Hepatozoon sp. gamonts as an accidental finding in synovial liquid from an injured maned wolf (Chrysocyon brachyurus) in southeastern Brazil

Abstract

A free-living, adult male maned wolf (Chrysocyon brachyurus) was referred to the Governador “Laudo Natel” – FCAV/Unesp veterinary hospital after being found with skin lesions and a fracture on the right pelvic limb, which had to be amputated due to compromised integrity. Around 20 days later, bilateral accentuated swollen on humerus-radius-ulna articulation was observed. The synovial liquid was drained and sent to the laboratory for synovial cytology with Rosenfeld staining that revealed predominantly degenerated neutrophils with karyolytic chromatin associated with intracellular inclusions suggestive of Hepatozoon sp. gametocytes. Blood and synovial liquid samples were submitted to molecular analysis, aiming to amplify the Hepatozoon spp. 18S rRNA gene fragment. Despite the positioning of the found Hepatozoon sequence together with Hepatozoon canis previously detected in domestic carnivores, the BLAST analysis showed only 98% identity with H. canis. To the best of the authors’ knowledge, this is the first time a Hepatozoon was detected in the synovial liquid by clinical pathology and molecular analyses.

Molecular Detection of Tick-Borne Agents in Cats from Southeastern and Northern Brazil

“…Hepatozoon sp. DNA detected in a naturally infected maned-wolf (Chrysocyon brachyurus) was used as a positive control [71]. Sterile ultrapure water (Nuclease-Free Water, Promega Corporation) was used as a negative control.…”

Hematological, clinical and epidemiological aspects of Hepatozoon canis infection by parasitological detection in dogs from the rural area of Sousa, Paraíba, Brazil

“…Direct blood smear research has been described as highly specific, but with low sensitivity (RAMOS et al, 2010;O'DWYER, 2011;ROTONDANO et al, 2015;PERLES et al, 2019).…”

Co-infection by multiple vector-borne agents in wild ring-tailed coatis (Nasua nasua) from Iguaçu National Park, southern Brazil

“…(ITS—23S–5S) ITS2F ITS2R Characterization cPCR 5′-AGGATCTGACTCTAGTAACGAG-3′ 5′-CTCCCATGTCTTAAGACAAAG-3′ 300 94 °C for 2 min, 35 cycles; 94 °C for 30 s, 58 °C for 30 s, 72 °C for 1 min 72 °C for 5 min 26 Anaplasma spp. ( gltA gene) External primers F4b R1b EHR-C5136F HER-778R Characterization nPCR 5′-CCGGGTTTTATGTCTACTGC-3′ 5′-CGATGACCAAAACCCAT-3′ 5′-TTYATGTCYACTGCTGCKTG-3′ 5′-GCNCCMCCATGMGCTGG-3′ 700 95 °C for min; 35 cycles: 95 °C for 30 s, 55 °C for 30 s and 72 °C for 30 s and a final extension 72 °C for 10 min 27 Ehrlichia spp. ( dsb gene) dsb-330 dsb-728 Screening cPCR 5′-GATGATGTCTGAAGATATGAAACAAAT-3′ 5′-CTGCTCGTCTATTTTACTTCTTAAAGT-3′ 409 95 °C for 2 min; 50 cycles: 95 °C for 15 s, 58 °C for 30 s and 72 °C For 30 s and a final extension 72 °C for 5 min 28 Neorickettsia ristici (16S rRNA gene) ER3-F ER2-R ER 3a-F ER2a-R Screening nPCR 5′-ATTTGAGAGTTTGATCCTGG-3′ 5′-GTTTTAAATGCAGTTCTTGG-3′ 5′-CTAGCGGTAGGCTTAAC-3′ 5′-CACACCTAACTTACGGG-3′ 450 94 ºC for 5 min; 30 cycles: 94 ºC for 1 min, 60 ºC for 2 min and 72 ºC for 1 min, and a final extension 72 ºC for 7 min 29 Bartonella spp.…”

“…DNA from Hepatozoon sp. detected in a naturally infected maned-wolf ( Chrysocyon brachyurus ) was used as positive control 27 . For Piroplasms screening, Babesia vogeli DNA (Jaboticabal strain) were used as a positive control.…”