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A 1-MHz continuous wave sound field of intensity 0.6 W/cm2 was applied to a suspension of avian erythrocytes in vitro for 30 min. Total uptake (diffusion plus nonspecific binding plus mediated transport) was determined by incubation of 1-ml aliquots of insonated and control cells for 10 min in labeled leucine. The percentage of the total uptake due to mediated transport was determined by a similar incubation in a 50:1 mixture of the competitive inhibitor isoleucine and labeled leucine. At the intensities used the sonated cells absorbed approximately 5% less labeled leucine than the control cells. Analysis shows the effect to be statistically significant (P < 0.01). However, results for the effect of ultrasound on mediated transport are not statistically significant (P > 0.9). Therefore, the greatest effect of ultrasound using these experimental conditions is on the diffusion component of transport. [This work was supported in part by the American Heart Association-Maine Affiliate, NIH via AM 19445 and the Maine State Experiment Station.]

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