Herbal Preparation (Bromelain, Papain, Curcuma, Black Pepper) Enhances Mineralization and Reduces Glucocorticoid-Induced Osteoporosis in Zebrafish

Carnovali, Marta; ramoni, gina; Banfi, Giuseppe; Mariotti, Massimo

doi:10.3390/antiox10121987

Cited by 5 publications

(2 citation statements)

References 44 publications

(53 reference statements)

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“…Carnovali et al tested liquiritigenin in zebrafish, revealing increased bone formation rates in a dose-dependent manner and reduced TRAP staining in zebrafish scales of glucocorticoid-induced OP [ 159 ]. A similar approach was used to analyze natural foods with antioxidant properties (curcuma, papain, bromelain and black pepper), which showed anabolic effects during development and reduced osteoclastic activity in zebrafish scales [ 160 ]. Zebrafish have been of interest to also test marine alkaloids [ 161 ], which altogether shall boost the discovery of potential alternative therapeutics.…”

Section: Zebrafish As Animal Models To Accelerate Discoveries Of Huma...mentioning

confidence: 99%

Functional Validation of Osteoporosis Genetic Findings Using Small Fish Models

Kague

Karasik

2022

Genes

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The advancement of human genomics has revolutionized our understanding of the genetic architecture of many skeletal diseases, including osteoporosis. However, interpreting results from human association studies remains a challenge, since index variants often reside in non-coding regions of the genome and do not possess an obvious regulatory function. To bridge the gap between genetic association and causality, a systematic functional investigation is necessary, such as the one offered by animal models. These models enable us to identify causal mechanisms, clarify the underlying biology, and apply interventions. Over the past several decades, small teleost fishes, mostly zebrafish and medaka, have emerged as powerful systems for modeling the genetics of human diseases. Due to their amenability to genetic intervention and the highly conserved genetic and physiological features, fish have become indispensable for skeletal genomic studies. The goal of this review is to summarize the evidence supporting the utility of Zebrafish (Danio rerio) for accelerating our understanding of human skeletal genomics and outlining the remaining gaps in knowledge. We provide an overview of zebrafish skeletal morphophysiology and gene homology, shedding light on the advantages of human skeletal genomic exploration and validation. Knowledge of the biology underlying osteoporosis through animal models will lead to the translation into new, better and more effective therapeutic approaches.

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Section: Zebrafish As Animal Models To Accelerate Discoveries Of Huma...mentioning

confidence: 99%

Functional Validation of Osteoporosis Genetic Findings Using Small Fish Models

Kague

Karasik

2022

Genes

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“…The treatment of alendronate, an FDA-approved bisphosphonate anti-resorptive drug, alleviates the impact of prednisolone on the scale by decreasing the Trap activity and increasing the Alp activity[ 51 ]. Recent studies have adopted this in vivo scale GIOP model to evaluate the curative effect of the herbal mixture or compounds on the 9 months old zebrafish by treating them with 80 μM prednisolone for 14 days[ 53 54 ].…”

Section: Odeling Glucocorticoid-induced Osteoporosis In Adult Zebrafishmentioning

confidence: 99%

Zebrafish models for glucocorticoid-induced osteoporosis

Lin

Dharini

Peng

et al. 2022

Tzu Chi Medical Journal

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Glucocorticoid-induced osteoporosis (GIOP) is the most common form of secondary osteoporosis due to excessive or long-term glucocorticoid administration, disturbing the homeostasis between bone formation and bone resorption. The bone biology of zebrafish shares a high degree of similarities with mammals. In terms of molecular level, genes and signaling pathways related to skeletogenesis are also highly correlated between zebrafish and humans. Therefore, zebrafish have been utilized to develop multiple GIOP models. Taking advantage of the transparency of zebrafish larvae, their skeletal development and bone mineralization can be readily visualized through in vivo staining without invasive experimental handlings. Moreover, the feasibility of using scales or fin rays to study bone remodeling makes adult zebrafish an ideal model for GIOP research. Here, we reviewed current zebrafish models for GIOP research, focused on the tools and methods established for examining bone homeostasis. As an in vivo , convenient, and robust model, zebrafish have an advantage in performing high-throughput drug screening and could be used to investigate the action mechanisms of therapeutic drugs.

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