Heterogeneity of Rabbit Serum Interferons

The experiments reported in the preceding paper (1) revealed essentially similar kinetics of interferon synthesis in primary cultures of rabbit macrophages and renal epithelium. Although interferon yields from uninfected macrophages were much lower than those from virus-infected cells, rates of synthesis were similar and actinomycin introduced soon after induction inhibited interferon production in all in vitro systems. The only significant difference in interferon-synthesizing capacity of virus-infected macrophages and kidney cells was the time after induction at which the respective cell types became refractory to the inhibitory action of actinomycin. None of these data can be interpreted as indicating that more than one molecular species of interferon is synthesized by different rabbit cells in response to the same or different inducing agents. However, other investigators have found that the stability and molecular weights of mouse, rabbit, and human interferons appear to depend on the producing cell type and the viral or nonviral nature of the inducing agents (2-4). xThe present report presents evidence that macrophages and other ceils can produce several molecular species of interferon that are heterogeneous with respect to molecular weight despite their apparent similarity in biological and other physicochemical properties. Materials and MethodsSources of Interferons.--Detdals of the procedures were presented in the preceding report (1). Virus-induced interferons were prepared by harvesting media from primary cultures of 2-8 X l0 T rabbit peritoneal macrophages or 2--4 X 106 rabbit kidney (RK) l cells that had

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“…These data are consistent with the finding of Ke et al (9) that sera of NDVinfected rabbits contain at least two molecular species of interferon.…”

Section: Sephadex Gel Filtrationsupporting

confidence: 93%

Rabbit Macrophage Interferons

Smith

Wagner

1967

The Journal of Experimental Medicine

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“…RM cultures were usually seeded with [2][3][4][5][6][7][8] x lo7 macrophages in 5 ml RM growth medium. The details of the procedure were previously reported (9).…”

mentioning

confidence: 99%

Interferon Induced by Endotoxin and Newcastle Disease Virus in Rabbit Macrophage and Kidney Cell Cultures

Smith

Lubiniecki

Armstrong

et al. 1973

Experimental Biology and Medicine

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“…~bereinstim-mend wurde eine I-IM-Komponente mit einem Molgewicht yon 100.000 (oder mehr) beschrieben (4,9,11). Gegens~tzliche Angaben dagegen wurden fiber das Molgewicht der NM-Aktivit/~t mit 46.000 (4, 9) bzw.…”

Section: Diskussionunclassified

“…Auf Grund der Elution der l~eferenzsubstanzen, die ebenfalls in Harnstoff-Milieu ehromatographiert worden waren, wurde das Molgewicht des einen Gipfels mit 50--55.000 sowie das des anderen Gipfels Init 40--43.000 geschgtzt. Diese Werte entsprechen etwa den Angaben anderer Autoren mit MG 52.000 (11) und 46.000 (4,9).…”

Section: Diskussionunclassified

Physikalisch-chemische Eigenschaften Myxovirus-induzierter Seruminterferone des Kaninchens

Kadri

Kohlhage

1969

Archiv f Virusforschung

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Heterogeneity of Rabbit Serum Interferons

Cited by 32 publications

References 7 publications

Rabbit Macrophage Interferons

Rabbit Macrophage Interferons

Interferon Induced by Endotoxin and Newcastle Disease Virus in Rabbit Macrophage and Kidney Cell Cultures

Physikalisch-chemische Eigenschaften Myxovirus-induzierter Seruminterferone des Kaninchens

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