Hetero-auxin als Stoffwechselprodukt niederer pflanzlicher Organismen. Isolierung aus Hefe. 13. Mitteilung über pflanzliche Wachstumsstoffe.

Kögl, Fritz; Kostermans, D. G. F. R.

doi:10.1515/bchm2.1934.228.3-6.113

Cited by 82 publications

(22 citation statements)

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“…These assays guided the isolation of IAA, including from plants (Kögl and others 1934;Kögl and Kostermans 1934;Berger and Avery 1944) and were indispensable for the success of such procedures. Similarly, these assays began the tissue specificity studies, allowing the concentrations in different tissues and at different times of development to be traced.…”

Section: Biological Assaysmentioning

confidence: 99%

“…It is 3-indole acetic acid and had been isolated previously from normal and pathological urines''. Kögl and Kostermans (1934) [Quote from Boysen-Jensen and others (1936)] '3-Indoleacetic acid has been isolated and identified as the auxin produced by alkaline hydrolysis of the purified precursor. This is the first independent confirmation of the presence of indoleacetic acid in higher plants' Berger and Avery (1944) Paper chromatography: ''The recent development of chromatographic techniques for the separation and identification of compounds has provided a new and valuable means for separation and identification of growth hormones, their precursors and breakdown products in plants' ' Yamaki (1950) [Quotation from Leopold (1967)] Isotope dilution analysis: ''Stable isotope dilution assays can be very accurate and precise because they correct for losses or inefficiencies in the sample preparation process as well as ion suppression effects during MS analysis'' Turian and Hamilton (1960) [Quotation from Novák and others (2014)] Isolation missteps: ''Evidence for the non-indolic nature of the new citrus auxin is presented on the basis of…thin-layer chromatography''…”

Section: Biological Assaysmentioning

confidence: 99%

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Analytical History of Auxin

Tivendale

Cohen

2015

J Plant Growth Regul

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Auxins are a group of naturally occurring phytohormones that have numerous functions in plant growth and development. The identification and quantification of these hormones from plant tissues has a long and varied history, dating back as far as the mid-19th century. The evolution of auxin analysis closely mirrors the evolution of analytical chemistry. However, despite the fact that the methods for auxin determination have evolved since the initial bioassays, few of the techniques developed over the years have been completely abandoned, and many of them are still used in routine auxin analyses today.

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Section: Biological Assaysmentioning

confidence: 99%

Section: Biological Assaysmentioning

confidence: 99%

Analytical History of Auxin

Tivendale

Cohen

2015

J Plant Growth Regul

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“…Although the chemical structure of the major natural auxin, indole-3-acetic acid (IAA), has been known for many years (2), the primary biochemical and molecular events responsible for auxin action are not yet understood. Among the first physiological changes observed after application of this phytohormone to plant tissues are an alteration of electrical potential of plasma membranes, an activation of H+-ATPase leading to H+ secretion, and a reorientation of cortical microtubules (3,4).…”

mentioning

confidence: 99%

An auxin-binding protein is localized to the plasma membrane of maize coleoptile cells: identification by photoaffinity labeling and purification of a 23-kda polypeptide.

Feldwisch

Zettl

Hesse

et al. 1992

Proc. Natl. Acad. Sci. U.S.A.

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Plasma membrane vesicles were isolated from maize (Zea mays L.) coleoptile tissue by aqueous twophase partitioning and assayed for homogeneity by the use of membrane-specific enzymatic assays. Using 5-azido- [7-3H]indole-3-acetic acid ([3H]N3IAA), we identified several IAAbinding proteins with molecular masses of 60 kDa (pm6O), 58 kDa (pm58), and 23 kDa (pm23). Using Triton X-114, we were able to selectively extract pm23 from the plasma membrane. We show that auxins and functional analogues compete with[3H]N3IAA for binding to pm23. We found that PAB130, a polyclonal antibody raised against auxin-binding protein 1 (ABP-1), recognized ABP-1 as well as pm23. This suggests that pm23 shares common epitopes with ABP-1. In addition, we identified an auxin-binding protein with a molecular mass of 24 kDa (pm24), which was detected in microsomal but not in plasma membrane vesicle preparations. Like pm23 this protein was extracted from membrane vesicles with Triton X-1 14. We designed a purification scheme allowing simultaneous purification of pm23 and pm24. Homogeneous pm23 and pm24 were obtained from coleoptile extracts after 7000-fold purification.

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“…It was apparent that some growth substance other than the above vitamins was present in "Marmite." It seemed possible that this could be IAA in view of the production of this substance by yeast cultures (Kogl and Kostermans 1934;Robinson and Stier 1941).…”

Section: ·7±1o·5mentioning

confidence: 99%

The Growth-Promoting Effect of Indole-3-Acetic Acid on the Common Cultivated Mushroom, Psalliota Hortensis (Cooke) Lange Forma Albida Lange

Fraser¹

1953

Aust. Jnl. Of Bio. Sci.

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SummaryA strain of the white-capped form of the common cultivated mushroom, Psalliota hortensis (Cook;e,} Lange forma albida Lange, appeared unable to grow appreciably on a liquid basal medium which contained glucose, asparagine, and mineral salts. Addition of thiamin and biotin increased the growth a little . . A number of other vitamins were without effect. Indole-3-acetic acid (IAA) in concentrations of from 5 to 100 mg/l markedly increased growth, particularly in the presence of thiamin and biotin, but only when the inoculum was floating on the surface of the culture medium. If the inoculum was submerged, addition of IAA had very little effect. The growth of both floating and submerged inocula was markedly increased by the addition of yeast extract, cotton wool extract, or mushroom compost extract, although these did not appear to contain appreciable quantities of IAA.The fungus grew fairly well on basal medium solidified with agar which had been purified to remove vitamins. Addition of thiamin or biotin did not promote growth. Concentrations of lAA of 10 and 100 mg/l almost completely prevented initial growth while concentrations of 0.001, 0.1, and 1.0 mg/l were without effect. However, after about 30 days vigorous growth commenced in the medium originally containing 10 mg/l IAA and proceeded at the same rate as in its absence. It seems probable that this was due to inactivation of the lAA by the small amount of mycelium formed.It is suggested that IAA may play a part in the growth processes of this fungus similar to that in higher plants, although the possibility that some related substance siIch as tryptophane is required for growth must also be considered, particularly in view of the unidentified growth-promoting factor present in various extracts.

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Hetero-auxin als Stoffwechselprodukt niederer pflanzlicher Organismen. Isolierung aus Hefe. 13. Mitteilung über pflanzliche Wachstumsstoffe.

Cited by 82 publications

References 0 publications

Analytical History of Auxin

Analytical History of Auxin

An auxin-binding protein is localized to the plasma membrane of maize coleoptile cells: identification by photoaffinity labeling and purification of a 23-kda polypeptide.

The Growth-Promoting Effect of Indole-3-Acetic Acid on the Common Cultivated Mushroom, Psalliota Hortensis (Cooke) Lange Forma Albida Lange

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