Herbicides induce change in metabolic and genetic diversity of bacterial community from a cold oligotrophic lake

Aguayo, Paulina; González, Carlos; Barra, Ricardo; Becerra, José; Martı́nez, Miguel Ángel

doi:10.1007/s11274-013-1530-y

Cited by 12 publications

(7 citation statements)

References 39 publications

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“…[30] recently demonstrated that nitrogen fertilization altered within-habitat bacterial community composition, the abundance of genes involved in key subsystems, and the community catabolic ability. Similar effects have been observed under experimental and field addition of herbicide [31] because target enzymes and pathways of insecticides or herbicides may also be present in microbes.…”

Section: Landscape-level Impacts Of Agriculture On Microbial Communitiessupporting

confidence: 67%

Microbes in the Anthropocene: spillover of agriculturally selected bacteria and their impact on natural ecosystems

Bell

Tylianakis

2016

Proc. R. Soc. B.

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Soil microbial communities are enormously diverse, with at least millions of species and trillions of genes unknown to science or poorly described. Soil microbial communities are key components of agriculture, for example, in provisioning nitrogen and protecting crops from pathogens, providing overall ecosystem services in excess of $1000bn per year. It is important to know how humans are affecting this hidden diversity. Much is known about the negative consequences of agricultural intensification on higher organisms, but almost nothing is known about how alterations to landscapes affect microbial diversity, distributions and processes. We review what is known about spatial flows of microbes and their response to land-use change, and outline nine hypotheses to advance research of microbiomes across landscapes. We hypothesize that intensified agriculture selects for certain taxa and genes, which then ‘spill over’ into adjacent unmodified areas and generate a halo of genetic differentiation around agricultural fields. Consequently, the spatial configuration and management intensity of different habitats combines with the dispersal ability of individual taxa to determine the extent of spillover, which can impact the functioning of adjacent unmodified habitats. When landscapes are heterogeneous and dispersal rates are high, this will select for large genomes that allow exploitation of multiple habitats, a process that may be accelerated through horizontal gene transfer. Continued expansion of agriculture will increase genotypic similarity, making microbial community functioning increasingly variable in human-dominated landscapes, potentially also impacting the consistent provisioning of ecosystem services. While the resulting economic costs have not been calculated, it is clear that dispersal dynamics of microbes should be taken into consideration to ensure that ecosystem functioning and services are maintained in agri-ecosystem mosaics.

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Section: Landscape-level Impacts Of Agriculture On Microbial Communitiessupporting

confidence: 67%

Microbes in the Anthropocene: spillover of agriculturally selected bacteria and their impact on natural ecosystems

Bell

Tylianakis

2016

Proc. R. Soc. B.

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“…2018). Other studies reported the effect of pure 2,4-D on microbial communities (Kobraei and White, 1996; Aguayo et al., 2014; Lozano et al., 2018), but little is known about the collateral impact of commercial formulations of this herbicide on wild biota in agricultural landscapes. These commercial formulations consist of the active ingredient (2,4-D), water and inert elements such as solvents, surfactants and humectants of unknown composition which increase plant cuticle permeability to 2,4-D (Lanctôt et al., 2014).…”

Section: Introductionmentioning

confidence: 99%

“…(<40 μg L −1 ) modify the structure of the bacterial community from a shallow aquifer, leading to an increase in 2,4-D degrading bacteria. In addition, an increase in Actinobacteria growth was detected at 20 mg L −1 of 2,4-D in an oligotrophic lake (Aguayo et al., 2014), which resulted from the ability of Arthrobacter sp. to use 2,4-D and other aromatic compounds as sources of carbon and energy (Sandmann and Loos, 1988; Westerberg et al., 2000).…”

Section: Introductionmentioning

confidence: 99%

Turbidity matters: differential effect of a 2,4-D formulation on the structure of microbial communities from clear and turbid freshwater systems

