Heterogeneity of in vitro radiosensitivity in human bladder cancer cells

Barnetson, Anthony R.; Banasiak, Dorota; Fisher, Richard; Mameghan, Hedy; Ribeiro, Julio; Brown, Keith; Brown, Joanne; O’Mara, Shane M.; Russell, Pamela J.

doi:10.1002/(sici)1520-6823(1999)7:2<66::aid-roi2>3.0.co;2-t

Cited by 12 publications

(7 citation statements)

References 43 publications

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“…The SF 2 for our cell lines ranged from 0.56 to 0.63, with a mean of 0.61. Our results are similar to reported SF 2 of human bladder cancer cell lines ranging from 0.44 to 0.87 22,23 . The moderately high SF identified in our cell lines is consistent with a relatively radioresistant tissue.…”

Section: Discussionsupporting

confidence: 91%

Radiosensitivity and capacity for radiation‐induced sublethal damage repair of canine transitional cell carcinoma (TCC) cell lines

Parfitt

Milner

Salute

et al. 2011

Vet Comparative Oncology

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Understanding the inherent radiosensitivity and repair capacity of canine transitional cell carcinoma (TCC) can aid in optimizing radiation protocols to treat this disease. The objective of this study was to evaluate the parameters surviving fraction at 2 Gy (SF(2) ), α/β ratio and capacity for sublethal damage repair (SLDR) in response to radiation. Dose-response and split-dose studies were performed using the clonogenic assay. The mean SF(2) for three established TCC cell lines was high at 0.61. All the three cell lines exhibited a low to moderate α/β ratio, with the mean being 3.27. Two cell lines exhibited statistically increased survival at 4 and 24 h in the dose-response assay. Overall, our results indicate that the cell lines are moderately radioresistant, have a high repair capacity and behave similarly to a late-responding normal tissue. These findings indicate that the radiation protocols utilizing higher doses with less fractionation may be more effective for treating TCC.

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Section: Discussionsupporting

confidence: 91%

Radiosensitivity and capacity for radiation‐induced sublethal damage repair of canine transitional cell carcinoma (TCC) cell lines

Parfitt

Milner

Salute

et al. 2011

Vet Comparative Oncology

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“…PRO/REG sublines were obtained from the same parental rat colon tumour, which made possible molecular and cellular investigations on HRS response in the frame of tumour heterogeneity. Here, our data indicate that PRO cells show more HRS response than REG cells, consistently with literature showing that tumorigenic cells are generally more radiosensitive than non-tumorigenic cells (e.g., Barnetson et al 1999). Our observations provide evidence that neither cell cycle distribution alone (both sublines elicited similar S-G 2 /M fraction before irradiation) nor p53-dependent apoptosis (not relevant here) nor HDC (REG cells spontaneously showed more HDC than PRO cells) may explain the fact that PRO cells show higher HRS response than REG cells.…”

Section: Pro/reg Sublines: a Useful Model To Study Hrs Responsesupporting

confidence: 91%

Low-dose hyper-radiosensitivity of progressive and regressive cells isolated from a rat colon tumour: Impact of DNA repair

Thomas

Charrier

Massart

et al. 2008

International Journal of Radiation Biology

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HRS response of PRO subline may be induced by impairments in NHEJ repair that targets G(1) cells and RAD51-dependent repair that targets S-G(2)/M cells. The cellular consequences of such impairments are a failure to arrest in cell cycle, the propagation of damage through cell cycle, mitotic death but not p53-dependent apoptosis. Tumourigenic cells with high metastatic potential may preferentially show HRS response.

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“…Clonogenic assays were performed as described [32]. Preliminary experiments were performed on each cell line to determine its optimal plating density to form colonies.…”

Section: Clonogenic Assaymentioning

confidence: 99%

“…Cell survival curves were fitted using a linearquadratic (LQ) model [32], ln[PE] ¼ m À ad À bd 2 where PE is the mean plating efficiency at dose dGy. The radiosensitivity parameters a and b are constants.…”

Section: Clonogenic Assaymentioning

confidence: 99%

Paclitaxel enhanced radiation sensitization for the suppression of human prostate cancer tumor growth via a p53 independent pathway

et al. 2007

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Heterogeneity of in vitro radiosensitivity in human bladder cancer cells

Cited by 12 publications

References 43 publications

Radiosensitivity and capacity for radiation‐induced sublethal damage repair of canine transitional cell carcinoma (TCC) cell lines

Radiosensitivity and capacity for radiation‐induced sublethal damage repair of canine transitional cell carcinoma (TCC) cell lines

Low-dose hyper-radiosensitivity of progressive and regressive cells isolated from a rat colon tumour: Impact of DNA repair

Paclitaxel enhanced radiation sensitization for the suppression of human prostate cancer tumor growth via a p53 independent pathway

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