Heterogeneity of acid secretion induced by carbachol and histamine along the gastric gland axis and its relationship to [Ca<sup>2+</sup>]<sub>i</sub>

Perez‐Zoghbi, Jose F.; Mayora, Adriana; Ruiz, Marie Christine; Michelangeli, Fabián A.

doi:10.1152/ajpgi.90224.2008

Cited by 13 publications

(5 citation statements)

References 52 publications

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“…Pepsin inactivation occurs at about pH 6. Between pH 4 and 6, pepsin is still stable but inactive [45]. Gastric pH values obtained in all the experiments in response to extract administration ranged between 3.97 and 5.26, suggesting that the extract may deactivate gastric pepsin and interfere with protein digestion.…”

Section: Discussionmentioning

confidence: 99%

Antisecretory Action of the Extract of the Aerial Parts ofEremomastax speciosa (Acanthaceae)Occurs through Antihistaminic and Anticholinergic Pathways

Perfusion

Tan

Ernestine

et al. 2014

Advances in Pharmacological Sciences

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Objective. The objective of this study was to find out the possible antiulcer mechanism of action of Eremomastax speciosa. Method. Carbachol- and histamine-induced hypersecretion, associated with the pylorus ligation technique, were used in rats. Gastric mucosal ulceration, mucus production, pH, gastric volume, and acidity were measured. Results. Histamine and carbachol raised gastric acidity to 86.50 and 84.80 mEq/L, respectively, in the control rats, and the extracts (200 mg/kg) reduced gastric acidity to 34.60 and 39.00 mEq/L, respectively. Intraduodenal aqueous extract (400 mg/kg) in histamine- and carbachol-treated rats produced significant (P < 0.001) decreases in acid secretion to 28.50 and 28.80 mEq/L, respectively, and 100 percent inhibition of gastric ulceration. Augmented histamine-induced gastric acid secretion (90.20 mEq/L) was significantly reduced to 52.60 and 27.50 mEq/L by the 200 and 400 mg/kg doses of the aqueous extract, respectively. The extract significantly reduced (P < 0.001) the volume of gastric secretion and significantly increased mucus production. The ulcer inhibition potential of the extract significantly dropped to 25–44% (oral extract) and to 29–37% (duodenal extract) in carbachol/indomethacin-treated rats. Conclusion. The aqueous extract of E. speciosa has both cytoprotective and antisecretory effects. The antisecretory effect may involve a mechanism common to both cholinergic and histaminergic pathways.

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Section: Discussionmentioning

confidence: 99%

Antisecretory Action of the Extract of the Aerial Parts ofEremomastax speciosa (Acanthaceae)Occurs through Antihistaminic and Anticholinergic Pathways

Perfusion

Tan

Ernestine

et al. 2014

Advances in Pharmacological Sciences

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“…This potential relationship between Zn and Ca 2+ levels may in part explain the role of Zn and Zn transporters in the intestinal secretion (Liu et al 2011). Based on the well-recognized role of Ca 2+ as a second messenger in responses to secretagogues (Caroppo et al 2001; Perez-Zoghbi et al 2008), there is a novel concept that Ca 2+ may be also a second messenger that can match the basolateral demand for Zn with the secretory response to physiologic stimulation (Liu et al 2011). Similar connections may be also present in other secretory cells including neural, endocrine, and exocrine tissues.…”

Section: Discussionmentioning

confidence: 99%

Neurochemical Characterization of Zinc Transporter 3-Like Immunoreactive (ZnT3+) Neurons in the Intramural Ganglia of the Porcine Duodenum

et al. 2012

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The SLC30 family of divalent cation transporters is thought to be involved in the transport of zinc in a variety of cellular pathways. Zinc transporter 3 (ZnT3) is involved in the transport of zinc into synaptic vesicles or intracellular organelles. As the presence of ZnT3 immunoreactive neurons has recently been reported in both the central and peripheral nervous systems of the rat, the present study was aimed at disclosing the presence of a zinc-enriched neuron enteric population in the porcine duodenum to establish a preliminary insight into their neurochemical coding. Double- and triple-immunofluorescence labeling of the porcine duodenum for ZnT3 with the pan-neuronal marker (PGP 9.5), substance P, somatostatin, vasoactive intestinal peptide (VIP), nitric oxide synthase (NOS), leu-enkephalin, vesicular acetylcholine transporter (VAChT), neuropeptide Y, galanin (GAL), and calcitonin gene-related peptide were performed. Immunohistochemistry revealed that approximately 35, 43, and 48 % of all PGP9.5-postive neurons in the myenteric (MP), outer submucous (OSP), and inner submucous (ISP) plexuses, respectively, of the porcine duodenum were simultaneously ZnT3+. In the present study, ZnT3+ neurons coexpressed a broad spectrum of active substances, but co-localization patterns unique to the plexus were studied. In the ISP, all ZnT3+ neurons were VAChT positive, and the largest populations among these cells formed ZnT3+/VAChT+/GAL+ and ZnT3+/VAChT+/VIP+ cells. In the OSP and MP, the numbers of ZnT3+/VAChT+ neurons were two times smaller, and substantial subpopulations of ZnT3+ neurons in both these plexuses formed ZnT3+/NOS+ cells. The large population of ZnT3+ neurons in the porcine duodenum and a broad spectrum of active substances which co-localize with this peptide suggest that ZnT3 takes part in the regulation of various processes in the gut both in normal physiology and during pathological processes.

