Herb medicine Yin-Chen-Hao-Tang ameliorates hepatic fibrosis in bile duct ligation rats

Lee, Tzung-Yan; Chang, Hen‐Hong; Chen, Jenn‐Han; Hsueh, Ming-Lung; Kuo, Jong-Jen

doi:10.1016/j.jep.2006.07.042

Cited by 88 publications

(63 citation statements)

References 30 publications

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“…21) Liver samples were homogenized and hydrolyzed in 6 M HCl for measurement of hydroxyproline content. Protein concentration was determined by the Bradford reagent (Bio-Rad) using bovine serum albumin as the standard.…”

Section: Determination Of Serum Levels Of Hyaluronic Acid (Ha) Type mentioning

confidence: 99%

Combination Therapy with Taurine, Epigallocatechin Gallate and Genistein for Protection against Hepatic Fibrosis Induced by Alcohol in Rats

Zhuo

Liao

et al. 2012

Biological & Pharmaceutical Bulletin

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This study was to investigate the possibility of enhancing the anti-fibrotic effect by using a combination therapy with taurine, epigallocatechin gallate and genistein in a rat liver fibrosis model induced by alcohol, and to explore its underlying mechanism. Hepatic fibrosis was induced by intragastric administration with various amount of alcohol (5.0-9.5 g/kg) within 24 weeks in rats. The model group received alcohol only, and treatment groups received the corresponding drugs plus alcohol respectively, while the normal control group received an equal volume of saline. The antifibrotic effects of combination therapy were assessed directly by hepatic histology, and indirectly by measurement of serum biochemical markers, the fibrosis markers and related key cytokines/proteins. The results showed that combination therapy could significantly improve the liver function, as indicated by decreasing levels of alanine transaminase, aspartate transaminase, alkaline phosphatase, γ-glutamyltransferase, interleukin-6 and tumor necrosis factor-α. Moreover, combination therapy could effectively suppress the serum levels of fibrosis markers and hepatic hydroxyproline content, inhibit collagen deposition and reduce the pathological tissue damage. Research on mechanism showed that combination therapy was able to markedly reduce lipid peroxidation and recruit the anti-oxidative defense system, and inhibit the expression of B-cell lymphoma 2, α-smooth muscle actin, transforming growth factor β 1 and small mothers against decapentaplegic homolog 3 proteins. Our results showed that combination therapy is effective in attenuating hepatic injury and fibrosis in the alcohol-induced rat model. The improved efficacy of the combination therapy with its good safety profile could represent a new protective approach for liver fibrosis.

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Section: Determination Of Serum Levels Of Hyaluronic Acid (Ha) Type mentioning

confidence: 99%

Combination Therapy with Taurine, Epigallocatechin Gallate and Genistein for Protection against Hepatic Fibrosis Induced by Alcohol in Rats

Zhuo

Liao

et al. 2012

Biological & Pharmaceutical Bulletin

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“…This decoction has long been used in Taiwan and China as an anti-inflammatory, antipyretic, and choleretic agent for liver disorders and jaundice. A variety of biological effects caused by YCHT have been described including potent effects on hepatic fibrosis (11,12) and hepatic apoptosis both in vitro (13) and in vivo (14,15). In addition, upregulation of ApoA-I in bile duct ligation rats after YCHT administration indicated enhanced triglyceride metabolism, which may link ApoA-I deficiency to the pathophysiology of liver fibrosis (15).…”

Section: Introductionmentioning

confidence: 99%

Alleviation of hepatic oxidative stress by Chinese herbal medicine Yin-Chen-Hao-Tang in obese mice with steatosis

Lee

Chang²,

Lo³

et al. 2010

Int J Mol Med

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Abstract. Obesity is associated with a complex systemic inflammatory state that has been implicated in the development of hepatic steatosis. This study was to test the efficacy of Yin-Chen-Hao-Tang (YCHT), an agent that improves hepatic triglyceride metabolism in its ability to modulate pathways implicated in hepatic steatosis. Mice were fed with high-fat diet for fifteen weeks. The therapeutic mechanism of YCHT likely enhanced adiponectin and endothelial progenitor cells, and up-regulation of peroxisome proliferator-activated receptor Á might be responsible for fatty liver diseases. In addition, YCHT anti-oxidative stress effect might be associated with inhibition of hepatic free fatty acid concentrations and elevation of the glutathione levels in hepatic tissues. Furthermore, YCHT action mechanisms might promote senescence marker protein-30 metabolism that increase resistance to hepatic oxidative stress. These findings suggest a novel therapeutic approach for fatty liver progression in obesity mice.

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“…The failure of bile salt excretion in cholestasis leads to retention of hydrophobic bile salts within the hepatocytes and causes apoptosis and necrosis (Lee et al, 2007). Bile duct ligation produces the lipid peroxidation in cellular membranes that induces cell injury and decreased in glutathione indicating importance of free radical which damages the defense system in body.…”

Section: Introductionmentioning

confidence: 99%

Hepatoprotective effect of Cocculus hirsutus on bile duct ligation-induced liver fibrosis in Albino Wistar rats

Thakare

Jain

Patil

et al. 2009

Bangladesh J Pharmacol

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In this animal model (Wistar rats of either sex), common bile duct was ligated for 28 days. Rats were treated for 28 days with methanol extract of Cocculus hirsutus. On day 29, blood and liver were collected for biochemical estimation and histopathological studies. Bile duct ligation produced liver fibrosis with generation of reactive oxygen species and induction of oxidative stress. Hence the different concentrations of methanolic extract of C. hirsutus were evaluated for in vivo glutathione reductase activity. On bile duct ligation the liver fibrosis was induced with significant rise in serum marker enzymes levels. The hydroxyproline accumulation caused by hydrophilic bile acids accompanied by elevated hepatic lipid peroxidation, and glutathione levels. Treatment with C. hirsutus extract decreased the elevated levels of serum marker enzymes showing hepatoprotection, which was further confirmed by histopathological results. Article Info

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Herb medicine Yin-Chen-Hao-Tang ameliorates hepatic fibrosis in bile duct ligation rats

Cited by 88 publications

References 30 publications

Combination Therapy with Taurine, Epigallocatechin Gallate and Genistein for Protection against Hepatic Fibrosis Induced by Alcohol in Rats

Combination Therapy with Taurine, Epigallocatechin Gallate and Genistein for Protection against Hepatic Fibrosis Induced by Alcohol in Rats

Alleviation of hepatic oxidative stress by Chinese herbal medicine Yin-Chen-Hao-Tang in obese mice with steatosis

Hepatoprotective effect of Cocculus hirsutus on bile duct ligation-induced liver fibrosis in Albino Wistar rats

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