Heterogeneity of Erythrocyte Catalase II

Aebi, H.; Wyss, Sonja R.; B, Scherz; Skvaril, F

doi:10.1111/j.1432-1033.1974.tb03751.x

Cited by 234 publications

(114 citation statements)

References 27 publications

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“…The activity of 256 Cold Acclimation on Antioxidant Status Values are mean and SE SOD was measured according to the method of Marklund and Marklund (1974). The catalase (CAT) activity was measured by the method of Abei et al (1974) in which the H 2 O 2 concentration was obtained by the molar absorptivity of H 2 O 2 using the changes in the absorption of H 2 O 2 at 240 nm for 5 min. The hemolysate was diluted 5 times and used as the raw material for the GSHpx activity measurement.…”

Section: Measurement Of Antioxidative Enzyme Activity and Gsh Level Imentioning

confidence: 99%

Effect of Cold Acclimation on Antioxidant Status in Cold Acclimated Skaters

Hong

Kim

Lee

2008

J Physiol Anthropol

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We investigated whether cold acclimation leads to increased activity of the antioxidant defense enzymes and muscle injury. Comparisons were between short track skaters (nϭ6) and inline skaters (nϭ6) during rest and at submaximal cycling (65% VO 2 max) in cold (ambient temperature: 5Ϯ1°C, relative humidity: 41Ϯ8%) and warm conditions (ambient temperature: 21Ϯ1°C, relative humidity: 35Ϯ5%), during 60 min, respectively, and during the recovery phase. Erythrocyte superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHpx), reduced glutathione (GSH), thiobarbituric substance acid (TBARS), serum creatine kinase (CK), lactate dehydrogenase (LDH), plasma myoglobin (Mb) and cortisol were determined. Activities of CAT and GSHpx and the level of GSH and TBARS in erythrocyte and the level of LDH in serum were elevated in cold acclimated subjects. We suggested that the compensatory increase in antioxidative defense enzymes resulting from long-term cold exposure may reflect the elevated reactive oxygen species (ROS) production and muscle injury at this environment acclimation.

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Section: Measurement Of Antioxidative Enzyme Activity and Gsh Level Imentioning

confidence: 99%

Effect of Cold Acclimation on Antioxidant Status in Cold Acclimated Skaters

Hong

Kim

Lee

2008

J Physiol Anthropol

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“…Interestingly, an unstable catalase isolated from patients homozygous for a Swiss-type acatalasemia, a hereditary catalase deficiency disorder, rapidly disassociates into inactive dimers with reduced heme content. This suggests that catalase assembly variants may play roles in disease susceptibility (Aebi et al, 1974), in addition to nonsense and splicing mutations (Hirono et al, 1995). From the structural data, a mechanism for the recognition and removal of peroxide by catalase has been proposed (Putnam et al, 2000).…”

Section: Hydrogen Peroxide Removal By Catalasementioning

confidence: 99%

Developing master keys to brain pathology, cancer and aging from the structural biology of proteins controlling reactive oxygen species and DNA repair

2007

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This review is focused on proteins with key roles in pathways controlling either reactive oxygen species or DNA damage responses, both of which are essential for preserving the nervous system. An imbalance of reactive oxygen species or inappropriate DNA damage response likely causes mutational or cytotoxic outcomes, which may lead to cancer and/or aging phenotypes. Moreover, individuals with hereditary disorders in proteins of these cellular pathways have significant neurological abnormalities. Mutations in a superoxide dismutase, which removes oxygen free radicals, may cause the neurodegenerative disease amyotrophic lateral sclerosis. Additionally, DNA repair disorders that affect the brain to varying extents include ataxia-telangiectasia-like disorder, Cockayne syndrome or Werner syndrome. Here, we highlight recent advances gained through structural biochemistry studies on enzymes linked to these disorders and other related enzymes acting within the same cellular pathways. We describe the current understanding of how these vital proteins coordinate chemical steps and integrate cellular signaling and response events. Significantly, these structural studies may provide a set of master keys to developing a unified understanding of the survival mechanisms utilized after insults by reactive oxygen species and genotoxic agents, and also provide a basis for developing an informed intervention in brain tumor and neurodegenerative disease progression.

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“…One unit of (U) CAT was defined as the amount of enzyme required to decompose 1 lmol of H 2 O 2 per min, at 25°C and pH 7.0. Results are expressed as units (U) of CAT activity/g wet tissue [12].…”

Section: Catalase Activity (Cat)mentioning

confidence: 99%

Role of Measurement of Antioxidant Enzymes in Evaluation of Antioxidant Therapy in Tobacco Abusers with Oral Leukoplakia

Jain

Singh

et al. 2011

Indian J Otolaryngol Head Neck Surg

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Antioxidants are widely used in chemoprevention of malignancy. Numerous studies in medical literature have reported the evaluation of this treatment protocol by indirect methodology-epidemiology, invitro studies, pharmacology and animal models etc. However, there is a paucity of literature on the measurement of antioxidant enzymes as a parameter for assessing the outcome of antioxidant therapy. This study explores the efficacy and outcome of antioxidant enzyme assay in relation to antioxidant therapy in tobacco abusers, hitherto unreported in medical literature. A prospective cohort study with control in 50 patients carried out at a tertiary care teaching Institution (Institute of Medical Sciences, Banaras Hindu University, Varanasi, India). Out of these patients, 10 patients acted as control, rest 40 patients-all tobacco users in some form, were divided into three groups on the basis of histopathological grading of dysplasia-no dysplasia, mild or moderate dysplasia. The levels of Lipid peroxidase (LPO), Superoxide dismutase (SOD) and Catalase (CAT) in mucosa and serum were assayed in each group, and re-evaluated at the end of 3 months after intervention with antioxidant treatment. To detect any alteration in degree of dysplasia a repeat biopsy was also done at the end of 3 months. The results were statistically analysed using paired t test. A statistically significant decrease in level of LPO and SOD, and an increase in CAT levels were recorded both in mucosa and serum. However, no change in dysplasia and no new case of dysplasia were observed. Further, antioxidant treatment was continued for a year and the final out come of the lesion was assessed by ''Carter's criteria''. A final success rate of 74.19% was recorded in terms of partial or complete regression of the lesion. This study confirms the therapeutic efficacy of antioxidants in oral leukoplakia, and cites the importance of LPO, SOD and CAT in evaluating the efficacy of antioxidant treatment. However, the study failed to elucidate any relationship between enzyme measurement and the final outcome of the lesion.

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Heterogeneity of Erythrocyte Catalase II

Cited by 234 publications

References 27 publications

Effect of Cold Acclimation on Antioxidant Status in Cold Acclimated Skaters

Effect of Cold Acclimation on Antioxidant Status in Cold Acclimated Skaters

Developing master keys to brain pathology, cancer and aging from the structural biology of proteins controlling reactive oxygen species and DNA repair

Role of Measurement of Antioxidant Enzymes in Evaluation of Antioxidant Therapy in Tobacco Abusers with Oral Leukoplakia

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