Conventional medicine is any agent that largely used by most healthcare professionals to treat any disease that infects humans and animals or to decrease the severity of any condition or diagnosis or prevention. 1 Herbal medicine considered to be the oldest form of healing and treating different diseases. The history of herbal medicine is part of the history of medicine itself and herbal knowledge came to Europe from the Middle East during the crusades. Herbal medicine is classified as 'complementary' or 'alternative' in most countries, it stills the only type of medicine that widely available to much of the world's population. Herbal medicines have been utilized in most traditional cultures and have had a vital impact on many systems, including Ayurvedic (Indian) medicine, traditional Chinese medicine, and conventional medicine. There was a written record of Egyptian, Roman, Persian and Hebrew cultures reported that they were used herbs to cure every complaint and diseases. 2 Although herbal medicine is classed as 'alternative' or 'complementary' in most Western countries, it remains the only form of medicine widely available to much of the world's population. Although herbal medicine is classed as 'alternative' or 'complementary' in most Western countries, it remains the only form of medicine widely available to much of the world's population. 3 These medicines play a major part in the pharmaceutical market in all world contourites. Many of these products are currently sold in pharmaceutical forms such as tablets, capsules, syrups, etc.… prescribed by physicians or over the counter for curing different conditions. It became a good business for several companies to produce and market safe and effective herbal preparations. Most patients go to complementary medicines produced from a natural herbal source because they believe that they are safer, more effective and have fewer side effects than traditional synthetic medicine. In the other hand, consumers believed ABSTRACT Background: There has been a universal increase in the use of herbal medicine products (HMPs). Herbal medicine considered to be the oldest form of healing and treating different diseases. Objective: The aim of this study is to determine the prevalence, attitude of HMPs use and awareness among pharmaceutical practitioner in Jordan. Methods: A descriptive cross-sectional survey was conducted using a self-administered questionnaire on a sample of 230 Jordanian pharmacy practitioner (pharmacist and pharmacy assistant), within 90 different private pharmacies in different cities of Jordan during the period of 6 months in the mid of 2018. The survey questionnaire included: the socio-demographic characteristics of participants, the source of information of the use of the HMPs, the attitude towards the use and safety of the HMPs and their combination with conventional drugs. Results: Out of 230 pharmacy practitioners interviewed in this study, the utilization rate of the HMPs use was just 41.7%. A large number of practitioners (58.3%) preferred conventiona...