Herd-specific strains of Mycoplasma bovis in outbreaks of mycoplasmal mastitis and pneumonia

Evaluation of PCR electrospray-ionization mass spectrometry for rapid molecular diagnosis of bovine mastitis

“…However, molecular methods have been shown to higher detection potential than culture (Koskinen et al, 2009;Koskinen et al, 2010). We also show that the rapid detection of barely cultivatable and slow-growing mastitis pathogens, such as M. bovis, by PCR/ESI-MS represents a major advantage for veterinarians in preventing the spread of the disease within respective herds (Aebi et al, 2012). Indeed, the overall workflow for PCR/ESI-MS from receipt of a sample in the laboratory to providing organism identification requires approximately 8 h (Wolk et al, 2012).…”

Loss of diversity within Mycoplasma bovis isolates collected in France from bovines with respiratory diseases over the last 35 years

“…Several authors suggested that this strain heterogeneity was associated with a high level of cattle trading. In contrast, isolates shown to share identical or similar subtypes were in most cases epidemiologically related (same breeding area, same animal or isolation year) (Aebi et al, 2012;Amram et al, 2013;Arcangioli et al, 2012;Hendrick et al, 2013). Recently, a broad infection across the entire Austrian Alps has been reported, suggesting the extensive spread of a single M. bovis clone .…”

“…Six isolates recently collected in Switzerland (Aebi et al, 2012) and the DNA from 3 Austrian strains were also included in the study for comparison.…”

The immune response of bovine mammary epithelial cells to live or heat-inactivated Mycoplasma bovis

“…The S. aureus strain 1904 was used as a positive control (Zbinden et al, 2014) and proved responsiveness of the cultures used. M. bovis strain JF4278 used in the present study represents a Swiss field strain that was isolated from severe mastitis in 2008, when M. bovis mastitis outbreaks started to emerge in Switzerland (Aebi et al, 2012). In parallel, bMEC were challenged with the type strain PG45 that was isolated in 1961 in the US (Hale et al, 1962).…”

“…The field strain JF4278, isolated from mastitic milk in 2008 in Switzerland (Aebi et al, 2012), and the type strain PG45 (Hale et al, 1962) were used in this study. M. bovis cultures were grown in SP4 broth medium supplemented with 50 mg/ml cefoxitin sodium salt (Sigma-Aldrich, Buchs, Switzerland) for 18 h (Freundt, 1983).…”