Heterogeneity in the expression of FcγRIII in morphologically mature granulocytes from patients with chronic myeloid leukemia

Four-Color Flow Cytometry Identifies Virtually All Cytogenetically Abnormal Bone Marrow Samples in the Workup of Non-CML Myeloproliferative Disorders

“…Previous studies typically have focused on one or a few myeloid antigens, including abnormal CD16 and CD56 in CML 4,5,8,9 or in both CML and NCMPDs 6 ; asynchronous CD14 and CD66 in a subset of myeloproliferative disorders 16 ; elevated platelet surface expression of P-selectin, thrombospondin, and c-Mpl in essential thrombocytosis 14,15 ; and increased expression of bcl-X L in polycythemia vera. 11 These studies generally did not use the 4-color technology that we used and generally did not have such a large series of cases.…”

“…By comparing patterns of antigen expression on a given cell population with the patterns identified on normal cells of that type, one potentially can identify abnormalities that, if sufficiently great, may substitute for clonality studies in identifying malignancy. A small number of studies during the past decade have reported abnormalities of one or a few myeloid antigens in CML, including decreased CD16, 4-6 decreased CD32, 5 decreased L-selectin expression on CD34+ cells, 7 aberrant expression of CD56 on blasts and myeloid cells, 8,9 and aberrant expression of lymphoid antigens such as CD2, CD5, and CD7 on the blasts in CML blast crisis. 10 Reported flow cytometric abnormalities among the NCMPDs have included the Particularly useful combinations of antibodies were those that identified antigens expressed over a wide range of intensities in the population of interest.…”