Heterologous antibodies in human and rat's glioblastoma

Roda, J. E.; Villarejo, F.; Heredero, J.; Obrador, S.

doi:10.1007/bf01405932

Acta neurochir

1976

DOI: 10.1007/bf01405932

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Heterologous antibodies in human and rat's glioblastoma

J. E. Roda¹,

F. Villarejo²,

J. Heredero³

et al.

Abstract: IgG from immunized rabbits was labelled with Fluorescein Isothiocyanate, and conjugated in vitro with human and with rat glioblastoma. After absorption of the antisera with normal brain and liver, a heavy concentration of antibody was found in the rat tumour cells. A great amount of antibody was also found in the human neoplastic cells. The incorporation of this antibody in glioma cells of different rats developing new tumours indicates that transplantation antigens of the H-2 type are not the only ones capabl… Show more

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1978

1981

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Cited by 3 publications

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The utilization of native glioma antigens in the assessment of cellular and humoral immune responses in malignant glioma patients

et al. 1981

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Cellular and humoral components of the immune response have been studied preoperatively, concurrently, and serially in patients with malignant glial neoplasms. In order to assess titres of circulating antibodies to tumour cell constituents an indirect immunofluorescent technique was applied to single cell suspensions and snap frozen cell smears. In an allogeneic system, 49% of 47 test and 7% of 124 control sera gave a positive response to cytoplasmic components. The leucocyte adherence inhibition assay was applied to study 39 test and 64 control patients. Significant non-adherence of leukocytes was observed in 77% of test cases. Control parameters indicated specificity of the response. Simultaneous assessment in 28 test patients yielded a positive response for one or both assays in 89% of cases..

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The utilization of native glioma antigens in the assessment of cellular and humoral immune responses in malignant glioma patients

et al. 1981

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Detection of IgG on glioblastoma cell surfacein vivo

Kirsch

1978

Acta neurochir

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Tumoural antigens on experimental and human glioblastoma

Roda¹,

Heredero²,

Villarejo³

et al. 1980

Acta neurochir

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The fact that glioblastoma multiforme possesses antigens differing from those of normal brain has been stressed in early papers from Scheimberg, Mahaley, Eggers, and Brooks. In our work the presence of specific cytoplasmic and nuclear antigens in neoplastic cells has been demonstrated. These specific antigens are present not only in experimental tumours from the rat, but also in human glioblastoma, and are easily demonstrated by immunodiffusion and immunofluorescence techniques. From our work differences between intracellular and membrane antigens are clear, as the latter do not react with IgG immunoglobulin. On the other hand, tumoural antigens in glioblastoma have similar antigenic qualities to those of histocompatible antigens in normal brain. Experimental and human glioblastomas have weak antigens, as demonstrated by frequent tumour recurrence following amputation and the positive cross-reaction of antibody with normal brain in experimental models. Glioblastoma multiforme may have a common antigen as its antibodies easily cross-react positively with different human tumours with similar, histological features. As tumoural membranes did not react as cytoplasm and nuclei, we cannot say that membrane antigens resemble those of intracellular contents. The fact that viral-induced tumours may have common antigens should point to aetiological possibilities in this group of tumours. Delayed cellular response is very useful during the follow-up of these patients. Positive DNCB and intradermal reactions could be elicited in those patients in whom the antigenic overload has been reduced as a consequence of a surgical procedure. On the other hand, patients with extensive and infiltrating tumoural masses exhibited weak or negative delayed cellular responses. Humoral responses from the patient's sera may not have the prognostic value of cellular responses.

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Heterologous antibodies in human and rat's glioblastoma

Cited by 3 publications

References 5 publications

The utilization of native glioma antigens in the assessment of cellular and humoral immune responses in malignant glioma patients

The utilization of native glioma antigens in the assessment of cellular and humoral immune responses in malignant glioma patients

Detection of IgG on glioblastoma cell surfacein vivo

Tumoural antigens on experimental and human glioblastoma

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