Heterogeneity of amino acid transport activity in the avian erythrocyte

Lerner, Joseph; Messier, Denise L; Neuman, Robert G; Sattelmeyer, Peggy A.; Estes, Jane S.P.; Smagula, Rosalita M; Somes, Ralph G.

doi:10.1016/0300-9629(78)90248-7

Cited by 10 publications

(2 citation statements)

References 6 publications

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“…It can be concluded without ambiguity that the differences in the rates are not due to different intracellular amino acid concentrations. Heterogeneity of amino-acid transport in chicken erythrocytes was first described by Lerner et al (1978) and Somes et al (1981) who demonstrated that there were important individual differences in the ability to transport leucine and lysine and, furthermore, that these differences could be emphasized by genetic selection. The ratio of the magnitude of leucine influx (100 M) in the high and low transport lines, for the first, second and third generations of chickens, was 1.58, 4.88, and 7.08, respectively.…”

Section: Discussionmentioning

confidence: 98%

“…Important individual differences in the capacity to transport amino acids were noticed in early studies with chicken erythrocytes (Lerner et al, 1978). To investigate the origin of the heterogeneity, animals were selected for either low or high transport rates and the amino-acid transport properties of the progeny were analyzed in three consecutive generations (Somes, Smagula & Lerner, 1981).…”

Section: Introductionmentioning

confidence: 99%

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System y + L-like Activities Account for High and Low Amino-Acid Transport Phenotypes in Chicken Erythrocytes

Vargas

Devés

2001

Journal of Membrane Biology

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The functional properties of the transport of lysine across the chicken erythrocyte membrane were investigated. The animal population studied (male Leghorn chickens, 6-14 weeks old) was found to consist of two groups presenting either low (LT, 19 individuals) or high transport rates (HT, 20 individuals). The rates of influx in the two groups, measured at a concentration of l-lysine of 1 microm, differed by a factor of 34. The transport activities observed in LT and HT erythrocytes were compatible with the general features of system y+L, but showed some differences in specificity. The transporter in the LT group was found to bind l-lysine, l-leucine, l-methionine and l-glutamine with high affinity, in the presence of sodium, as described for system y+L in human erythrocytes. The activity present in HT erythrocytes exhibited a much lower affinity for l-leucine, but was able to interact strongly with l-glutamine and l-methionine. The specificity pattern of the HT transporter, has not been described in other cell types. In other respects, the properties of the two systems were similar. Sodium replacement with potassium, drastically reduced the affinity for l-leucine, without affecting lysine transport. Both transporters function as tightly coupled exchangers, are inactivated by p-chloromercuribenzene sulfonate and resistant to N-ethylmaleimide. These findings explain previous results obtained in selective breeding experiments of chicken with high and low amino-acid transport activity.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

System y + L-like Activities Account for High and Low Amino-Acid Transport Phenotypes in Chicken Erythrocytes

Vargas

Devés

2001

Journal of Membrane Biology

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Testing the Hypothesis that System y+L Accounts for High- and Low-Transport Phenotypes in Chicken Erythrocytes Using L-Leucine as Substrate

et al. 2005

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Experiments were carried out to test the hypothesis that system y(+)L accounts for the high (HT) and low (LT) amino-acid transport phenotypes in chicken erythrocytes and to explain the different effect of selective breeding on lysine and leucine fluxes. L: -Leucine transport was characterized in individuals which had been separated into two groups (HT and LT) according to their capacity to transport L: -lysine across the erythrocyte membrane. Whereas lysine influx (1 muM: ) in the two groups differed by 32-fold (HT/LT), leucine influx was not significantly different. Average rates (nmol/ L cells/ min) were: 227 (HT) and 7.0 (LT) for L: -lysine, and 8.9 (HT) and 7.1 (LT) for L: -leucine. The differential ability of L: -lysine and L: -leucine fluxes to discriminate between the HT and LT phenotypes was shown to be consistent with the interactions of these substrates with system y(+)L and to vary depending on the conditions of the assay. It is shown that the two phenotypes can be clearly discriminated by measuring L: -leucine influx in the presence of Li(+). These results support the hypothesis that the HT and LT phenotypes reflect alterations in the function of system y(+)L and illustrate that the choice of the appropriate substrate and medium composition must be carefully considered when investigating the consequences of either experimental or natural alterations of broad-scope transporters.

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Myo-inositol transport in the small intestine of the domestic fowl

Lerner

Smagula

1979

Comparative Biochemistry and Physiology Part A: Physiology

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Heterogeneity of amino acid transport activity in the avian erythrocyte

Cited by 10 publications

References 6 publications

System y + L-like Activities Account for High and Low Amino-Acid Transport Phenotypes in Chicken Erythrocytes

System y + L-like Activities Account for High and Low Amino-Acid Transport Phenotypes in Chicken Erythrocytes

Testing the Hypothesis that System y+L Accounts for High- and Low-Transport Phenotypes in Chicken Erythrocytes Using L-Leucine as Substrate

Myo-inositol transport in the small intestine of the domestic fowl

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