Heterogeneous and homogeneous electrochemiluminoimmunoassays of hTSH at disposable oxide-covered aluminum electrodes

Heterogeneous oligonucleotide-hybridization assay based on hot electron-induced electrochemiluminescence of a rhodamine label at oxide-coated aluminum and silicon electrodes

“…The effective area of the electrode in this cell was 63.6 mm 2 . A laboratory-made coulostatic pulse generator [22] was applied to generate cathodic pulses and a pulse generator was adjusted to yield cathodic pulses with 40 C of charge and −40 V of voltage with frequency of 20 Hz. Contact angles were measured with drop shape analysis system (Kruss).…”

“…A distinct advantage of HECL over anodic ECL is that various luminophores with different optical and redox properties can be simultaneously excited [14,20]. HECL has been previously used for biorecognition detection in immunoassays [12,[21][22][23]. Capturing antibodies were physically adsorbed on oxide-coated aluminum [12,21,22] and silicon [23] electrodes and the biorecognition reaction was detected using target antibodies labeled with HECL labels.…”

“…HECL has been previously used for biorecognition detection in immunoassays [12,[21][22][23]. Capturing antibodies were physically adsorbed on oxide-coated aluminum [12,21,22] and silicon [23] electrodes and the biorecognition reaction was detected using target antibodies labeled with HECL labels. Immunoassays and nucleic acid probing techniques share many similarities, as they are both based on a unique biorecognition process, however, there are some fundamental differences due to the fact that antibodies are relatively large molecules (MW ≈ 160 kDa) while DNA-assays utilize short oligonucleotides (MW ≈ 5-10 kDa).…”

Cathodic electrochemiluminescence of lucigenin at disposable oxide-coated aluminum electrodes

“…The measurements were carried out using a coulostatic pulse generator [35] with 120 µC, 20 Hz, -50 V cathodic pulses. The ECL intensities of lucigenin were detected through an interference filter with a center wavelength at 500 nm and transmission band half-width of 10 nm, using single photon counting and apparatus described previously [36,37].…”

Wash-LOCI - A Semi-Heterogeneous Version of the LOCI Technology Allowing Removal of Unbound Material After Each Assay Step

“…A separation could be done by binding antibody-A beads to the well bottom, by a filtration step or by using magnetic beads (Hall et al, 1999;Koskinen et al, 2004;Kulmala et al, 2002;McCrindle et al, 1985;Namba et al, 2000;Obenauer-Kutner et al, 1997;Okano et al, 1992;Sardesai et al, 2009;Sin et al, 2006;Soukka et al, 2001). All the described methods are based on particulate labels.…”