Lozano

Miranda

Vinocur

et al. 2019

Heliyon

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We evaluated the effect of AsiMax 50®, a commercial formulation of 2,4-D (2,4-dichlorophenoxyacetic acid), on the structure of both micro + nano phytoplankton (>2 μm; species composition and abundance) and cytometric populations (photosynthetic picoplankton (PPP, 0.2–2 μm), which included prokaryotic phycocyanin-rich picocyanobacteria (PC-Pcy), phycoerythrin-rich picocyanobacteria (PE-Pcy) and eukaryotic phototrophs (PEuk); and bacterioplankton (HB), heterotrophic bacteria), using a microcosms-based approach and a single 7-day exposure. Assays were performed on two different microbial assemblages sampled from freshwater bodies of two contrasting turbidity status: clear (chlorophyll a = 7.6 μgL -1 , turbidity = 1 NTU) and organic turbid systems (chlorophyll a = 25.0 μgL -1 , turbidity = 9 NTU). For each system, the herbicide was applied to 500 mL-Erlenmeyer flasks, at seven concentration levels of the active ingredient (a.i.): 0 (control = no addition), 0.02, 0.2, 2, 20, 200 and 2,000 mg a.i.L −1 . The impact of AsiMax 50® seemed to be greater in the turbid system. In this system, total abundance of living (live) micro + nano phytoplankton showed a significant increase at lower concentrations and data were fitted to a humped-shaped curve. For both clear and organic turbid systems, micro + nano phytoplankton decreased in species richness and abundance at higher herbicide concentrations. These results suggest that 2,4-D may mimic hormonal function. Some species, such as Ochromonas sp. and Chlamydomonas sp., showed different responses to herbicide exposure between water systems. In the turbid system, the increase in abundance of the PPP fraction observed at 7-d exposure was probably due to either an increase in PE-Pcy (thus suggesting the existence of auxin pathways) or a reduction in competitive pressure by micro + nano plankton. Our results provide some evidence of the importance of using community-scale approaches in ecotoxicological studies to predict changes in freshwater ecosystems exposed to a 2,4-D-based formulation. However, caution must be taken when extrapolating these effects to real scenarios, as assays were based on a laboratory microcosm experiment.

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“…Nucleic acid-based molecular methods have higher potential to give detailed information on the structure and diversity of microbial communities and specific phylogenetic or functional groups. Thus, investigations of pesticide effects on aquatic microbial assemblages have employed denaturing or temperature gradient gel electrophoresis, terminal restriction fragment length polymorphism, fluorescence in situ hybridisation and, more recently, 454 pyrosequencing (Chinalia and Killham, 2006;Pesce et al, 2006;Stachowski-Haberkorn et al, 2008;Pesce et al, 2009;Tadonléké et al, 2009;Vercraene-Eairmal et al, 2010;Lin et al, 2012;Aguayo et al, 2014;Dimitrov et al, 2014).…”

Section: Investigating Community-level Properties Of Sediment Microormentioning

confidence: 99%

Scientific Opinion on the effect assessment for pesticides on sediment organisms in edge‐of‐field surface water

2015

EFS2

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The EFSA Panel on Plant Protection Products and their Residues (PPR Panel) was tasked to revise the Guidance Document (GD) on Aquatic Ecotoxicology under Council Directive 91/414/EEC (SANCO/3268/2001 rev. 4 (final), 17 October 2002. This scientific opinion of the PPR Panel is the second of three requested deliverables within this mandate. The scientific background for the risk assessment on sediment organisms in edge-of-field surface waters is provided, with reference to benthic ecology and ecotoxicology, available test protocols and current knowledge on exposure and effects of sediment-bound plant protection products (PPPs). The scientific opinion provides approaches on how to derive regulatory acceptable concentrations (RACs) for sediment organisms and exposure to active substances of PPPs and transformation products of these substances, and how to link them in a tiered approach to predicted environmental concentrations (PECs) for the sediment compartment. A list of uncertainties in relation to such approaches is given. SUMMARYSediment Environmental Risk Assessment (ERA) is a combination of exposure and effect assessment. Proposals for these assessments are provided. The opinion describes specific protection goals (SPGs) for sediment-inhabiting organisms based on two options (1) the ecological threshold option (ETO), accepting negligible population effects only, and (2) if applicable, the ecological recovery option (ERO), accepting some population-level effects if ecological recovery takes place within an acceptable time period.Triggers for sediment ecotoxicity testing are proposed together with a decision scheme for when additional testing may be required. The ecotoxicologically relevant concentrations (ERCs) for sediment organisms are proposed, as this will be influenced by the choice of sediment layer, exposure metric and test duration.The current Forum for the co-ordination of pesticide fate models and their use (FOCUS) methodology for surface water does not consider the effect of multi-year applications, which could possibly lead to accumulation of pesticides in sediment. To account for this deficit, a proposed methodology for introducing an accumulation factor that can be used until an updated FOCUS methodology becomes available is presented in this opinion. The accumulation factor can be considered a conservative approach, since it does not include any possible transport processes, such as leaching or volatilisation, which may reduce the accumulation in sediment in the real field situation.A tiered effect assessment approach is proposed for different sediment organisms and how to link regulatory acceptable concentrations (RACs) to predicted environmental concentration (PECs). The assessment of bioaccumulation, biomagnification and secondary poisoning is discussed, as well as the prospect of improving ERA for sediment microorganisms.Transformation products from active substances also may need to be assessed, as well as mixture toxicity of formulations of plant protection products (PPPs). The op...

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Herbicides induce change in metabolic and genetic diversity of bacterial community from a cold oligotrophic lake

Cited by 12 publications

References 39 publications

Microbes in the Anthropocene: spillover of agriculturally selected bacteria and their impact on natural ecosystems

Microbes in the Anthropocene: spillover of agriculturally selected bacteria and their impact on natural ecosystems

Turbidity matters: differential effect of a 2,4-D formulation on the structure of microbial communities from clear and turbid freshwater systems

Scientific Opinion on the effect assessment for pesticides on sediment organisms in edge‐of‐field surface water

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