Heterogeneous and Flexible Transmission of
            <i>mcr-1</i>
            in Hospital-Associated Escherichia coli

Shen, Yingbo; Wu, Zhongliang; Wang, Yan; Zhang, Rong; Zhou, Hongwei; Wang, Shaolin; Lei, Lei; Mei, Li; Cai, Jiachang; Tyrrell, Jonathan M.; Tian, Guo‐Bao; Wu, Congming; Zhang, Qijing; Shen, Jianzhong; Walsh, Timothy R.; Shen, Zhangqi

doi:10.1128/mbio.00943-18

Cited by 57 publications

(34 citation statements)

References 69 publications

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“…In addition, the mcr-1 positive isolates showed higher resistance to PB than the mcr-1 negative E. coli isolates in this study, which suggested that there was a close positive correlation between the resistance phenotypes and genotypes of the mcr-1 positive isolates. This result is consistent with other reports (Huang et al, 2017;Shen et al, 2018;Zhuge et al, 2019).…”

Section: Discussionsupporting

confidence: 94%

Prevalence and Molecular Characteristics of Avian-Origin mcr-1-Harboring Escherichia coli in Shandong Province, China

et al. 2020

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The objective of this study was to evaluate the prevalence and characteristics of avian-origin mcr-1-harbouring Escherichia coli in Shandong Province, China. During 2017-2018, a total of 668 non-duplicate E. coli isolates were separately collected from 8eight large intensive poultry farms in Shandong Province. Antimicrobial susceptibility testing for 10 antimicrobial agents commonly used in farms was performed on all E. coli isolates by the agar dilution method; the mobile colistin resistance gene (mcr-1) gene was screened by PCR, and mcr-1 positive isolates were PCR-screened for antimicrobial resistance genes and typed by multi-locus sequence typing (MLST). Among the 668 E. coli, 102 (15.3%) harbored the mcr-1 gene; high antimicrobial resistance rates were observed for ampicillin (100/102, 98.0%), followed by amoxicillin (99/102, 97.1%) and florfenicol (97/102, 95.1%), and a low level of resistance was found for amoxicillin/clavulanic acid (24/102, 23.5%). Five ESBL genes were detected, and all isolates carried bla TEM (102/102, 100%), followed by bla CTX−M (90/102, 88.2%). Four PMQR genes were detected; aac(6)-Ib-cr (40/102, 39.2%) was the most commonly isolated PMQR gene, followed by qnrA (10/102, 9.8%). Thirty-eight different kinds of STs were identified, and the dominant ST was ST93 (19/102, 18.6%), followed by ST48 (9/102, 8.8%). In summary, E. coli from poultry in Shandong could be a reservoir for the mcr-1 gene, which could pose serious risks to human public health.

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Section: Discussionsupporting

confidence: 94%

Prevalence and Molecular Characteristics of Avian-Origin mcr-1-Harboring Escherichia coli in Shandong Province, China

et al. 2020

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“…This is consistent with a recent report that the insertion of IS Kpn26 to the upstream of mcr-1 in E . coli isolates ZJ148 and ZJ1653 did not affect the colistin resistance ( Shen et al, 2018 ). We then reasoned that the host strain 14EC047 might suppress the activity of MCR-1.…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial Resistance Profile of mcr-1 Positive Clinical Isolates of Escherichia coli in China From 2013 to 2016

Zhao

et al. 2018

Front. Microbiol.

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Multidrug-resistant (MDR) Escherichia coli poses a great challenge for public health in recent decades. Polymyxins have been reconsidered as a valuable therapeutic option for the treatment of infections caused by MDR E. coli. A plasmid-encoded colistin resistance gene mcr-1 encoding phosphoethanolamine transferase has been recently described in Enterobacteriaceae. In this study, a total of 123 E. coli isolates obtained from patients with diarrheal diseases in China were used for the genetic analysis of colistin resistance in clinical isolates. Antimicrobial resistance profile of polymyxin B (PB) and 11 commonly used antimicrobial agents were determined. Among the 123 E. coli isolates, 9 isolates (7.3%) were resistant to PB and PCR screening showed that seven (5.7%) isolates carried the mcr-1 gene. A hybrid sequencing analysis using single-molecule, real-time (SMRT) sequencing and Illumina sequencing was then performed to resolve the genomes of the seven mcr-1 positive isolates. These seven isolates harbored multiple plasmids and are MDR, with six isolates carrying one mcr-1 positive plasmid and one isolate (14EC033) carrying two mcr-1 positive plasmids. These eight mcr-1 positive plasmids belonged to the IncX4, IncI2, and IncP1 types. In addition, the mcr-1 gene was the solo antibiotic resistance gene identified in the mcr-1 positive plasmids, while the rest of the antibiotic resistance genes were mostly clustered into one or two plasmids. Interestingly, one mcr-1 positive isolate (14EC047) was susceptible to PB, and we showed that the activity of MCR-1-mediated colistin resistance was not phenotypically expressed in 14EC047 host strain. Furthermore, three isolates exhibited resistance to PB but did not carry previously reported mcr-related genes. Multilocus sequence typing (MLST) showed that these mcr-1 positive E. coli isolates belonged to five different STs, and three isolates belonged to ST301 which carried multiple virulence factors related to diarrhea. Additionally, the mcr-1 positive isolates were all susceptible to imipenem (IMP), suggesting that IMP could be used to treat infection caused by mcr-1 positive E. coli isolates. Collectively, this study showed a high occurrence of mcr-1 positive plasmids in patients with diarrheal diseases of Guangzhou in China and the abolishment of the MCR-1 mediated colistin resistance in one E. coli isolate.

