Herbal supplement Kamishimotsuto augments resistance exercise-induced mTORC1 signaling in rat skeletal muscle

Kido, Kohei; Sato, Koji; Makanae, Yuhei; Ato, Satoru; Hayashi, Tadahiro; Fujita, Satoshi

doi:10.1016/j.nut.2015.06.015

Cited by 13 publications

(16 citation statements)

References 26 publications

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“…Furthermore, it has been suggested that activation of the AGEs/RAGE pathway decreases protein synthesis by deactivating the mTOR pathway . Ribosomal protein S6 is located downstream of the mTOR pathway and is one of the indicators of mTOR pathway activation . In the present study, expression of p‐rpS6 decreased in the DM group.…”

Section: Discussionsupporting

confidence: 51%

Effects of combined treatment with blood flow restriction and low‐intensity electrical stimulation on diabetes mellitus‐associated muscle atrophy in rats

Tanaka

Morifuji

Yoshikawa

et al. 2018

Journal of Diabetes

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Section: Discussionsupporting

confidence: 51%

Effects of combined treatment with blood flow restriction and low‐intensity electrical stimulation on diabetes mellitus‐associated muscle atrophy in rats

Tanaka

Morifuji

Yoshikawa

et al. 2018

Journal of Diabetes

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“…; Kido et al. ). With voluntary contraction, force production is controlled by neural activity, and a difference in contraction modes affects the muscle activation level (Tesch et al.…”

Section: Discussionmentioning

confidence: 97%

“…In this study, we assessed the effect of differences in contraction mode on the activity of mTORC1 and upstream regulators with consideration for the force-time integral. In order to define the force-time integral, we modified a previously used percutaneous electrical muscle contraction experimental model for animals that had been built with the capacity to execute the three different contraction modes (Ogasawara et al 2013b;Kido et al 2016). With voluntary contraction, force production is controlled by neural activity, and a difference in contraction modes affects the muscle activation level (Tesch et al 1990;Babault et al 2001;Beltman et al 2004).…”

Section: Discussionmentioning

confidence: 99%

“…The nonexercised left gastrocnemius muscle served as the internal control (CON). We previously reported that the percutaneous electrical stimulation of the gastrocnemius muscle increases mTORC1 activation with concomitant increase in muscle protein synthesis (Ogasawara et al 2014;Kido et al 2016). Moreover, 18 exercise sessions induced muscle hypertrophy (Ogasawara et al 2013a).…”

Section: Resistance Exercise Protocolsmentioning

confidence: 98%

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Contraction mode itself does not determine the level of mTORC1 activity in rat skeletal muscle

et al. 2016

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Resistance training with eccentric contraction has been shown to augment muscle hypertrophy more than other contraction modes do (i.e., concentric and isometric contraction). However, the molecular mechanisms involved remain unclear. The purpose of this study was to investigate the effect of muscle contraction mode on mammalian target of rapamycin complex 1 (mTORC1) signaling using a standardized force‐time integral (load (weight) × contraction time). Male Sprague–Dawley rats were randomly assigned to three groups: eccentric contraction, concentric contraction, and isometric contraction. The right gastrocnemius muscle was exercised via percutaneous electrical stimulation‐induced maximal contraction. In experiment 1, different modes of muscle contraction were exerted using the same number of reps in all groups, while in experiment 2, muscle contractions were exerted using a standardized force‐time integral. Muscle samples were obtained immediately and 3 h after exercise. Phosphorylation of molecules associated with mTORC1 activity was assessed using western blot analysis. In experiment 1, the force‐time integral was significantly different among contraction modes with a higher force‐time integral for eccentric contraction compared to that for other contraction modes (P < 0.05). In addition, the force‐time integral was higher for concentric contraction compared to that for isometric contraction (P < 0.05). Similarly, p70S6K phosphorylation level was higher for eccentric contraction than for other modes of contraction (P < 0.05), and concentric contraction was higher than isometric contraction (P < 0.05) 3 h after exercise. In experiment 2, under the same force‐time integral, p70S6K (Thr389) and 4E‐BP1 phosphorylation levels were similar among contraction modes 3 h after exercise. Our results suggest that mTORC1 activity is not determined by differences in muscle contraction mode itself. Instead, mTORC1 activity is determined by differences in the force‐time integral during muscle contraction.

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“…It also maintained the expression of mitochondria-related transcription factors and suppressed the expression of TNF-α in senescence-accelerated mice 21 . In addition, it further augmented resistance to exercise-induced muscle protein synthesis via the mTORC1 signaling pathway 22 . …”

Section: Discussionmentioning

confidence: 99%

Jaeumganghwa-Tang, a traditional herbal formula, improves muscle function and attenuates muscle loss in aged mice

Lee

Kim

2017

JENB

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[Purpose]Jaeumganghwa-Tang (JGT), a traditional herbal formula composed of 12 medicinal herbs, is used for the treatment of age-related diseases. In the present study, we investigated the effects of JGT on muscle mass and function in aged mice. [Methods]Young (5-month-old) and old (19-month-old) male C57BL/6 mice were divided into two groups each; one group received JGT (75 mg/d) and the other group received the vehicle for 6 weeks. At the end of the experimental period, muscle strength was examined using the wire hang test, and the tibialis anterior and gastrocnemius muscles were weighed. Muscle samples were further used for histological analysis to assess muscle damage, and the expression of transforming growth factor-beta was investigated via western blotting and immunohistochemistry. [Results]Our results showed that treatment of old mice with JGT improved muscle strength, increased skeletal muscle mass, alleviated muscle damage, and suppressed intramuscular expression of transforming growth factor-beta. [Conclusion]In conclusion, JGT has beneficial effects on age-related loss of muscle mass and function. Thus, it might serve as a potential therapeutic agent for sarcopenia.

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Herbal supplement Kamishimotsuto augments resistance exercise-induced mTORC1 signaling in rat skeletal muscle

Cited by 13 publications

References 26 publications

Effects of combined treatment with blood flow restriction and low‐intensity electrical stimulation on diabetes mellitus‐associated muscle atrophy in rats

Effects of combined treatment with blood flow restriction and low‐intensity electrical stimulation on diabetes mellitus‐associated muscle atrophy in rats

Contraction mode itself does not determine the level of mTORC1 activity in rat skeletal muscle

Jaeumganghwa-Tang, a traditional herbal formula, improves muscle function and attenuates muscle loss in aged mice

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