Hepatotoxicity and reproductive disruption in male lizards (Eremias argus) exposed to glufosinate-ammonium contaminated soil

Zhang, Luyao; Diao, Jinling; Chen, Li; Wang, Zikang; Zhang, Wenjun; Li, Yao; Tian, Zhongnan; Zhou, Zhiqiang

doi:10.1016/j.envpol.2018.12.004

Cited by 17 publications

(12 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pesticide residues is a persistent and serious environmental problem. In recent years, an increasing number of studies have focused on the potential health risks of pesticides to nontarget organisms. − Pesticide exposure alters the gut microbiota and metabolic profiles of organisms, leading to serious health risks. ,,, In this study, we found that the chiral pesticide penconazole and its enantiomers influenced intestinal microbiota composition and disrupted metabolic profiles in mice. In addition, the changes caused by penconazole and its enantiomers differed across treatment and control groups.…”

Section: Discussionmentioning

confidence: 63%

Impacts of Penconazole and Its Enantiomers Exposure on Gut Microbiota and Metabolic Profiles in Mice

Meng

Liu

Jia

et al. 2019

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

Exposure to chiral pesticides poses many potential health risks. In this study, we examined the impacts of exposure to penconazole and its enantiomers on gut microbiota and metabolic profiles in mice. The relative abundance of microbiota in cecal content significantly changed following exposure to penconazole and its enantiomers. At the genus level, the relative abundances of seven gut microflora were altered following exposure to (−)-penconazole. Both (±)-penconazole and (+)-penconazole caused significant changes in the relative abundances of five gut microflora. In addition, targeted serum metabolomics analysis showed disturbed metabolic profiles following exposure. Respectively, (±)-penconazole, (+)-penconazole, and (−)-penconazole exposure significantly altered the relative levels of 29, 23, and 36 metabolites. In general, exposure to penconazole and its enantiomers caused disorders in gut microbiota and metabolic profiles of mice. The potential health risks of penconazole and its enantiomers now require further evaluation.

show abstract

Section: Discussionmentioning

confidence: 63%

Impacts of Penconazole and Its Enantiomers Exposure on Gut Microbiota and Metabolic Profiles in Mice

Meng

Liu

Jia

et al. 2019

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The liver is an important detoxification organ in the body, but there are few studies on the toxicity of GLA to the liver. Currently, 7 Also, we found that the structure and function of the liver of zebrafish were damaged after exposure to GLA, indicating that GLA is hepatotoxic for zebrafish.…”

Section: Discussionmentioning

confidence: 93%

“…The dose of GLA for the exposed group was 1.25, 2.5, 5, 10, and 20 mg/L water based on 0.0625%, 0.125%, 0.25%, 0.5%, and 1% of 2000 mg/ kg (the rat transdermal LD50 and birds acute LD50 data). 7,19 The stock solution was prepared by dissolving GLA in distilled water to a concentration of 100 mg/L. The exposure medium was changed every 2 days to keep the concentration of the solution consistent during the experiment.…”

Section: Animals and Treatmentmentioning

confidence: 99%

“…Luyao Zhang's research found that male lizards exposed to GLA-contaminated soil had severe damage to liver and testes, which was associated with oxidative stress. 7 Guanghua Xiong has confirmed that GLA induces immunotoxicity and oxidative stress in zebrafish embryos. 8 To add insult to injury, Lajmanovich found that the GlA-microplastic mixture showed higher ecotoxicity and binding forces than individual treatments.…”

mentioning

confidence: 93%

See 1 more Smart Citation

Glufosinate‐ammonium causes liver injury in zebrafish by blocking the Nrf2 pathway