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“…Phylogenetic tree was generated using RAxML v.7.8.6. with 1,000 bootstraps (Kallonen et al, ; Shen, Wu, et al, ). MLST analysis was performed to define the MCRPEC ST‐types using the Warwick MLST scheme (Wirth et al, ).…”

Section: Methodsmentioning

confidence: 99%

“…In addition to the extensive use of antibiotics in human disease treatment, antimicrobial consumption in global animal farming, especially extensively subtherapeutic‐dose addition in animal feed, is a very important reason for the rapid dissemination of antibiotic‐resistant Enterobacteriaceae (Johnson et al, ; Liu et al, ). Polymyxins have been regarded as a final line of antimicrobial agents against the serious infections caused by MDR bacteria, such as clinically CRE (Shen, Wu, et al, ). However, this last‐line antibiotic has been challenged by the emerging and global transmission of plasmid‐encoded mcr genes.…”

Section: Introductionmentioning

confidence: 99%

Population structure and antimicrobial resistance traits of avian‐origin mcr‐1 ‐positive Escherichia coli in Eastern China, 2015 to 2017

Zhuge

Tang

et al. 2019

Transbound Emerg Dis

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Recent emergence of mcr-1-positive Escherichia coli (MCRPEC) is causing serious concern around the world. Due to poultry-origin E. coli holding zoonotic potential, the improved understandings of MCRPEC population structure and antimicrobial resistance are critical to public health purposes. This study provided novel insights into the molecular epidemiology of avian-origin MCRPEC. For the mcr genes prevalence study, we analysed 1,360 E. coli recovered from avian colibacillosis in eastern China from 2015 to 2017. The mcr-1 was present in 172 (12.6%) E. coli isolates.For all of MCRPEC isolates, MICs of colistin were ≥4 mg/L. Avian-origin MCRPEC was widely distributed throughout phylogroups A, B1, B2, D, and F. Moreover, those isolates were assigned to 52 unique STs, such as ST48, ST117, ST131, and ST648, suggesting substantial horizontal dissemination of mcr-1 gene through avian-origin E. coli populations. The susceptibility of MCRPEC isolates was tested with 26 antimicrobial agents from 16 antimicrobial categories. There were high resistance rates of MCRPEC isolates against the clinically used antibiotics. All MCRPEC isolates in this study presented the multidrug-resistant (MDR) trait were even considered as extensively drug-resistant (XDR) strains. Resistance genotypes and plasmid replicon profiling showed that a majority of MCRPEC isolates contained plasmid-mediated resistance genes and exhibited the co-existence of mcr-1 with ESBLs and pAmpCs genes. Furthermore, the overlapped distribution of ST types and resistance gene contents was detected among MCRPEC isolates from humans and poultry. Besides mcr-1, our findings highlighted a significant prevalence of plasmid-mediated resistance genes among avian-origin MCRPEC isolates. K E Y W O R D Santimicrobial susceptibility, avian-origin MCRPEC, resistance genotypes, sequence type

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Heterogeneous and Flexible Transmission of mcr-1 in Hospital-Associated Escherichia coli

Cited by 57 publications

References 69 publications

Prevalence and Molecular Characteristics of Avian-Origin mcr-1-Harboring Escherichia coli in Shandong Province, China

Prevalence and Molecular Characteristics of Avian-Origin mcr-1-Harboring Escherichia coli in Shandong Province, China

Antimicrobial Resistance Profile of mcr-1 Positive Clinical Isolates of Escherichia coli in China From 2013 to 2016

Population structure and antimicrobial resistance traits of avian‐origin mcr‐1 ‐positive Escherichia coli in Eastern China, 2015 to 2017

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