Heroin-Induces Differential Protein Expression by Normal Human Astrocytes (NHA)

Reynolds, Jessica L.; Mahajan, Supriya D.; Sykes, Donald E.; Nair, Madhavan

doi:10.3844/ajidsp.2006.49.57

Cited by 21 publications

(17 citation statements)

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“…Creatine kinase B was downregulated by TH in our study, and by alcohol [54,56,60]. ATP synthase beta-chain with lower expression level was detected in a number of substances of abuse such as morphine [13,58], heroin [21], nicotine [52,53], and alcohol [55] by proteomic study. 14-3-3 protein epsilon was reduced by alcohol [23,60], Table 5 -Summary of proteins modified by tramadol and one or more other substances of abuse.…”

Section: Discussionmentioning

confidence: 58%

“…Energy metabolism Aldolase C Nicotine [52,53]; Alcohol [23,56]; Amphetamine [57]; Butorphanol [24]; Cocaine [17]; Heroin [21]; Morphine [11,58,62]; ATP synthase beta-chain Nicotine [52]; Amphetamine [57]; Alcohol [23,55]; Cocaine [59]; Heroin [21]; Morphine [11,13,14,58]; Creatine kinase B Alcohol [23,[54][55][56]60]; Methamphetamine [61]; Cocaine [17];…”

Section: Proteinmentioning

confidence: 99%

“…Recently, 2D-PAGE, in combination with mass spectrometry has been widely used in the identification of specific biochemical markers for diagnostic, preventive and therapeutic intervention in drug addiction, including morphine [11][12][13][14][15], cocaine [16][17][18], nicotine [19,20], heroin [21], alcohol [22,23] and butorphanol [24]. The neurotoxic effects of drug abuse are often associated with oxidative stress, mitochondrial dysfunction, apoptosis and inhibition of neurogenesis, among other mechanisms [25].…”

Section: Introductionmentioning

confidence: 99%

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Effects of chronic tramadol exposure on the zebrafish brain: A proteomic study

Zhuo

Huang

et al. 2012

Journal of Proteomics

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Section: Discussionmentioning

confidence: 58%

Section: Proteinmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of chronic tramadol exposure on the zebrafish brain: A proteomic study

Zhuo

Huang

et al. 2012

Journal of Proteomics

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“…We have shown that treatment of IDC and astrocytes with methamphetamine and heroin, respectively, increase the expression of galectin-1 (21, 39). Therefore, we sought to determine whether morphine could modulate galectin-1 gene expression in human MDM.…”

Section: Resultsmentioning

confidence: 99%

Morphine and Galectin-1 Modulate HIV-1 Infection of Human Monocyte-Derived Macrophages

Reynolds

Law

Mahajan

et al. 2012

The Journal of Immunology

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Morphine is a widely abused, addictive drug that modulates immune function. Macrophages are a primary reservoir of HIV-1; therefore, they play a role in the development of this disease, as well as impact the overall course of disease progression. Galectin-1 is a member of a family of β-galactoside–binding lectins that are soluble adhesion molecules and that mediate direct cell–pathogen interactions during HIV-1 viral adhesion. Because the drug abuse epidemic and the HIV-1 epidemic are closely interrelated, we propose that increased expression of galectin-1 induced by morphine may modulate HIV-1 infection of human monocyte-derived macrophages (MDMs). In this article, we show that galectin-1 gene and protein expression are potentiated by incubation with morphine. Confirming previous studies, morphine alone or galectin-1 alone enhance HIV-1 infection of MDMs. Concomitant incubation with exogenous galectin-1 and morphine potentiated HIV-1 infection of MDMs. We used a nanotechnology approach that uses gold nanorod–galectin-1 small interfering RNA complexes (nanoplexes) to inhibit gene expression for galectin-1. We found that nanoplexes silenced gene expression for galectin-1, and they reversed the effects of morphine on galectin-1 expression. Furthermore, the effects of morphine on HIV-1 infection were reduced in the presence of the nanoplex.

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“…The function of FAM3C is unknown, and it is unclear whether it is a secreted or a membrane protein. Tumor protein D54 has been identified by MS-based proteomics studies in normal human astrocytes (65), in breast tumor cells (66), and in lipid droplets from lipolytically stimulated (but not unstimulated) adipocytes (67). No studies of its physiological function have been reported.…”

mentioning

confidence: 99%

The Mycobacterium bovis Bacille Calmette-Guérin Phagosome Proteome

Lee

Jethwaney

Schilling

et al. 2010

Molecular & Cellular Proteomics

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Mycobacterium tuberculosis and Mycobacterium bovis bacille Calmette-Gué rin (BCG) alter the maturation of their phagosomes and reside within a compartment that resists acidification and fusion with lysosomes. To define the molecular composition of this compartment, we developed a novel method for obtaining highly purified phagosomes from BCG-infected human macrophages and analyzed the phagosomes by Western immunoblotting and mass spectrometry-based proteomics. Our purification procedure revealed that BCG grown on artificial medium becomes less dense after growth in macrophages. By Western immunoblotting, LAMP-2, Niemann-Pick protein C1, and syntaxin 3 were readily detectable on the BCG phagosome but at levels that were lower than on the latex bead phagosome; flotillin-1 and the vacuolar ATPase were barely detectable on the BCG phagosome but highly enriched on the latex bead phagosome. Immunofluorescence studies confirmed the scarcity of flotillin on BCG phagosomes and demonstrated an inverse correlation between bacterial metabolic activity and flotillin on M. tuberculosis phagosomes. By mass spectrometry, 447 human host proteins were identified on BCG phagosomes, and a partially overlapping set of 289 human proteins on latex bead phagosomes was identified. Interestingly, the majority of the proteins identified consistently on BCG phagosome preparations were also identified on latex bead phagosomes, indicating a high degree of overlap in protein composition of these two compartments. It is likely that many differences in protein composition are quantitative rather than qualitative in nature. Despite the remarkable overlap in protein composition, we consistently identified a number of proteins on the BCG phagosomes that were not identified in any of our latex bead phagosome preparations, including proteins involved in membrane trafficking and signal transduction, such as Ras GTPase-activating-like protein IQGAP1, and proteins of unknown function, such as FAM3C. Our phagosome purification procedure and initial proteomics analyses set the stage for a quantitative comparative analysis of mycobacterial and latex bead phagosome proteomes. Molecular & Cellular Proteomics 9:32-53, 2010.Mycobacterium tuberculosis, the etiological agent of tuberculosis, is a facultative intracellular bacterium. In human macrophages, M. tuberculosis resides in a membrane-bound phagosomal compartment that resists fusion with lysosomes and is only mildly acidified (1-5). In previous studies, using the cryosection immunogold technique, we have found that the M. tuberculosis phagosome exhibits delayed clearance of major histocompatability complex class I molecules and relatively weak staining for lysosomal membrane glycoproteins CD63, LAMP-1, 1 and LAMP-2 and the lysosomal acid protease cathepsin D (6 -10). Studies by other investigators have also demonstrated that M. tuberculosis and other mycobacterial species, including Mycobacterium bovis BCG, reside in phagosomes that resist acidification, are less mature, and less fusogenic with lysosomes...

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Heroin-Induces Differential Protein Expression by Normal Human Astrocytes (NHA)

Cited by 21 publications

References 59 publications

Effects of chronic tramadol exposure on the zebrafish brain: A proteomic study

Effects of chronic tramadol exposure on the zebrafish brain: A proteomic study

Morphine and Galectin-1 Modulate HIV-1 Infection of Human Monocyte-Derived Macrophages

The Mycobacterium bovis Bacille Calmette-Guérin Phagosome Proteome

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