Heterogeneous composition of histochemical fibre types in the different parts of M. longissimus thoracis from Mishima (Japanese native) steers

Morita, S.; Iwamoto, Hisao; Fukumitsu, Y; Gotoh, Takafumi; Nishimura, Shigeyuki; Ono, Yoshitaka

doi:10.1016/s0309-1740(99)00070-4

Cited by 13 publications

(8 citation statements)

References 15 publications

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“…Serial frozen sections (8 µm thick) were cut transversely from the soleus and EDL muscles and stained by the histochemical reactions for myosin adenosine triphosphatase (ATPase) activities after preincubation of the solutions at pH 4.3 or 10.5 and reduced nicotine amide adenine dinucleotide dehydrogenase (NADH-DH) activities ( 22 ). On microscopic photograph ( ϫ 50) taken on the same location of the specimen, myofibers were categorized into type I, IIa and IIb according to the nomenclature of Brooke and Kaiser ( 23 ).…”

Section: Methodsmentioning

confidence: 99%

Beef Extract Supplementation Increases Leg Muscle Mass and Modifies Skeletal Muscle Fiber Types in Rats

Yoshihara

Wakamatsu

Kawabata

et al. 2006

J Nutr Sci Vitaminol

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SummaryThe objective of this research was to investigate the effects of beef extract on fat metabolism, muscle mass and muscle fiber types in rats. We also investigated the synergetic effect of endurance exercise. Twenty-four male rats weighing about 270 g were assigned to two diets containing 0 or 6% beef extract (BE). Half the rats fed each diet were subjected to compulsory exercise (CE) for 30 min every other day. After 4 weeks feeding, the blood was collected and various organs were dissected. The muscle fiber type of the soleus and extensor digitorum longus (EDL) muscles were evaluated by histochemical and electrophoretical analyses. Rats supplemented with BE showed a decrease in fat content in liver and abdomen and an increase in the activity of carnitine palmitoyl transferase II in liver. BE as well as exercise increased the relative weights of both soleus and EDL. BE alone and BE plus CE did not affect the distribution of muscle fiber types in soleus. BE without exercise decreased in type IIb of EDL from 54% to 44% with compensatory increase in type IIa from 41% to 49% and type I from 5% to 7% compared with the nonsupplemented, nonexercised control group. No synergetic effect on a fast to slow fiber conversion due to the combination of BE and CE was detected. Thus, BE supplement increased muscle mass and slow type fiber in EDL. The effects of BE supplement on muscle characteristics were similar to those of exercise. Key Words beef extract, fat metabolism, muscle fiber type, muscle mass, L -carnitine A great deal of research has been done to elucidate the general nutritional value of beef on the growth of human subjects and the effects of protein quality and exercise on the growth and protein metabolism of animals and human beings ( 1-3 ). However, existing information about the nutritional effects of beef extract on muscle metabolism is not sufficient.Skeletal muscle is composed of at least three fiber types on the basis of their contractile and energy metabolic properties; type I fibers exhibit an aerobic energy metabolism (slow-twitch fiber), type IIb fibers are predominantly glycolytic (fast-twitch fiber) and type IIa fibers are the intermediate type exhibiting both oxidative and glycolytic metabolisms. Lipids are easily accumulated in type I fibers ( 4 ), while glycogen depletion occurs primarily in the type II ( 5 ). It is well known that cross-reinnervation, electrical stimulation or exercise evokes the fiber type conversion, although the molecular mechanisms mediating the transformation are not clearly elucidated ( 6 , 7 ). This means that skeletal muscle cells possess a capacity to respond and adapt to metabolic changes imposed by physical stimulation or neural stimulation. Concretely, endurance exercise requiring the consumption of oxygen stimulates a shift to the slow twitch, oxidative muscle fibers ( 8 ). The fiber types of adult skeletal muscle are closely related to energy metabolic characteristics as described above. Nutrition-induced regulation in mRNA levels for a number of metabolic gene...

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Section: Methodsmentioning

confidence: 99%

Beef Extract Supplementation Increases Leg Muscle Mass and Modifies Skeletal Muscle Fiber Types in Rats

Yoshihara

Wakamatsu

Kawabata

et al. 2006

J Nutr Sci Vitaminol

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“…Morita et al [ 7 ] compared the types of muscle fiber in the longissimus muscle between Mishima (a Japanese native) and Japanese Black cattle, including regional variations. The size of type I myofibers in the longissimus muscle were larger in the Mishima cattle, while the population of type I myofibers was less [ 7 , 30 , 31 ]. However, the percentage of type I myofibers in terms of total area was nearly identical between the breeds.…”

Section: Histochemical Properties Of Wagyu Musclementioning

confidence: 99%

The Japanese Wagyu beef industry: current situation and future prospects — A review

Gotoh

Nishimura

Kuchida

et al. 2018

Asian-Australas J Anim Sci

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In Japan, Wagyu cattle include four Japanese breeds; Black, Brown, Shorthorn, and Polled. Today, the renowned brand name Wagyu includes not only cattle produced in Japan, but also cattle produced in countries such as Australia and the United States. In recent years, the intramuscular fat percentage in beef (longissimus muscle) from Japanese Black cattle has increased to be greater than 30%. The Japanese Black breed is genetically predisposed to producing carcass lipids containing higher concentrations of monounsaturated fatty acids than other breeds. However, there are numerous problems with the management of this breed including high production costs, disposal of untreated excrement, the requirement for imported feed, and food security risks resulting from various viral diseases introduced by imported feed. The feeding system needs to shift to one that is more efficient, and improves management for farmers, food security for consumers, and the health environment for residents of Japan. Currently, we are developing a metabolic programming and an information and communications technology (ICT, or Interne of Things) management system for Wagyu beef production as future systems. If successful, we will produce safe, high-quality Wagyu beef using domestic pasture resources while solving the problems of how to utilize increasing areas of abandoned agricultural land and to make use of the plant-based feed resources in Japan’s mountainous areas.

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“…Histochemical staining techniques can be used to describe the complex characteristics of muscle and to identify different muscle fiber types (Morita et al, 2000;Ozawa et al, 2000). Different histochemical identification methods for muscle fiber types have been introduced over the years (Fig.…”

Section: Classification Of Muscle Fiber Typementioning

confidence: 99%

Muscle fiber characteristics, myofibrillar protein isoforms, and meat quality

2009

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Heterogeneous composition of histochemical fibre types in the different parts of M. longissimus thoracis from Mishima (Japanese native) steers

Cited by 13 publications

References 15 publications

Beef Extract Supplementation Increases Leg Muscle Mass and Modifies Skeletal Muscle Fiber Types in Rats

Beef Extract Supplementation Increases Leg Muscle Mass and Modifies Skeletal Muscle Fiber Types in Rats

The Japanese Wagyu beef industry: current situation and future prospects — A review

Muscle fiber characteristics, myofibrillar protein isoforms, and meat quality

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