Heterogeneous Expression of Tumor Necrosis Factor-α Receptors I and II on Human Peripheral Eosinophils

Abstract

Background: Expression of the tumor necrosis factor-α receptor by human eosinophils has not been determined. We examined the surface expression and mRNA for tumor necrosis factor-α receptors I and II (TNF-αRI and TNF-αRII) on peripheral eosinophils. Methods: Eosinophils were obtained from 17 asthma patients and 3 healthy volunteers. Flow cytometry was used to examine receptor expression, and the reverse transcriptase-polymerase chain reaction was used to examine receptor mRNA expression. Results: Flow cytometry revealed that 16 out of 20 subjects had eosinophils expressing TNF-αRI and 18 subjects had eosinophils expressing TNF-αRII. All eosinophils expressed at least one receptor. The mRNA for TNF-αRII was detected in all eosinophils examined, but the signals for TNF-αRI mRNA were only found in cells expressing this receptor. Conclusions: Human peripheral eosinophils show heterogeneous expression of TNF-αRI and TNF-αRII.

Tumour necrosis factor‐α‐induced expression of intercellular adhesion molecule‐1 on human eosinophilic leukaemia EoL‐1 cells is mediated by the activation of nuclear factor‐κB pathway

“…Another study has reported that the expression of ICAM‐1 on another human eosinophilic leukaemia, EoL‐3, could be enhanced by platelet activating factor and platelet factor 4 [31]. Eosinophils have been shown to express TNF receptor I and receptor II [32]. TNF‐α was found to stimulate IkB degradation accompanied with subsequent NF‐kB translocation in many leucocytes [9, 23].…”

Eosinophils: Biological Properties and Role in Health and Disease

“…Activation of these receptors are thought to promote eosinophil apoptosis (Zeck-Kapp et al 1994;Zeck-Kapp & Kapp 1995). While eosinophils have been shown to express a number of IFN receptor superfamily members, including receptors for IFN-a, IFN-b, IFN-g, and IL-10 (Giembycz & Lindsay 1999), only the receptor for IFN-g (type 2 IFN) has been convincingly identified (Aldebert et al 1996;Ishihara et al 1997;Matsuyama et al 1998;Ochiai et al 1999). The IL-2 receptor is composed of three polypeptide chains, an achain (p55) (CD25), b chain (p75) (CD122), and a g chain (CD132) that is common to several other cytokine receptors.…”

Pharmacological Regulation of Human Eosinophil Apoptosis

“…The effects of TNF-α on target cells are generally mediated by two receptors: TNF-RI (p55) and TNF-RII (p75). Eosinophils have been shown to express both of these receptors [114].…”