Hepatotoxicity induced by sub-acute exposure of rats to 2,4-Dichlorophenoxyacetic acid based herbicide “Désormone lourd”

Tayeb, Wafa; Nakbi, Amel; Trabelsi, Médiha; Attia, N.; Miled, Abdelhédi; Hammami, Mohamed

doi:10.1016/j.jhazmat.2010.04.018

Cited by 72 publications

(38 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The liver of C. gariepinus exposed to the different concentration of 2,4-D showed distortion of hepatic architecture and extensive damage to hepatocytes, which is consistent with previous reports on the toxicity of xenobiotics such as herbicides and pesticides (Tayeb et al, 2010), metals (Muhammad et al, 2011) and microcystins (Okogwu et al, 2014). The kidney and cardiac cells were also extensively damaged as renal and intra cardiac haemorrhages were observed, which depict loss of blood due to cellular damage and could lead to organ failure.…”

Section: Discussionsupporting

confidence: 89%

See 1 more Smart Citation

Behavioural, Haematological and Histopathological Changes in the African Catfish, <i>Clarias gariepinus</i> Exposed to 2,4- Dichlorophenoxyacetic Acid (2,4-D)

Okogwu¹,

Anionwo²,

Anoke³

et al. 2016

Nig. J. Biotechnol

View full text Add to dashboard Cite

The herbicide, 2,4-D is commonly used to control broad leaf weeds in agriculture and to eliminate aquatic weeds. Run-offs to floodplains that serve as breeding sites of some fish species such as Clarias gariepinus is common. This study was undertaken to evaluate the effects of 2,4-D on the behaviour, haematology and histology of the African catfish, C. gariepinus, in order to understand its toxicity. Juvenile catfish weighing 10.03±14.1g were exposed to lethal concentrations and subsequently three sublethal concentrations (0.43, 0.58 and 0.72mg/L) of 2,4-D. The behaviour of the fish was observed during exposure and after 96h, the blood and tissues from the liver, kidney, fin, brain and gills were collected and analyzed. The result showed that exposed fish displayed signs of asphyxiation. The packed cell volume (PCV), red blood cell (RBC) and haemoglobin (Hb) significantly decreased by 17-40% while white blood cell (WBC) increased significantly (p<0.05) by 78-137% in a concentration dependent pattern. Histopathological analysis showed extensive damage to the liver, kidney, fin, brain and gills of the fish exposed to sublethal concentration, which suggests that 2,4-D causes deleterious harm to hepatocytes and renal, neural, fin and gill cells. Damage to the gill filaments could impair oxygen uptake resulting in asphyxia and consequently death over a period. The study clearly shows that C. gariepinus is highly susceptible to the toxicity of 2,4-D, therefore the use of this herbicide in rice paddies near the breeding sites of the fish should be discouraged.

show abstract

Section: Discussionsupporting

confidence: 89%

“…Blocks were made and sectioned at 5µm thickness using a rotary microtome. Sections were rehydrated in distilled water and stained with Hematoxylin-Eosin (H-E), then examined and micrographed under light microscopy (Tayeb et al 2010).…”

Section: Methodsmentioning

confidence: 99%

Behavioural, Haematological and Histopathological Changes in the African Catfish, <i>Clarias gariepinus</i> Exposed to 2,4- Dichlorophenoxyacetic Acid (2,4-D)

Okogwu¹,

Anionwo²,

Anoke³

et al. 2016

Nig. J. Biotechnol

View full text Add to dashboard Cite

show abstract

“…The authors found that the administration of 1.5 and 3mg/day of 2,4-D during 25 days induced in vivo oxidation. Recently, from the series of experiments described by Tayeb et al (2010;2011 b;2011c) and 2011a;2011b): it is quite evident that subacute exposure of rats to 5, 15, 75 and 150 mg/kg BW during 28 days caused significant negative changes in the erythrocyte, liver and kidney functions. The fatty acid composition of the erythrocyte membranes also of hepatocytes has been altered by the 2,4-D exposure with 2,4-D exposure increased levels of SFA and the decreased level of unsaturated fatty acids (UFA); increase in the index of fatty acid unsaturation.…”

