Hesperidin attenuates benzo[α] pyrene-induced testicular toxicity in rats via regulation of oxidant/antioxidant balance

Abstract

Benzo[alpha]pyrene (BaP) is one of the polycyclic aromatic hydrocarbons, which has shown carcinogenic, teratogenic, and mutagenic potentials. The reproductive toxicity of BaP in male was not well investigated. Thereby, we have addressed in the current study the testicular toxicity of BaP and the postulate whether or not the citrus flavonoid, hesperidin (HDN), could ameliorate such toxicity in male Swiss albino rats. In this sense, animals were challenged with BaP (50 mg/kg/day, orally) for 10 consecutive days. HDN (200 mg/kg/day, orally) was administered ahead of BaP challenge for 10 consecutive days. BaP induced testicular toxicity that was well characterized histologically and biochemically. It decreased the relative testis weight and induced pyknosis and necrobiotic changes as well as chromatolysis in the nuclei of the spermatocytes in the seminiferous tubules. It also markedly deteriorated epididymal function as shown by decreased sperm count, motility, and daily sperm production. The polyaromatic hydrocarbon also reduced the testicular activities of lactate dehydrogenase (LDH-X), superoxide dismutase (SOD), and glutathione-S-transferase (GST). Besides, it decreased the testicular reduced glutathione (GSH) but increased malondialdehyde (MDA) contents. Prior administration of HDN ahead of BaP challenge ameliorated all the histological and biochemical alterations induced by BaP. It improved the epididymal function and mitigated the injurious effects of BaP on the seminiferous tubules. In conclusion, HDN has proven protective effects in BaP-induced testicular toxicity paradigm, and this protection resides, at least in part, on its antioxidant properties.

Combined Effects of Dibutyl Phthalate (DBP) and Benzo(a)Pyrene on Fertility in Male Rats

“…Other oxidative stress associated proteins like serotransferrin, ECE-2, retinoid binding protein, endoplasmin, hemopexin and ALDH2 were also associated with Bap and DBP exposure (Klipper et al, 2010). Suggesting that the exposure of Bap and DBP may disturb the oxidant/antioxidant balance and active the cAMP signal pathway in germ cells (Arafa et al, 2009;Mayati et al, 2012). Mitofilin, endoplasmin and MST4 are also associated with apoptotic process.…”

Comparison of toxicogenomics and traditional approaches to inform mode of action and points of departure in human health risk assessment of benzo[a]pyrene in drinking water

“…Doses as low as 1 mg/kg bw per day for 90-day exposures (Zheng et al, 2010) were effective in perturbing reproductive parameters. Major effects include impaired fertility in exposed adults (Arafa et al, 2009; Chen et al, 2011; Xu et al, 2010; Zheng et al, 2010) and developing rodents (Kristensen et al, 1995; Mackenzie and Angevine, 1981; Nakamura et al, 2012), which appears to be mediated by an AHR-dependent MOA (Neal et al, 2010; Sadeu and Foster, 2011). Overall, it is apparent that BaP affects the reproductive fitness of exposed organisms by impacting germ cell quality and quantity, hormone balance and gonadal tissue growth and development at exposure doses as low as 1 mg/kg bw per day in rodents (LOAEL).…”

Hesperidin Increase Cytotoxic Activity of Doxorubicin on Hela Cell Line Through Cell Cycle Modulation and Apoptotis Induction

“…Hesperidin also showed antiproliferative effect in MCF-7 cells transfected with green fluoresens protein (GFP)/alpha-tubulin (MCF-7-GFP-Tubulin) (Lee et al, 2009). It is also reported that hesperidin protective effect in benzo [a]pirene induced testicular toxicity paradigm and repaired the function of lactate dehydrogenase (LDH-X), superoxide dismutase (SOD), dan glutathione-Stransferase (GST) enzyme (Arafa et al, 2009). Previous study has reported that Hesperidin could induce apoptosis in human colon cancer cells through CASP3 activation.…”