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“…M 3 R is stimulated by cholinergics and produces inositol 1,4,5-trisphosphatase (IP 3 ) in the cytosol, which can trigger Ca 2+ release from the endoplasmic reticulum (ER) so that the intracellular Ca 2+ concentration increases. The increase in the intracellular Ca 2+ concentration can promote H 2 O e ux, ion e ux, and ion in ux [35][36][37] .…”

Section: Discussionmentioning

confidence: 99%

Cortex Lycii attenuates night sweat in Yin-deficient rat by downregulating β 2 AR/cAMP signaling: A proteomic approach

Huang,

Hong,

Wang

et al. 2024

Preprint

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Purpose: To investigate the therapeutic effect of Cortex Lycii on the sweat secretion of YDH rats and its underlying mechanism. Methods: Male SD rats (N=36) were randomly assigned into Control, YDH, and DI groups (N=12 each group). YDH model rats were constructed by oral administration of thyroxin and reserpine. After two-weeks treatment of Cortex Lycii, the pharmacodynamics was assessed then the protein profile was obtained using a tandem mass tag proteomic approach. Gene ontology and Kyoto Encyclopedia of Genes and Genomes analysis was used for bioinformatics analysis of differential expressed proteins (DEPs), critical DEPs were validated by Western blot. The receptor involved in the Cortex Lycii were confirmed by using β2AR receptor agonist (isoproterenol)- and M3R receptor agonist (pilocarpine)-induced sweat secretion in YDH rats. Results: Cortex Lycii attenuates sweat secretion in YDH rats, and down-regulated the expression of AQP5 and β2-receptor on paw pads of YDH rats. Proteomics analysis revealed 189 differential proteins between the model and control groups, of which 124 differential proteins were remarkably reversed by Cortex Lycii. The overlapping DEPs in the model and Cortex Lycii groups were mainly enriched in the β2AR/cAMP pathway. Moreover, Cortex Lycii considerably attenuated the agonistic effect of isoprenaline induced sweat secretion. Conclusion: This study demonstrated the effect of Cortex Lycii on sweat secretion in Yin-deficient rats. The mechanism on the ground may refer to the inhibition of β2AR/cAMP, followed by the suppression of the downstream PKA/ezrin/CFTR signaling pathway to reduce ion exchange and the inhibition of IP3/Ca2+/AQP5. The finding may provide new research on the mechanism of night sweats in TCM and their amelioration by Cortex Lycii.

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Heterogeneity of acid secretion induced by carbachol and histamine along the gastric gland axis and its relationship to [Ca²⁺]_i

Abstract: Perez-Zoghbi JF, Mayora A, Ruiz MC, Michelangeli F. Heterogeneity of acid secretion induced by carbachol and histamine along the gastric gland axis and its relationship to [Ca 2ϩ ]i.

Cited by 13 publications

References 52 publications

Antisecretory Action of the Extract of the Aerial Parts ofEremomastax speciosa (Acanthaceae)Occurs through Antihistaminic and Anticholinergic Pathways

Antisecretory Action of the Extract of the Aerial Parts ofEremomastax speciosa (Acanthaceae)Occurs through Antihistaminic and Anticholinergic Pathways

Neurochemical Characterization of Zinc Transporter 3-Like Immunoreactive (ZnT3+) Neurons in the Intramural Ganglia of the Porcine Duodenum

Cortex Lycii attenuates night sweat in Yin-deficient rat by downregulating β 2 AR/cAMP signaling: A proteomic approach

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Heterogeneity of acid secretion induced by carbachol and histamine along the gastric gland axis and its relationship to [Ca2+]i

Abstract: Perez-Zoghbi JF, Mayora A, Ruiz MC, Michelangeli F. Heterogeneity of acid secretion induced by carbachol and histamine along the gastric gland axis and its relationship to [Ca 2ϩ ]i.

Cited by 13 publications

References 52 publications

Antisecretory Action of the Extract of the Aerial Parts ofEremomastax speciosa (Acanthaceae)Occurs through Antihistaminic and Anticholinergic Pathways

Antisecretory Action of the Extract of the Aerial Parts ofEremomastax speciosa (Acanthaceae)Occurs through Antihistaminic and Anticholinergic Pathways

Neurochemical Characterization of Zinc Transporter 3-Like Immunoreactive (ZnT3+) Neurons in the Intramural Ganglia of the Porcine Duodenum

Cortex Lycii attenuates night sweat in Yin-deficient rat by downregulating β 2 AR/cAMP signaling: A proteomic approach

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Heterogeneity of acid secretion induced by carbachol and histamine along the gastric gland axis and its relationship to [Ca²⁺]_i