetic parameter showed that AFE and EN1 exhibited high heritability (0.51 ± 0.09, 0.53 ± 0.08), while the h2 for EN in other stages varied from low (0.07 ± 0.04) to moderate (0.24 ± 0.07) magnitude. Subsequently, seven univariate GWAS for AFE and ENs were carried out independently, from which a total of 161 candidate SNPs located on GGA1, GGA2, GGA5, GGA6, GGA9 and GGA24 were identified. Thirteen SNP located on GGA6 were associated with AFE and an interesting gene PRLHR that may affect AFE through regulating oxytocin secretion in chickens. Sixteen genome-wide significant SNPs associated with EN3 were in a strong linkage disequilibrium (LD) region spanning from 117.87 Mb to 118.36 Mb on GGA1 and the most significant SNP (rs315777735) accounted for 3.57% of phenotypic variance. Genes POLA1 , PDK3 , PRDX4 and APOO identified by annotating sixteen genome-wide significant SNPs can be considered as candidates associated with EN3. Unfortunately, our study did not find any candidate gene for the total egg number. Conclusions Findings in our study could provide promising genes and SNP markers to improve egg production performance based on marker-assisted breeding selection, while further functional validation is still needed in other populations. Electronic supplementary material The online version of this article (10.1186/s12863-019-0771-7) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 65%

Genome-wide association analysis of egg production performance in chickens across the whole laying period

et al. 2019

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“…Previous studies showed that a MIC26 55kDa was invariably observed [ 43 , 46 , 47 , 53 , 54 , 65 ] and several indications supported the wrong interpretation that this form is a glycosylated variant of MIC26. A cleavage of the presumed MIC26 55kDa into an apparent 22 kDa form was observed after cABC treatment [ 43 , 46 ].…”

Section: Discussionmentioning

confidence: 99%

“…Hence, a dual role of MIC26 as a mitochondrial as well as glycosylated protein emerged into prominence. However, studies in chicken after the discovery of the mitochondrial MIC26 22kDa still focussed exclusively on the so-called MIC26 55kDa secreted version [ 53 , 54 ]. Therefore, it is important to study both the MIC26 22kDa and MIC26 55kDa together.…”

Section: Introductionmentioning

confidence: 99%

MIC26 and MIC27 are bona fide subunits of the MICOS complex in mitochondria and do not exist as glycosylated apolipoproteins

Lubeck,

Derkum,

Naha

et al. 2023

PLoS ONE

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Impairments of mitochondrial functions are linked to human ageing and pathologies such as cancer, cardiomyopathy, neurodegeneration and diabetes. Specifically, aberrations in ultrastructure of mitochondrial inner membrane (IM) and factors regulating them are linked to diabetes. The development of diabetes is connected to the ‘Mitochondrial Contact Site and Cristae Organising System’ (MICOS) complex which is a large membrane protein complex defining the IM architecture. MIC26 and MIC27 are homologous apolipoproteins of the MICOS complex. MIC26 has been reported as a 22 kDa mitochondrial and a 55 kDa glycosylated and secreted protein. The molecular and functional relationship between these MIC26 isoforms has not been investigated. In order to understand their molecular roles, we depleted MIC26 using siRNA and further generated MIC26 and MIC27 knockouts (KOs) in four different human cell lines. In these KOs, we used four anti-MIC26 antibodies and consistently detected the loss of mitochondrial MIC26 (22 kDa) and MIC27 (30 kDa) but not the loss of intracellular or secreted 55 kDa protein. Thus, the protein assigned earlier as 55 kDa MIC26 is nonspecific. We further excluded the presence of a glycosylated, high-molecular weight MIC27 protein. Next, we probed GFP- and myc-tagged variants of MIC26 with antibodies against GFP and myc respectively. Again, only the mitochondrial versions of these tagged proteins were detected but not the corresponding high-molecular weight MIC26, suggesting that MIC26 is indeed not post-translationally modified. Mutagenesis of predicted glycosylation sites in MIC26 also did not affect the detection of the 55 kDa protein band. Mass spectrometry of a band excised from an SDS gel around 55 kDa could not confirm the presence of any peptides derived from MIC26. Taken together, we conclude that both MIC26 and MIC27 are exclusively localized in mitochondria and that the observed phenotypes reported previously are exclusively due to their mitochondrial function.

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“…In particular, the NTSR1 gene affects the anorexic pathway in chickens [47] and was identified as a major differentially expressed gene in a comparative ovarian transcriptome analysis of chickens exhibiting high and low levels of spawning [48]. The DNA polymerase α 1, catalytic subunit (POLA1) gene and the fatty acid and lipid metabolism-related apolipoprotein O (APOO) gene were identified as candidate genes that were strongly associated with weekly egg number [49]. The selective sweep of genes affecting the ovaries and fallopian tubes was confirmed in the KNC population, and this process presumably affected egg-laying and eggshell traits.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Selection Signatures between Korean Native and Commercial Chickens Using 600K SNP Array Data

Cho

Manjula

Kim

et al. 2021

Genes

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Korean native chickens (KNCs) comprise an indigenous chicken breed of South Korea that was restored through a government project in the 1990s. The KNC population has not been developed well and has mostly been used to maintain purebred populations in the government research institution. We investigated the genetic features of the KNC population in a selection signal study for the efficient improvement of this breed. We used 600K single nucleotide polymorphism data sampled from 191 KNCs (NG, 38; NL, 29; NR, 52; NW, 39; and NY, 33) and 54 commercial chickens (Hy-line Brown, 10; Lohmann Brown, 10; Arbor Acres, 10; Cobb, 12; and Ross, 12). Haplotype phasing was performed using EAGLE software as the initial step for the primary data analysis. Pre-processed data were analyzed to detect selection signals using the ‘rehh’ package in R software. A few common signatures of selection were identified in KNCs. Most quantitative trait locus regions identified as candidate regions were associated with traits related to reproductive organs, eggshell characteristics, immunity, and organ development. Block patterns with high linkage disequilibrium values were observed for LPP, IGF11, LMNB2, ERBB4, GABRB2, NTM, APOO, PLOA1, CNTN1, NTSR1, DEF3, CELF1, and MEF2D genes, among regions with confirmed selection signals. NL and NW lines contained a considerable number of selective sweep regions related to broilers and layers, respectively. We recommend focusing on improving the egg and meat traits of KNC NL and NW lines, respectively, while improving multiple traits for the other lines.

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Hepatosteatosis and estrogen increase apolipoprotein O production in the chicken

Cited by 8 publications

References 50 publications

Genome-wide association analysis of egg production performance in chickens across the whole laying period

Genome-wide association analysis of egg production performance in chickens across the whole laying period

MIC26 and MIC27 are bona fide subunits of the MICOS complex in mitochondria and do not exist as glycosylated apolipoproteins

Comparison of Selection Signatures between Korean Native and Commercial Chickens Using 600K SNP Array Data

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