Section: Ratsmentioning

confidence: 99%

“…It is known that it disturbs metabolism (Palmeira et al, 1995). Moreover, immunosuppressive (Pistl et al, 2003), neurotoxic (Bortolozzi et al, 2004) and hepatotoxic effects have been well documented (Tuschl and Schawb, 2003;Tayeb et al, 2010). As a phenoxyherbicide, 2,4-D may cause an array of adverse effects to the nervous system such as myotonia, disruption of the activity of nervous system and behavioral changes (Bortolozzi et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Oxidative Stress Induced by the 2,4-Dichlorophenoxyacetic Herbicide

Tayeb¹,

Nakbi²,

Chaieb³

et al. 2012

Oxidative Stress - Environmental Induction and Dietary Antioxidants

View full text Add to dashboard Cite

“…Its herbicidal activity is mediated by an auxin like capacity to alter normal protein synthesis and cell division in plant meristems and leaves [2,3]. Although it has recently been suggested that its herbicidal activity may also be due to an increase in the production of reactive oxygen species (ROS) leading to the generation of oxidative stress in the weed [4,5].…”

Section: Introductionmentioning

confidence: 99%

Olive oil protects against 2,4‐dichlorophenoxyacetic acid‐induced oxidative renal dysfunction in adult rats

Nakbi

Tayeb

Dabbou

et al. 2012

Euro J Lipid Sci & Tech

Self Cite

View full text Add to dashboard Cite

This study was aimed to evaluate the protective effect of extra virgin olive oil (EVOO) and its fractions (hydrophilic (HF) and lipophilic (LF)) treatment on 2,4-dichlorophenoxiacetic acid (2,4-D)-induced nephrotoxicity in rats. The Wistar rats (n ¼ 80, 210-230 g) were divided into eight groups having ten animals, i.e., control group (C), (2,4-D) group that received 2,4-D (5 mg/kg b.w.), (2,4-D/EVOO) group was treated with 2,4-D plus EVOO, (2,4-D/olive oil hydrophilic fraction, OOHF) group that received 2,4-D plus HF, (2,4-D/olive oil lipophilic fraction, OOLF) group was treated with 2,4-D plus LF, (EVOO) group that received only EVOO, (OOHF) group was given HF and (OOLF) group was treated with the LF. These components were administered daily by gavage for 4 wk. 2,4-D administration affected plasma urea and creatinine levels, which increased while uric acid significantly decreased. A marked increase of malondialdehyde level and with a significant decrease in enzymatic antioxidant's activities, were also observed in 2,4-D treated rats. The co-administration of EVOO and its fractions along with 2,4-D resulted in a reversal of 2,4-D induced biochemical changes in kidney accompanied by a significant decrease in lipid peroxidation and an increase in the level of the antioxidant defence system. Light microscopy investigations revealed that 2,4-D exposure induced numerous histopathological alterations in the kidney. EVOO administration with 2,4-D severely reduced the toxicity of 2,4-D and preserved the normal histological architecture of the renal tissue. However, a milder histopathological improvement was observed in animals co-treated with HF or LF plus 2,4-D. In conclusion, the present study suggest that the nephroprotective potential of EVOO against 2,4-D toxicity might be due to the synergic effect of the two fractions, which could be useful for achieving optimum effects in 2,4-D-induced renal damage.

show abstract

Hepatotoxicity induced by sub-acute exposure of rats to 2,4-Dichlorophenoxyacetic acid based herbicide “Désormone lourd”

Cited by 72 publications

References 52 publications

Behavioural, Haematological and Histopathological Changes in the African Catfish, <i>Clarias gariepinus</i> Exposed to 2,4- Dichlorophenoxyacetic Acid (2,4-D)

Behavioural, Haematological and Histopathological Changes in the African Catfish, <i>Clarias gariepinus</i> Exposed to 2,4- Dichlorophenoxyacetic Acid (2,4-D)

Oxidative Stress Induced by the 2,4-Dichlorophenoxyacetic Herbicide

Olive oil protects against 2,4‐dichlorophenoxyacetic acid‐induced oxidative renal dysfunction in adult rats

Contact Info

Product

Resources

About