Kou,

Chen,

Feng

et al. 2023

Environmental Toxicology

View full text Add to dashboard Cite

Glufosinate‐ammonium (GLA) is a widely used herbicide, but less research has been done on its harmful effects on non‐target organisms, especially aquatic organisms. In this study, 600 adult zebrafish were exposed to different concentration of GLA (0, 1.25, 2.5, 5, 10, and 20 mg/L) for 7 days, and the livers were dissected on the eighth day to examine the changes in liver structure, function, oxidative stress, inflammation, apoptosis, and Nrf2 pathway, and finally to clarify the mechanism of GLA induced liver injury in zebrafish. The levels of alanine aminotransferase, aspartate aminotransferase, reactive oxygen species, malondialdehyde, inflammatory factors (IL‐6 and TNF‐α), and caspase‐3 gradually increased, while the levels of superoxide dismutase, catalase, glutathione, and glutathione peroxidase gradually decreased with the increase of GLA concentration. The Nrf2 pathway was activated at low concentrations (1.25–5 mg/L) and significantly inhibited at high concentrations (10 and 20 mg/L). These results suggested that GLA could cause oxidative stress, inflammation, and apoptosis in zebrafish liver. Therefore, GLA can cause liver injury in zebrafish, and at high concentrations, the inhibition of Nrf2 pathway is one of the important causes of liver injury.

show abstract

“…However, emerging evidence suggests its potential toxicity. An in vivo study on lizards observed GLA-induced severe testis lesions by oxidative damage (Zhang et al, 2019), while an in vitro study on human sperm recently revealed its impairment on sperm mitochondria respiration efficiency (Ferramosca et al, 2021), which indicates GLA toxicity on male reproduction. Our previous study (Ma et al, 2021) also showed its effects on male reproductive health with significantly altered DNA methylation, histone H3 lysine 27 trimethylation (H3K27me3) and histone H3 lysine 9 trimethylation (H3K9me3), as well as transcriptome in mouse sperm.…”

mentioning

confidence: 99%

Glufosinate-Ammonium Induced Aberrant Histone Modifications in Mouse Sperm Are Concordant With Transcriptome in Preimplantation Embryos

Fan

Xiao

et al. 2022

Front. Physiol.

View full text Add to dashboard Cite

Glufosinate-ammonium (GLA) is a widely used herbicide with emerging concern over its male reproductive toxicity. Abnormalities in sperm histone modification induced by GLA exposure observed in our previous study aroused our interest in whether such alterations could further affect embryonic gene expression. Here we administered adult male mice with 0.2 mg/kg⋅day of GLA for 5 weeks to collect their sperm or 4-cell embryos after copulation. Cleavage Under Targets and Tagmentation (CUT&Tag) sequencing showed alterations of sperm H3 lysine 4 trimethylation (H3K4me3) and histone H3 lysine 27 acetylation (H3K27ac), which are active histone modification marks involved in embryo development, while RNA sequencing identified differentially expressed genes in 4-cell embryos. Differentially H3K4me3 and H3K27ac occupied regions were mainly distributed at the gene promoters and putative enhancers, and were enriched in pathways related to the immune system and nervous system. Integrative analysis of these sequencing data showed that genes such as Mgl2 with increased H3K4me3 and H3K27ac in sperm were up-regulated in embryos, and vice versa for genes such as Dcn. Additionally, differentially occupied H3K4me3 and H3K27ac in sperm were linked to gene expression changes in both paternal and maternal alleles of 4-cell embryos. In conclusion, GLA-induced changes in sperm H3K4me3 and H3K27ac are concordant with gene expression in preimplantation embryos, which might further affect embryo development and offspring health.

show abstract

Hepatotoxicity and reproductive disruption in male lizards (Eremias argus) exposed to glufosinate-ammonium contaminated soil

Cited by 17 publications

References 38 publications

Impacts of Penconazole and Its Enantiomers Exposure on Gut Microbiota and Metabolic Profiles in Mice

Impacts of Penconazole and Its Enantiomers Exposure on Gut Microbiota and Metabolic Profiles in Mice

Glufosinate‐ammonium causes liver injury in zebrafish by blocking the Nrf2 pathway

Glufosinate-Ammonium Induced Aberrant Histone Modifications in Mouse Sperm Are Concordant With Transcriptome in Preimplantation Embryos

Contact Info

Product

Resources

About